Safety and immunogenicity of adjuvanted recombinant subunit herpes zoster vaccine in lung transplant recipients

Lung transplant recipients are at high risk for herpes zoster and preventive measures are a significant unmet need. We investigated the safety and immunogenicity of two doses of a recombinant zoster vaccine (RZV) in lung transplant recipients (≥50 years). We enrolled 50 patients of which 49 received at least one vaccine dose. Anti-glycoprotein E (gE) antibody levels (n = 43) increased significantly compared to baseline (median optical density [OD] 1.96; interquartile range [IQR]: 1.17–2.89) after the first (median OD 3.41, IQR 2.54–3.81, p < .0001) and second vaccine dose (median OD 3.63, IQR 3.39–3.86, p < .0001). gE-specific polyfunctional CD4+ T cell frequencies (n = 38) also increased from baseline (median 85 per 10⁶ CD4+ T cells; IQR: 46–180) to the first (median 128 per 10⁶ CD4+ T cells; IQR: 82–353; p = .023) and after the second dose (median 361 per 10⁶ CD4+ T cells; IQR: 146–848; p < .0001). Tenderness (83.0%; 95%CI: 69.2–92.4%) and redness (31.9%; 95%CI: 19.1–47.1%) at injection site were common. One rejection episode within 3 weeks of vaccination was observed. This is the first study demonstrating that RZV was safe and elicited significant humoral and cell-mediated immunity in lung transplant recipients. RZV is a new option for the prevention of shingles in this population.     

卵巢硬化性间质瘤1例

患者女,27岁,已婚,孕0产0,因“体检发现右侧卵巢肿物18天”入院;既往月经紊乱4年余。妇科查体:阴道少量白色分泌物,宫颈中度糜烂。实验室检查:雌二醇75.69 pg/ml,孕酮0.95 ng/ml。MRI:右侧附件区见2.1 cm×3.2 cm×3.5 cm团块状异常信号,界清,T1WI呈等信号,脂肪抑制T2WI呈等、稍高、高混杂信号(图1A),周边以等信号为主,呈乳头状、梳状深入中央高信号区,高信号区内见团块状、结节状等信号灶,形成“湖岛”征;DWI(b=1000 s/mm 2)呈稍高信号;增强扫描动脉期病灶明显不均匀强化(图1B),静脉期呈渐进性明显强化(图1C),盆腔少量积液。     

社会活动对老年人认知功能的影响

目的 探讨社会活动对不同性别和年龄的中国老年人认知功能的影响。方法 运用2018年“中国老年健康影响因素跟踪调查”的15424份数据。通过MMSE-R量表评价老人认知功能。控制居住地、受教育水平、配偶、自评健康状态、慢性病等因素,运用二元logistic回归分析社会活动对不同性别、年龄段老人认知功能的作用。结果 我国60岁以上老年人认知障碍发生率为34.88%,串门/与朋友交往、家务、看电视听广播和棋牌是所有性别和年龄段的老人认知功能的保护因素。阅读书报是＞85岁组男性老年人认知功能的保护因素,健身运动、旅游是60~85岁组男性认知功能的保护因素;社会组织活动是＞85岁组女性老人认知功能的保护因素。结论 根据老人的状态和需求提供适宜的社会活动方案,重视老年人社会活动的指导和推广。     

GAG-augmented polysaccharide hydrogel: a novel biocompatible and biodegradable material to support chondrogenesis

The quality of articular cartilage engineered using a cell-polymer construct depends, in part, on the chemical composition of the biomaterial and whether that biomaterial can support the chondrocytic phenotype. Acknowledging the supportive influence of tissue-specific matrix molecules on the chondrocytic phenotype, we have combined chondroitin sulfate-A (CSA) and chitosan, a glycosaminoglycan (GAG) analog, to develop a novel biomaterial to support chondrogenesis. Chitosan is a polycationic repeating monosaccharide of beta-1,4-linked glucosamine monomers with randomly located N-acetyl glucosamine units. Chitosan may be combined with the polyanionic CSA such that ionic crosslinking results in hydrogel formation. Bovine primary articular chondrocytes, when seeded onto a thin layer of CSA-chitosan, form discrete, focal adhesions to the material and maintain many characteristics of the differentiated chondrocytic phenotype, including round morphology, limited mitosis, collagen type II, and proteoglycan production. Our findings suggest CSA-chitosan may be well suited as a carrier material for the transplant of autologous chondrocytes or as a scaffold for the tissue engineering of cartilage-like tissue.     

睫状前动脉的巨微解剖及其临床意义

对30只成人和6只新生儿眼球的睫状前动脉的数目、起点外径、长度、行径及侧支吻合进行观测.上、下、内睫状前动脉多为二支,各占76.7%;73.3%;83.3%.外睫状前动脉多为一支(76.6%).上、下、内、外睫状前动脉的长度分别为5.58土0.44mm;4.79士0.72mm;4.43土0. 76mm;8.00土1.07mm.起点外径分别为0.20mm;士0.04mm;0.I8士0.02mm;0.21土0.03mm.在巩膜表面和虹膜大环处睫状前动脉的侧支之间以及侧支与睫状后长动脉之间相互吻合,这些吻合在眼前节侧支循环方面起一定作用.文中对眼直肌手术时如何减少或避免睫状前动脉损伤等问题做了探讨.     

肱桡肌皮瓣的应用解剖学研究

通过尸体解剖,观察了64侧肱桡肌的形态及其主要供血血管——桡侧返动脉的来源、起始部位、主干长度、外径、行经及分支分布。通过动脉内墨汁注射,观察了桡侧返动脉在肱桡肌皮瓣的皮肤供血范围。认为可以桡侧返动脉为蒂的肱桡肌皮瓣转移复盖肘关节的暴露伤及邻近的软组织缺损。也可制成带蒂的肌皮瓣作游离移植。文内讨论了该瓣的优缺点及转移复盖的范围。     

不同功率半导体激光对声带热损伤的比较

目的：探讨Diomed－25半导体激光不同功率对声带热损伤的程度,为临床应用激光治疗声带病变提供实验依据。方法：选用犬作为实验动物,选定激光功率分别为5、10、20W,照射时间为2s,以接触式照射活体犬的声带后,测量组织热损伤的浓度和宽度,并通过大体标本、光镜下观察声带局部损伤情况。结果：照射时间为2s,激光输出功率为5、10、20W时,其组织热损伤的深度分别为0．2－0．4、0．4－0．6、0．8－1．0mm;热损伤的宽度分别为1．0－1．7、1．7－2．0、2．0－2．6mm。在作用时间相同情况下,激光功率不同,对声带组织的热损伤程度有显（P＜0．05）。结论：选择合适功率的半导体激光对声带病变的治疗是安全、有效的。     

一种新的鼠大脑局部缺血模型

目的 制成鼠大脑局部缺血模型。方法 采用左侧颈动脉离心端插管,灌注一定压力和流量的生理盐水。结果 ①美蓝染色显示大脑左侧大部分区域被染蓝,除皮层着色较浅外,其余部位着色较深。②体感诱发电位在灌注后30min时,其幅度超过灌注前20％。结论 染色说明大脑左侧为缺血区,体感诱发电位幅度的增高,表明大脑处于兴奋性损伤状态。证实了此方法制成的大脑局部缺血可靠,且省时省力简便易行。