中国东北地区未经抗病毒治疗的HIV/AIDS患者HIV毒株的耐药基因变异研究

目的　研究我国东北地区未接受抗逆转录病毒治疗的HIV AIDS患者HIV毒株的逆转录酶和蛋白酶耐药变异情况 ,为开展大规模临床抗病毒药物治疗提供本底数据。方法　RT PCR和套式PCR扩增HIVpol区基因 ,双脱氧法测定逆转录酶和蛋白酶基因序列 ,与国际耐药数据库比对辨别耐药变异。结果　 (1) 5 3例患者毒株亚型分析结果 :B′亚型 4 7例 ,B′ C亚型 4例 ,A、B亚型各 1例 ;(2 )未发现逆转录酶和蛋白酶原发耐药变异存在 ,但发现存在逆转录酶抑制剂继发变异 :M4 1L(1.9% )、I6 3M (1.9% )、L74I (1.9% )、S6 8G (1.9% )、V75L (3.8% )、V10 6I (1.9% )、I135L T (5 .7% )、V179D (7.5 % )和V189I (1.9% ) ,无症状感染者RT继发耐药变异出现率为 11.8% ,而艾滋病患者为5 2 .6 % (P <0 .0 1)。存在大量蛋白酶耐药继发变异V77I (88.7% )、L6 3P (86 .8% )、E35D (81% )、A71V(2 4 .5 % )、R4 1K (15 .1% )、L10I (9.4 % )、R5 7K (9.4 % )、D6 0E (9.4 % )、N37D (5 .7% )、G16E (3.8% )、I15V (1.9% )、M36I (1.9% )、K5 5R (1.9% )和L89M (1.9% )。未发现明显的亚型特异性耐药变异。结论　在中国东北地区未接受抗逆转录病毒治疗的HIV AIDS患者中未发现毒株逆转录酶和蛋白酶耐药原发变异 ,但大量继发耐药变异的存在提     

Randomized, open-label study of the impact of two doses of subcutaneous recombinant interleukin-2 on viral burden in patients with HIV-1 infection and CD4+ cell counts of > or = 300/mm3: CPCRA 059

The effect of intermittent courses of recombinant interleukin-2 (rIL-2) on HIV-1 load in patients receiving combination antiretroviral therapy remains uncertain. CPCRA 059 was an open-label, randomized, multicenter trial in which 511 patients with HIV-1 infection and CD4+ cell counts of > or = 300/mm3 who were receiving antiretroviral therapy were assigned to receive no rIL-2 (255 patients [controls]) or subcutaneous rIL-2 in dosages of 4.5 MIU (130) or 7.5 MIU (126) twice daily for 5-day courses every 8 weeks to maintain CD4+ cell counts that were twice the baseline value or > or = 1,000/mm3. The primary objective of this study was to compare the effects of the two doses of rIL-2 and no rIL-2 on viral load and CD4+ cell counts over 12 months. There was no difference in the following viral load measurements between the rIL-2 treatment groups and the control treatment group: percentage of patients with viral loads of <50 copies/mL at 12 months (p =.55), time to viral load of > or = 50 copies/mL for patients who had baseline viral loads of <50 copies/mL (p =.35), and change in viral load from baseline for patients who had viral loads of > or = 50 copies/mL at baseline (p =.63). At each follow-up visit, the change in CD4+ cell count from baseline was significantly greater in the rIL-2 treatment groups than in the control treatment group, with a mean difference of 251/mm3 at month 12 (95% confidence interval, 207-295; p <.0001). No unanticipated adverse experiences were seen in this trial, to our knowledge the largest randomized evaluation of rIL-2 treatment conducted to date.     

选择性肠道清洁法对重症烫伤大鼠肠源性内毒素血症的影响

本实验采用大鼠４０％Ⅲ°烫伤模型，初步观察了选择性肠道清洁法（ＳＤＤ）对肠源性内毒素血症的防治效果。结果发现，防治组动物门、体循环内毒素水平伤后均显著降低（Ｐ＜０．０５～０．０１），各段肠腔内游离内毒素含量较烫伤对照组下降９９．５％以上。且肠道细菌易位率致伤１～５天都明显减少，回肠粘膜二胺氧化酶活性逐渐恢复。防治组大鼠严重烫伤５天存活率提高２６．７％（Ｐ＜０．０５）。本结果提示，该方法对于重症烫伤大鼠肠源性内毒素血症具有显著防护效应，并能抑制肠道细菌的移居与减轻肠粘膜的进一步损害。因此，及早进行ＳＤＤ可能有助于烧伤后肠源性感染及其它并发症的防治。     

圆斑蝰蛇毒中性磷脂酶A2对不同种类血管条作用的比较

本文比较了福建圆斑蝰蛇毒中性磷脂酶Ａ２（ＰＬＡ２－３）对人胃网膜动脉条、兔和大鼠主动脉条的作用，表面ＰＬＡ２－３对处于静息状态和预收缩状态的人网膜动脉能诱发松弛反应，而对大鼠和兔主动脉诱发剂量依赖性收缩反应，表明该ＰＬＡ２的血管作用具有血管种类差异性。ＰＬＡ２－３对大鼠主动脉的收缩反应是内皮细胞非依赖性的，去除内皮细胞能增强ＰＬＡ２－３所诱发的收缩反应；ＰＬＡ２－３所诱发的大鼠主动脉条收缩反应可能     

The Turner syndrome research registry: Creating equipoise between investigators and participants

To address knowledge gaps about Turner syndrome (TS) associated disease mechanisms, the Turner Syndrome Society of the United States created the Turner Syndrome Research Registry (TSRR), a patient‐powered registry for girls and women with TS. More than 600 participants, parents or guardians completed a 33‐item foundational survey that included questions about demographics, medical conditions, psychological conditions, sexuality, hormonal therapy, patient and provider knowledge about TS, and patient satisfaction. The TSRR platform is engineered to allow individuals living with rare conditions and investigators to work side‐by‐side. The purpose of this article is to introduce the concept, architecture, and currently available content of the TSRR, in anticipation of inviting proposals to utilize registry resources.     

高中生应对方式及其与学业成绩的关系

目的:探讨高中生面对挫折和烦恼时应对方式的特点及其与学业成绩的关系。方法:以中学生应对方式评定问卷对649名高中生进行了调查。结果:女生比男生更倾向于采用发泄(因子均分2.4±0.7/2.7±0.7,F=23.32,P<0.01)和忍耐(3.2±0.7/3.0±0.8,F=5.08,P<0.05),而男生更倾向于采用幻想(2.4±0.9/2.1±0.8, F=15.52,P<0.01);重点中学比普通中学的高中生更多采用问题解决(3.3±0.6/3.1±0.7,F=20.85,P<0.011幻想(2.4±0.9/2.1±0.9,F=12.87,P<0.01)、退避(2.9±0.6/2.8±0.6,F=7.04,P<0.01)应对;来自城市和农村的高中生应对方式差异不显著;和睦安宁家庭气氛中的高中生采用问题解决应对显著多于偶尔争吵和经常争吵家庭中的高中生;民主型教养方式下的高中生比放任型教养方式的学生更多采用问题解决应对,权威型教养方式家庭中的高中生比民主型教养方式家庭中的高中生更多采用幻想应对。结论:高中生的应对方式存在性别差异。不同家庭气氛和不同教养方式以及学生在学业上的地位对应对方式也有一定影响,但城乡差别不明显。     

^60Coγ射线全身照射后大鼠空肠亮脑啡肽的变化

应用免疫细胞化学(ICC)和放射免疫分析(RIA)方法观察了大鼠4、25Gy~(60)Coγ射线照射后24,48和,72h空肠亮脑啡肽(L-ENK)样免疫反应性神经的分布和L-ENK含量的变化。结果:4Gy照射后24h以及25Gy照射后24,48和72h光镜下见空肠L-ENK样免疫反应性神经的分布密度稍有增加,4Gy照射后24h以及25Gy照射后24,48和72h空肠L-ENK含量分别为29.81±0.84pg/mg,34.96±4.38pg/mg,40.71±3.62pg/mg和38.93±2.31pg/mg,与正常对照组(18.26±1.95pg/mg)相比明显升高(P<9.01)。     

Cytocompatibility of Three Corneal Cell Types with Amniotic Membrane

Rabbit limbal corneal epithelial cells, corneal endothelial cells and keratocytes were cultured on amniotic membrane. Phase contrast microscope examination was performed daily. Histological and scan electron microscopic examinations were carried out to observe the growth, arrangement and adhesion of cultivated cells. Results showed that three corneal cell types seeded on amniotic membrane grew well and had normal cell morphology. Cultured cells attached firmly on the surface of amniotic membrane. Corneal epithelial cells showed singular layer or stratification. Cell boundaries were formed and tightly opposed. Corneal endothelial cells showed cobblestone or polygonal morphologic characteristics that appeared uniform in size. The cellular arrangement was compact. Keratocytes elongated and showed triangle or dendritic morphology with many intercellular joints which could form networks. In conclusion, amniotic membrane has good scaffold property, diffusion effect and compatibility with corneal cells. The basement membrane side of amniotic membrane facilitated the growth of corneal epithelial cells and endothelial cells and cell junctions were tightly developed. The spongy layer of amniotic membrane facilitated the growth of keratocytes and intercellular joints were rich. Amniotic membrane is an ideal biomaterial for layering tissue engineered cornea.