简套式荧光RT-PCR检测患者血清样本中SARS冠状病毒

目的　建立一种快速、特异、灵敏的SARS冠状病毒(SARS CoV)核酸检测方法。方法　根据GenBank中SARS CoV基因序列,自行设计引物、荧光探针,在PE 770 0扩增仪上探讨工作参数,形成试剂盒,并用研制的试剂检测76份临床SARS样本。结果　简套式荧光RT PCR方法对血清样本、漱口液样本、正常人样本检出率分别为33.3% (12 36 )、6 7.5 % (2 7 4 0 )、0 (0 / 16 0 ) ,与经典套式检测结果一致。而传统一步法荧光RT PCR对样本的检出率分别为13 9% (5 36 )、5 2 5 % (2 1 4 0 )、0 (0 / 80 )。结论　简套式荧光RT PCR核酸检测法是SARS临床早期诊断快速、特异、灵敏的方法。     

Expression and function of c-kit receptor in bone marrow mononuclear cells of patients with myelodysplastic syndromes

目的　了解骨髓增生异常综合征 (MDS)骨髓单个核细胞c kit受体的表达与功能。方法　采用免疫荧光方法测定c kit受体蛋白 (CD117)的表达 ;逆转录 聚合酶链反应方法测定c kitmRNA的表达 ;细胞培养检测c kit受体的功能。结果　CD117表达率正常人为 3 0 4 %± 1 4 9% ,MDS患者为 8 58%± 5 2 8% ,两者差异有显著性 (P <0 0 5) ;RA患者为 5 12 %± 2 13% ) ,RAEB RAEB t患者为 10 0 1%± 5 0 7% ,两者差异有显著性 (P <0 0 5) ;MDS继发白血病患者为 32 4 3%± 18 16 %。MDS患者c kit基因mRNA表达与CD117表达相一致。正常骨髓单个核细胞体外半固体培养 ,在加入造血干细胞因子、白细胞介素 3、红细胞生成素 (SCF IL 3 Epo)后形成的粒 巨噬细胞集落 (CFU GM)较仅加入IL 3 Epo显著增多 (P <0 0 5) ,红系爆式集落 (BFU E)数量极显著增多 ,且BFU E体积显著增大(P <0 0 1)。MDS患者在上述相同条件下CFU GM数量无明显变化 (P >0 0 5) ,与正常对照比较 ,CFU GM和BFU E数量均显著减少 (P <0 0 5)。结论　MDS患者骨髓单个核细胞CD117表达明显高于正常对照 ,且与病情进展相关 ;c kitmRNA表达与CD117表达相一致 ;MDS患者骨髓单个核细胞体外培养时 ,SCF协同IL 3、Epo促进造血干 祖细胞增殖分化能力较正常对照明     

CT-guided percutaneous thymus microwave radiation and local injection of dexamethasone for myasthenia gravis

Myastheniagravis(MG)iscommoninyoungin-dividuals,anditseriouslyaffectstheirdailyworkandlife.Althoughthymectomyhasmarkedtherapeuticef-fect,itisnotindicatedforallMGpatients.Besides,itistraumaticandliabletocausecomplications.ThymusradiotherapyisanalternativeforMGmanagement,butitstherapeuticeffectisnotstableandpersistent.Also,radiationmaycauseinjury.WereportanewtherapyforMGbyusingCT-guidedpercutaneousparacentesistothethymusviatheuppermarginofthesternumin-cisurewith2450MHzmicrowaveradiationand…     

重组人钙调素对白芷细胞增殖及结核杆菌生长的影响

目的 :探讨重组人钙调素 ( rh Ca M)对白芷悬浮细胞及结核杆菌增殖作用的影响。　方法 :用基因重组技术获得人钙调素基因 ( h Ca M c DNA)重组表达质粒 h Ca M / p BV2 2 0 ,将其转化大肠杆菌 DH5α。用 Phenyl-Sepharose CL -4 B疏水亲和层析法纯化重组菌超声上清表达产物。将纯化 rh Ca M加入悬浮培养的白芷细胞及结核杆菌培养基中 ,观察 rh Ca M对其增殖作用的影响。　结果 :含 h Ca M / p BV2 2 0的重组菌经温度诱导可高效表达Ca M蛋白 ,经 15 % SDS-PAGE分析 ,可观察到一相对分子质量为 170 0 0的表达条带 ,Western blot结果证实 ,此表达条带可与标准鼠抗 Ca M Mc Ab起特异反应。每 1L菌液经 Phenyl-Sepharose CL -4 B疏水亲和层析法纯化可获Ca M纯品 3～ 4mg。rh Ca M对白芷细胞增殖作用影响的研究结果表明 ,rh Ca M在低细胞密度培养条件下对白芷细胞有促进增殖作用 ,效果高于标准植物 Ca M。本研究同时还发现一定浓度的纯化 rh Ca M( 1、10μg/ ml)可促进牛型结核杆菌的生长。　结论 :rh Ca M对植物细胞和细菌均有细胞外促增殖作用     

经皮球囊二尖瓣成形术疗效分析

本文分析经皮球囊二尖瓣成形术治疗风湿性二尖瓣狭窄的疗效。结果表明 ,PBMV耗费少 ,住院期短。效果良好 ,值得推广应用     

基于关键规则分组优先提取策略的完备化算法及其实现

在分析现有的完备化策略的基础上提出了关键规则分组优先提取策略,介绍了基于此策略的完备化算法及其具体实现,初步探讨了策略的选择对于提高此类系统演算效率的重要作用。     

肾功能分级在成人心脏术后肾功能衰竭的应用

Objective To evaluate the ability of the RIFLE classification to predict hospital mortality in adult patients who underwent cardiac surgery. Methods From October Ist 2006 to December 31st 2006, five hundred and nine adult patients who underwent coronary artery bypass grafting and/or valve operation were enrolled in this study. Renal function was assessed daily according to the RIFLE classification, meanwhile, APACHE Ⅱ score and SOFA score were also evaluated, as well as the maximum scores were recorded. Results Mean duration of ventilation support was 18(14 - 19) hours, the time of ICU stay was 1.4 ± 1.0 days, and the time of postoperative hospital stay was 12. 0(10.0- 15.0) days. 167 patients (32. 8%) incurred postoperative ARF according to the RIFLE classification. The overall mortality was 4. 3% (22/502). A significant increase (P < 0. 01) was observed for mortality based on RIFLE classification. By applying the area under the receiver operating characteristic curve, the RIFLE classification had more powerful discrimination power [0. 933, (95% CI 0. 872 -0. 995) ,P <0. 001]. Conclusions ARF is one of the major complications in postcardiotomy patients. Analytical data suggested the good discriminative power of the RIFLE classification for predicting inpatient mortality of adult postoperative patient with ARF, and the RIFLE classification is simple and practically performed. According to the RIFLE classification, patients with RIFLE class I or class F incur a significantly increased risk of in-hospital mortality compared with those who never develop ARF.     

大鼠内关注射肾上腺素对血流动力学与血药浓度的影响

目的：研究大鼠内关穴注射肾上腺素对血流动力学参数和血浆肾上腺素含量的影响。方法：采用直接插管、微机测取血流动力学指标法以及高效液相色谱法测定血浆肾上纱含量。结果：内关穴注射肾上腺素后,血压迅速上升,反映心肌收缩性能的ｄｐ／ｄｔｍａｘ,Ｖｐｍ和Ｖｍａｘ等指标增大,药理效应与静脉给药相似,而血浆肾上腺素浓度明显低于静脉给药组（Ｐ〈０．０１）。结论：内关穴可能存在接受与传递肾上腺素信息的中介物质和途径。     

水溶性O-羧甲基壳聚糖的制备

目的 :合成一种完全水溶的壳聚糖衍生物— O-羧甲基壳聚糖。方法 :在碱性条件下 ,用氯乙酸修饰粉末状的壳聚糖 ,得到产物。结果 :产物经元素分析、FT-IR分析 ,证实其结构与预期相吻合。结论 :该法所得 O-羧甲基壳聚糖具有优越的水溶性能     

敏感期内、末单眼斜视和剥夺猫视觉系统脑源性神经营养因子表达机理研究

目的观察和研究敏感期内、末不同时限单眼斜视
(monocularstrabismus,MS)和单眼剥夺(monoculardeprivation,
MD)幼猫视皮质、外侧膝状体的脑源性神经营养因子(brain
derivedneurotrophicfactor,BDNF)的可塑性变化。从形态学、分
子神经生物学角度探讨不同时限MS和MD幼猫视