大鼠骨髓间充质干细胞分化为神经干细胞

为了观察骨髓间充质干细胞(BMSCs)分化为神经干细胞(NSCs)的能力,本研究通过贴壁法培养大鼠BMSCs,体外培养扩增纯化后,在细胞传代时用含有表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)、N2、B27的DMEM/F12的培养液制成细胞悬液,并进行诱导,观察诱导后细胞的形态及生长情况,用免疫荧光检测形成的细胞球的巢蛋白(nestin)的表达情况;形成的细胞球在含10%血清的培养液中进一步分化。结果显示:BMSCs在含EGF、bFGF、N2、B27的培养液中,逐渐形成nestin表达阳性的细胞球,在含血清的培养液中能分化为神经元样细胞、星形胶质样细胞及少突胶质样细胞。本研究结果提示经纯化的BMSCs能分化为NSCs,并具有进一步分化的能力。     

力尔凡治疗恶性胸腔积液的临床分析

目的　评价力尔凡治疗恶性胸腔积液的疗效及毒性。方法　 41例恶性胸腔积液患者应用力尔凡的腔内给药治疗。结果　治疗有效率 80 % ,毒性反应低。结论　力尔凡治疗恶性胸腔积液的疗效确切 ,能较好改善生存质量 ,且毒性低 ,值得临床应用。     

急性细菌性痢疾335例临床及病原学分析

目的:了解北京市海淀区急性细菌性痢疾临床特点、菌群特点及耐药情况。方法:分析临床诊断为急性细菌性痢疾335例患者的临床特点;对粪便标本进行细菌学培养,培养出志贺氏菌属阳性标本做药物敏感试验。结果:335份粪便标本检测出细菌66株,其中志贺氏菌属30株,致泻性大肠埃希氏菌属8株,变形杆菌属17株,副溶血性弧菌属10株,金黄色葡萄球菌1株,未检出沙门氏菌。检出的志贺氏菌株对头孢噻肟、左氧氟沙星、头孢唑啉敏感,对氨苄青霉素、萘啶酸、四环素、利福平耐药。结论:北京市海淀区急性细菌性痢疾致病菌以志贺氏菌属为主,对头孢噻肟、左氧氟沙星、头孢唑啉敏感,可作为细菌性痢疾首选药物。     

碱性成纤维细胞生长因子、转化生长因子-β1及其受体表达与结直肠癌临床病理特征的关系

背景：血管生成是肿瘤生长、侵袭和转移的关键步骤，其过程受多种血管生成因子调控。目的：研究血管生成因子碱性成纤维细胞生长因子（bFGF）、转化生长因子（TGF）-β1及其受体TβR Ⅰ 在结直肠癌中的表达和意义。方法：应用免疫组化方法检测60例结直肠癌组织中bFGF、TGF-β1和TβR Ⅰ的表达，并与20例正常结直肠黏膜进行比较，分析三者的表达与结直肠癌临床病理特征的关系。结果：结直肠癌组织中bFGF、TGF-β1和TβR Ⅰ表达阳性率增高，与正常结直肠黏膜比较有显著差异（P〈0．05）；且三者的表达与肿瘤Dukes分期、浸润深度和淋巴结转移有关（P〈0．05）。结论：bFGF、TGF-β1和TβR Ⅰ在结直肠癌的发生、发展和转移中起重要作用，可作为判断结直肠癌生物学行为的重要指标。     

新生儿肺透明膜病的超声诊断

目的评价新生儿肺透明膜病（ＨＭＤ）的声像图特征。方法对临床怀疑为ＨＭＤ２２例早产儿和４０例正常足月新生儿的肺部进行超声检查,并分别与临床资料和Ｘ线胸片做比较。结果２２例早产儿中,１７例超声检查显示在肝脾后有弥漫的高强回声。所有病例经Ｘ线胸片或尸检证实为严重的ＨＭＤ;３例在肝脾后呈放射状强回声,Ｘ线胸片提示为轻型的ＨＭＤ;２例缺少这种典型的强回声,Ｘ线诊断为正常肺。而４０例正常新生儿,有３４例在肝脾后未见任何回声,Ｘ线胸片提示心肺正常;６例显示在肝脾后可见稀疏的中等回声,Ｘ线胸片提示湿肺。结论超声检查可做为诊断ＨＭＤ的一种新方法,它具有实用、简便、无创伤并可重复检查的优点     

表皮生长因子酪氨酸抑制剂联合奥沙利铂对肝癌细胞Hep-G2作用

目的探讨表皮生长因子受体(epidermal growth factor receptor,EGFR)抑制剂易瑞沙联合奥沙利铂对人肝癌细胞株增殖和细胞凋亡的影响,为原发性肝癌分子靶向治疗和联合化疗提供临床依据。方法利用噻唑蓝(MTT)法测定奥沙利铂、易瑞沙对细胞增殖的抑制作用及两药联合时的细胞杀伤作用。重复试验3次,每次检测5孔细胞,将平均值作为最终结果,并用中效法则判定两药合用的效果。采用流式细胞仪测定易瑞沙单药应用于Hep-G2细胞,对细胞凋亡的影响。结果易瑞沙联合奥沙利铂增加化疗药对Hep-G2细胞的抑制作用,并呈时间剂量依赖性。易瑞沙单药作用于Hep-G2细胞,发生了细胞凋亡。结论易瑞沙与奥沙利铂的联合应用有望成为治疗肝癌的有效手段。     

Well-Organized MZT-ME-PML Approach for THz BP Metasurface Implementations

Via adopting the matrix exponential (ME) approach, efficient and tightcomplex-frequency-shifted perfectly matched layer (CFS-PML) formulations using thematched Z-transform (MZT) method are well organized and proposed to truncate themonolayer black phosphorous (BP) metasurface problems in the infrared regime. Dueto introducing ME approach, the proposed MZT-ME-PML develops into the compactfirst-order differential matrix form used to the circumvent convolution manipulations,the variable replacements, or the formulation rearrangements during the mathematicalderivations. Furthermore, the MZT-ME-PML can not only flexibly truncate arbitrarymaterials due to using the DB constitutive relation, but efficiently attenuate evanescent waves and reduce late-time reflections with the CFS scheme. Besides, in view ofthe discretization effect on Terahertz (THz) BP metasurface implementation, the MZTtechnique is added deliberately into this proposal in that it is more suitable for simulating fine structures. Finally, the ME-based proposal can maintain high absorptionwhen it comes closer to terminate finite-difference time-domain (FDTD) domains sothat smaller physical regions can be applied, leading to palpable improvements inmemory and CPU time. The three-dimensional (3D) numerical simulations on monolayer BP THz problems have been carried out to illustrate the validity and accuracy ofthe proposal.     

Endoplasmic reticulum stress is involved in okadaic acid-induced tau phosphorylation and neurotoxic effects of okadaic acid

The role of the endoplasmic reticulum in Alzheimer’s disease pathogenesis remains poorly understood. The present study investigated the correlation of okadaic acid-induced tau hyperphosphorylation and neurotoxicity with endoplasmic reticulum stress. Following co-culture of various concentrations of okadaic acid (25, 50, 100, and 200 nmol/L) and human neuroblastoma SH-SY5Y cells, okadaic acid, a selective protein phosphatase (PP-1, PP-2A) inhibitor, reduced cell viability in a concentration-dependent manner,...