Amelioration of streptozotocin‐induced diabetes mellitus,oxidative stress and dyslipidemia in rats by tomato extract lycopene

The effects of various doses of lycopene were studied in streptozotocin (STZ)‐induced hyperglycaemic rats to evaluate its possible hypoglycaemic, hypolipidaemic and antioxidant activity in diabetes. Compared to the normoglycaemic group, the treatment of rats with a single dose of STZ (65?mg/kg body weight) revealed a significant increase (p<0.05) only in plasma hydrogen peroxide (H₂O₂), i.e. by 230?%; it increased the thiobarbituric acid reactive substances (TBARS) as index of the lipid peroxidation level by 69?%, while total antioxidant activity was decreased by 36?%, with a consistently significant decrease (p<0.05) in the activity of erythrocytes antioxidative enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). The levels of total lipid, triglycerides and total cholesterol in serum of hyperglycaemic rats were increased by 14?%, 65?% and 36?%, respectively, while HDL‐C decreased by 22?% compared to the normoglycaemic group. Exogenous administration of individual gradual doses of lycopene to hyperglycaemic rats causes a dose‐dependent decrease in glucose level, an increase of insulin concentration, a decrease of H₂O₂ and TBARS levels, as well as increased total antioxidant status with increased antioxidant enzyme activities (CAT, SOD and GPx) with improvement in serum lipid profile. It is obvious from this study that lycopene acts as an antidiabetic agent through lowering the free radical and has an improving effect on serum that reaches the normal level; the greatest effect of lycopene was observed at 90?mg/kg body weight.     

Patterns of use of the female condom after one year of mass marketing.

The female condom is an effective new contraceptive method that can reduce HIV transmission. This study examines use of the female condom after 1 year of its mass marketing and compares this with use of the male condom. It is based on exit interviews conducted among a random sample of male and female customers visiting outlets that sell the female condom. Compared with reported use of the male condom, which was five to eight times as high in nonmarital as in marital partnerships, reported use of the female condom varied less by partnership type (it was twice as high in nonmarital compared with marital partnerships). In marital and regular partnerships, use of the female and the male condom increased with socioeconomic status (SES). In casual partnerships, use of the male condom increased with SES, but use of the female condom was higher for those with lower SES. Men reported higher levels of male condom use than women, but there were no gender differences in use of the female condom. These findings suggest that the largest contribution to HIV protection through use of the female condom may be within marital partnerships and among low SES men and women who engage in casual sex. The absence of gender differentials in use of the female condom suggests that women are able to exert greater control over the use of the female condom than they are over the use of the male condom. However, the overall low levels of female condom use among relatively affluent persons at outlets that sell the female condom indicate that the introduction of the female condom will be resource intensive.     

Comparative study between direct analysis in whole blood,oral fluid,and declaration of consumption for the prevalence of nonsteroidal anti-inflammatory drugs and acetaminophen in ultratrail runners

Ultratrail running is a sport with growing number of adherents. To complete ultratrail despite physical issues such as joint and muscle pain, many runners use nonsteroidal anti-inflammatory drugs (NSAIDs) and acetaminophen. Studies asking participants about their consumption of drugs during ultratrail revealed a prevalence of NSAIDs and acetaminophen up to 70% and 25%, respectively. The aims of the present study were to determine the prevalence of NSAIDs and acetaminophen for 81 runners during the 2021 Ultratrail du Mont Blanc® (UTMB®) using direct analysis of dried blood spots (DBS) and oral fluid (OF) and to compare results with the declaration of consumption by runners; this is to identify the most relevant method to study the prevalence of drugs. Our results show a prevalence of NSAIDs of 46.6% using DBS, 18.5% using OF, and 13.8% based on a questionnaire. Prevalence of acetaminophen were 30.1%, 30.9%, and 22.5% using DBS, OF, and questionnaire, respectively. From this study, we conclude that the analysis of drugs directly in DBS is the most relevant tool to determine the prevalence in ultratrail events.     

The other colon cancer: a population-based cohort study of appendix tumour trends and prognosis

Aim

Appendiceal neoplasms are rare subtypes of colorectal tumours that mainly affect younger patients some 20 years earlier than other colon tumours. The aim of this study was to gain more insight into the histological subtypes of this rare disease and include cases previously excluded, such as mucinous neoplasia.

Method

The cohort study included 1097 patients from the Munich Cancer Registry (MCR) diagnosed between 1998 and 2020. Joinpoint analysis was used to determine trend in incidence. Baseline demographic comparisons and survival analyses using competing risk and univariate/multivariate methods were conducted according to tumour histology: adenocarcinoma (ADENO), neuroendocrine neoplasia (NEN), mixed adeno-neuroendocrine carcinoma (MANEC), and low- (LAMN) and high-grade mucinous neoplasia (HAMN).

Results

Up to 2016 the number of cases increased significantly [annual per cent change (APC) = 6.86, p < 0.001] followed by a decline in the following years (APC = −14.82, p = 0.014; average APC = 2.5, p = 0.046). Comparison of all patients showed that NEN (48.4%) and mucinous neoplasms (11.6%) had a considerably better prognosis than ADENO (36.0%) and MANEC (3.0%, p < 0.0001). A multivariate analysis within the NEN and ADENO subgroups revealed that further histological classification was not prognostically relevant, while older age and regional tumour spread at diagnosis were associated with a poor prognosis. ADENO histology with high tumour grade and appendectomy only was also associated with poorer survival.

Conclusion

Appendiceal neoplasms are histologically heterogeneous; however, this diversity becomes less relevant compared with the marked difference from cancers of the remaining colon. The previously observed increase in cases appears to be abating; fewer cases of appendicitis and/or appendectomies or changes in histopathological assessment may be behind this trend.     

Gain in growth after surgical repair of congenital heart disease among children with Down syndrome

Down syndrome (DS) is a common genetic disorder among Saudi children, for which limited data is available on growth for those who undergo surgery for congenital heart disease (CHD). We assessed the magnitude of growth over time following surgery and the factors associated with growth change. Eighty-eight children with DS and CHD who underwent surgery between 2009 and 2017 at the tertiary children's hospital in Al-Qassim, Saudi Arabia, were eligible. Information on gender, height, and weight (at surgery, 6 and 12 months), age at surgery, and type of CHD were abstracted from the medical records. Repeated measure analysis of variance (ANOVA) was used for data analysis. The sample's mean age (SD) was 16.2 months (20.1); 47% were male. Height, weight, and body mass index (BMI) increased significantly at both follow-up points. There was a significant interaction between age at surgery, type of heart disease, and time for all three physical indices (p-values <0.001). The increase in height, weight, and BMI was significantly greater for children ≤8 months old with acyanotic CHD compared to children >8 months with cyanotic CHD. Overall, the gain in growth was greater for the children with DS who had had surgery at a younger age.