Isolated fracture of the ventricular septum after blunt chest trauma

Isolated rupture of of the ventricular septum after blunt chest trauma is a very rare traumatic affection. A 21-year-old man was admitted to our hospital because of blunt chest trauma and a forearm fracture. Initial echocardiography did not show any intracardiac or extracardiac pathologic lesions, but 12 hours later this examination was repeated because of the onset of a holosystolic murmur. An unusual traumatic rupture of the ventricular septum was demonstrated. The hemodynamically stable condition of the patient allowed surgical repair to be performed 3 months later.     

人类白细胞抗原B27亚型基因与急性前葡萄膜炎的相关性研究

目的 探讨人类白细胞抗原B27亚型基因与急性前葡萄膜炎(acute anterior uveitis,AAU)易感性的关系。 方法 对49例临床确诊的AAU患者,通过聚合酶链反应(polymerase chain reaction, PCR)特异性扩增技术检测B27;以B27阳性者的DNA为模板对其人类白细胞抗原HLA-B的第二、三外显子片段扩增。采用DNA测序技术对扩增产物做基因序列分析,经计算机处理获得受检者HLA-B27亚型的信息。 结果 受检者中29例为B27阳性,占59.39%,其中仅发现B2704(13例,占44.00%)和B27052(16例,占56.00%)二种亚型基因携带者。二者间多数临床表现差异不大,但携带B27052基因的AAU患者伴发强直性脊柱炎(ankylosing spondylitis, AS)的人数(7人,占24.24%)显著高于B2704基因携带者(1人,占3.74%)。 结论 B2704和B27052单独的亚型特异性与AAU无易感性关联;但B27052基因可能与并发AS有关。 (中华眼底病杂志, 1999, 15:139-142)     

反相高效液相色谱法测定塞来昔布片含量

目的 :建立反相高效液相色谱法测定塞来昔布片的含量。方法 :采用ZorbaxSB -C18色谱柱 ,以 0 .0 2mol·L-1磷酸二氢钾缓冲液 (pH5 .8) 乙腈 (4 5 :5 5 )为流动相 ,流速为 1.0mL·min-1,以 5 甲基 2 硝基苯酚为内标物 ,检测波长为 2 5 2nm。结果 :塞来昔布在 10 .2～ 5 0 .1mg·L-1范围内呈良好线性 (r =0 .9998) ,平均回收率为 99.8% ,RSD为 0 .35 %。结论 :本法可用于该片的含量测定 ,操作简便 ,结果准确。     

反义寡核苷酸的化学修饰

反义技术是近20年来发展的一种全新的药物设计方法。本文就近几年来反义寡核苷酸在化学修饰方面，包括骨架修饰、糖环修饰、碱基修饰等的最新发展进行了综述。     

胸段食管癌三维适形放疗摆位误差研究

Objective To study the setup errors in three-dimensional conformal radiotherapy (3DCRT) for thoracic esophageal carcinoma using electronic portal imaging device(EPID) and calculate the margins from CTV to PTV. Methods Forty-one patients with thoracic esophageal carcinoma who received 3DCRT were continuously enrolled into this study. The anterior and lateral electronic portal images (EPI) were aquired by EPID once a week. The setup errors were obtained through comparing the difference between EPI and digitally reconstructed radiographs(DRR). Then the setup margins from CTV to PTV were calculat-ed. By using self paired design,22 patients received definitive radiotherapy with different margins. Group A: the margins were 10 mm in all the three axes;Group B: the margins were aquired in this study. The differ-ence were compared by Paired t-test or Wilcoxon signed-rank test. Results The margins from CTV to PTV in x,y and z axes were 8.72 mm, 10.50 mm and 5.62 mm, respectively. Between the group A and group B, the difference of the maximum dose of the spinal cord was significant(4638.7 cGy±1449.6 cGy vs. 4310.2 cGy±1528.7 cGy; t=5.48, P=0.000), and the difference of NTCP for the spinal cord was also significant (4.82%±5.99% vs. 3.64%±4.70%;Z=-2.70,P=0.007). Conclusions For patients with tho-racic esophageal carcinoma who receive 3DCRT in author's department,the margins from CTV to PTV in x, y and z axes were 8.72 mm, 10.50 mm and 5.62 mm, respectively. The spinal cord could be better protected by using these setup margins than using 10 mm in each axis.     

核素骶髂关节显像对强直性脊柱炎治疗监测的价值

目的 利用核素骶髂关节显像以及骶髂关节与骶骨的核素比值测定方法，探讨其在强直性脊柱炎(AS)治疗监测中的价值。方法 29例临床及CT确诊AS的病人，共58个骶髂关节，先行核素骶髂关节显像及其核素比值测定，然后进行临床药物治疗。治疗后2周再行同样的核素检查方法，观察其变化情况。结果 58个骶髂关节中，有51个骶髂关节示放射性核素呈不同程度浓聚，骶髂关节与骶骨放射性比值明显高于正常；2周后的检查所见其中原有异常的骶髂关节情况明显改善，有51个骶髂关节与骶骨比值均有降低，其中47个降至正常范围。两组比较差异有显著意义。结论 核素骶髂关节显像及其骶髂关节与骶骨核素比值测定，对骶髂关节炎的活动性监测具有重要的应用价值。     

高效液相色谱法测定舒金克喘胶囊中和厚朴酚与厚朴酚的含量

目的：测定舒金克喘胶囊中和厚朴酚与厚朴酚的含量。方法：色谱条件：色谱条件与系统适用性实验：Hypersil ODS2 C18分析柱（150mm 4．6mm，5μm）；流动相：甲醇：水=（78：22），检测波长294nm流速1．0ml／min，室温操作。理论塔板数按厚朴酚计算应不低于3000。结果：和厚朴酚与厚朴酚平均回收率分别为99．62%（RSD=1．34％），98．56％（RSD=1．49%）。结论：方法快速准确，样品处理简便易行。     

非小细胞肺癌中DPC4基因的表达与微血管形成的关系

目的：探讨DPC4（deleted in pancreatic carcinoma locus4，DPC4）基因在非小细胞肺癌（NSCLC）中的表达及其与微血管形成的关系。方法：利用免疫组织化学S-P法检测52例NSCLC组织、19例相应的癌旁正常肺组织中DPC4、VEGF的表达，用CD34标记血管内皮细胞并计算微血管密度MVD值。结果：DPC4在肺癌原发灶中的阳性表达率为63．5％（33／52），与癌旁正常肺组织中的阳性表达率89．5％（17／19）相比，DPC4阳性表达水平显著降低（P〈0．05）；DPC4与组织学类型、肿瘤细胞分化程度无关（P〉0．05），但与淋巴结转移显著相关（P〈0．05）。52例NSCLC中，DPC4的表达与VEGF、MVD值均呈负相关（r=-0．303，P=0．020）。结论：DPC4的低表达可能是肺癌发生过程的早期事件，可促进肺癌的淋巴结转移，并可通过直接或间接的作用促进肺癌血管生成。