缝隙连接阻断剂对大鼠癫痫后海马涟波振荡能量变化的影响

目的 探讨缝隙连接阻断剂奎宁(quinine,QUIN)、甘珀酸(carbenoxolone,CBX)对癫痫大鼠海马涟波(ripple)振荡能量变化的影响.方法 24只大鼠随机分为模型组、丙戊酸(valproate sodium,VPA)组、QUIN组和CBX组(n=6).建立氯化锂-匹罗卡品(pilocarpine,...     

燃煤型氟中毒大鼠海马区组织病理学分析

目的复制燃煤型氟中毒大鼠模型并观察其对海马CA1区形态学影响。方法24只SD大鼠随机分为3组,对照组、低、高氟组;染氟组以地氟病区燃煤烘烤的玉米为主要饲料,复制氟中毒动物模型,6个月后用氟离子选择电极法检测动物尿、骨及脑氟含量,观察大鼠海马CA1区神经元病理变化,用Biomias 2000图象分析系统测试大鼠海马CA1区神经元胞体平均面积、周长及平均灰度值。结果低、高氟组大鼠尿氟为(2.87±0.12)、(5.27±0.15)mg/L,骨氟(2 912±140)、(4 624±20)mg/kg,脑氟(1.14±0.04)、(1.79±0.04)mg/kg;与对照组尿氟(1.68±0.02)mg/L、骨氟(1479±127)mg/kg、脑氟(0.52±0.05)mg/kg比较,差异有统计学意义(P<0.05或P<0.01);尼氏染色显示,与对照组大鼠神经元平均灰度值(115±4)比较,染氟组大鼠均明显增加[低氟(157±7),高氟(164±8)]。结论长期食用燃煤型氟中毒病区燃煤烘烤的粮食可以引起氟中毒;并导致动物尿、骨、脑氟含量增高,使大鼠海马CA1区神经元蛋白合成功能下降。     

冠脉造影联合动态心电图监测在老年冠心病患者诊断中的应用分析

目的分析冠脉造影联合动态心电图监测在老年冠心病患者患者诊断中的应用价值。方法本次研究选取我院2013年3月至2014年3月治疗的82例冠心病老年患者为例,患者均使用冠脉造影联合12导联动态心电图监测进行诊断。结果 82例患者中,共有63例患者为阳性冠结果,19例患者为阴性结果 ;59例患者12导联动态心电图结果为阳性,23例患者12导联动态心电图结果为阴性。患者冠状动脉病变程度与12导联动态心电图ST低压阳性率差异并不明显,不存在统计学意义(P0.05)。结论只使用12导联动态心电图监测诊断老年冠心病的应用效果较差,可以使用冠脉造影配合诊断,提高老年冠心病的诊断准确率,适合进行临床推广。     

健康人动脉结构及功能改变与室间隔厚度的相关性研究

目的 研究健康人中动脉结构和功能改变与心室间隔厚度的关系.方法 以颈动脉内膜中层厚度( IMT)和肱动脉血流介导的血管舒张反应(FMD)二指标反应动脉结构和功能改变.采用超声多普勒测定68例健康人(男16人,女52人)的IMT、FMD和室间隔厚度( IVST).结果 健康人中IVST与IMT呈正相关(r=0.394,P=-0.001),与FMD呈负相关(r=0.337,P＜ 0.01),IVST也与年龄和收缩压呈正相关.以IVST为因变量的多因素回归分析显示,IMT和FMD是其独立影响因素.结论 健康人中IMT、FMD和IVST相关,动脉结构和功能改变影响心室间隔厚度.     

大蒜素用于非侵袭性真菌性鼻窦炎术后局部冲洗疗效分析

目的评价大蒜素术后局部冲洗对非侵袭性真菌性鼻窦炎的疗效。方法将90例患者随机分为3组,每组30例,分别使用生理盐水、5%碳酸氢钠液+0.1%氟康唑、大蒜素溶液等对非侵袭性真菌性鼻窦炎术后患者进行局部浴洗,随访6个月,比较其有效率。结果大蒜素组与氟康唑组有效率比较差异无统计学意义(P>0.05),但都优于生理盐水组(P<0.05)。结论大蒜素用于非侵袭性真菌性鼻窦炎患者术后局部浴洗有较好疗效。     

Social Media and Internet‐Based Data in Global Systems for Public Health Surveillance: A Systematic Review

Context: The exchange of health information on the Internet has been heralded as an opportunity to improve public health surveillance. In a field that has traditionally relied on an established system of mandatory and voluntary reporting of known infectious diseases by doctors and laboratories to governmental agencies, innovations in social media and so-called user-generated information could lead to faster recognition of cases of infectious disease. More direct access to such data could enable surveillance epidemiologists to detect potential public health threats such as rare, new diseases or early-level warnings for epidemics. But how useful are data from social media and the Internet, and what is the potential to enhance surveillance? The challenges of using these emerging surveillance systems for infectious disease epidemiology, including the specific resources needed, technical requirements, and acceptability to public health practitioners and policymakers, have wide-reaching implications for public health surveillance in the 21st century.Methods: This article divides public health surveillance into indicator-based surveillance and event-based surveillance and provides an overview of each. We did an exhaustive review of published articles indexed in the databases PubMed, Scopus, and Scirus between 1990 and 2011 covering contemporary event-based systems for infectious disease surveillance.Findings: Our literature review uncovered no event-based surveillance systems currently used in national surveillance programs. While much has been done to develop event-based surveillance, the existing systems have limitations. Accordingly, there is a need for further development of automated technologies that monitor health-related information on the Internet, especially to handle large amounts of data and to prevent information overload. The dissemination to health authorities of new information about health events is not always efficient and could be improved. No comprehensive evaluations show whether event-based surveillance systems have been integrated into actual epidemiological work during real-time health events.Conclusions: The acceptability of data from the Internet and social media as a regular part of public health surveillance programs varies and is related to a circular challenge: the willingness to integrate is rooted in a lack of effectiveness studies, yet such effectiveness can be proved only through a structured evaluation of integrated systems. Issues related to changing technical and social paradigms in both individual perceptions of and interactions with personal health data, as well as social media and other data from the Internet, must be further addressed before such information can be integrated into official surveillance systems.     

萘哌地尔对慢性心力衰竭患者神经内分泌的影响

目的 观察萘哌地尔对慢性心力衰竭(CHF)患者神经内分泌的影响.方法 贵阳医学院附属医院2002年3月至2003年5月用放射免疫法测定47例CHF患者(CHF组)与24例健康者(对照组)血肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、内皮素-1(ET-1)、肾上腺髓质素(Adm)、神经肽Y(NPY)、降钙素基因相关肽(CGRP)、A型利钠肽(ANP)、B型利钠肽(BNP)和C型利钠肽(CNP)水平,超声心动图测定左心室射血分数(LVEF).CHF组再随机分为常规治疗组(A组)24例,给予常规心衰治疗;萘哌地尔治疗组(B组)23例,在A组药物治疗基础上加服萘哌地尔,1月后重复上述测定.结果 (1)CHF患者血TNF-α、IL-1、IL-6、ET-1、Adm、NPY、ANP、BNP、CNP水平均显著高于对照组(P＜0.01),而CGRP水平显著低于对照组(P＜0.01).(2)A组和B组治疗前后比较,血TNF-α、IL-1、IL-6、ET-1、Adm、NPY、ANP、BNP、CNP水平均显著降低(P＜0.01),而CGRP水平增高(P＜0.01);(3)B组治疗后与A组治疗后比较,TNF-α、ET-1、ANP、BNP降低更明显(P＜0.05),NPY水平下降更显著(P＜0.01),而CGRP水平升高更明显(P＜0.05),NYHA分级改善更明显(P＜0.05),但IL-1、IL-6、Adm、CNP和LVEF差异无显著性(P＞0.05).(4)LVEF与TNF-α、IL-6、ET-1、BNP呈负相关(P＜0.05).结论 TNF-α、IL-1、IL-6、ET-1、Adm、NPY、CGRP、ANP、BNP和CNP可能与CHF发生发展有关,萘亮哌地尔治疗可能进一步降低CHF患者TNF-α、ET-1、ANP、BNP、NPY水平和提高CGRP水平并改善临床症状.     

Investigation of the sensitivities of distinct gastric cancer cells to parvovirus H‐1 induced cytotoxicity

OBJECTIVE: To investigate the sensitivities of distinct gastric cancer cells to parvovirus H‐1 induced cytotoxicity and the possible mechanism(s). METHODS: There were six distinct differentiated gastric cancer cell lines: HGC27 (undifferentiated), BGC823 (undifferentiated), MKN45 (poorly differentiated), AGS (poorly differentiated), SGC7901 (moderately differentiated) and MKN28 (well differentiated). The cell cycle distributions were measured by flow cytometry and the differential sensitivities of the six distinct gastric cancer cells after H‐1 virus infection were detected by MTT assay. RT‐PCR was used to detect viral NS1 gene expression in all six gastric cancer cell lines. RESULTS: The S phase ratios of HGC27, BGC823, MKN45, AGS, SGC7901 and MKN28 were 24.72%, 30.15%, 27.10%, 29.03%, 31.82% and 33.73%, respectively. HGC27 cells were sensitive to H‐1 virus induced cytotoxicity, followed by SGC7901 cells. MKN45 and AGS cells were moderately sensitive and MKN28 cells were insensitive. However, BGC823 cells were resistant to H‐1 virus induced cytotoxicity. The expressions of viral NS1 were higher in HGC27, BGC823, MKN45 and SGC7901 cells, and lower in AGS and MKN28 cells. CONCLUSIONS: The sensitivities of the distinct gastric cancer cells to H‐1 virus induced cytotoxicity were markedly different. In general, the poorly differentiated cells showed an enhanced sensitivity to H‐1 virus attack compared with well‐differentiated ones. The enhanced sensitivity of poorly versus well‐differentiated gastric cancer cells to H‐1 virus is related in part to the enhanced capacity of the former for NS1 protein production and accumulation. The undifferentiated BGC823 cells were resistant to H‐1 virus triggered cytotoxicity. It may further verify that not all tumor cells are sensitive to H‐1 virus lytic effects.     

扎伊尔型、苏丹型埃博拉病毒TaqMan探针实时定量PCR核酸检测方法的建立

目的建立针对扎伊尔型(ZEBOV)及苏丹型埃博拉病毒(SEBOV)特异、灵敏和快速的Taq Man探针实时定量PCR核酸检测方法。方法选用世界卫生组织(WHO)参比实验室所使用的引物和探针序列,建立针对扎伊尔型及苏丹型埃博拉病毒的NP基因序列保守区的Taq Man探针实时定量PCR核酸检测方法,验证检测方法的灵敏性、特异性和稳定性,并与常规PCR检测方法进行比较。结果所建立的实时定量PCR方法可用于ZEBOV及SEBOV的特异性检测,较传统PCR方法反应快捷、灵敏,分别可达:ZEBOV 34拷贝/反应、SEBOV 24拷贝/反应;具有良好的特异性、稳定性和可重复性。结论建立了ZEBOV及SEBOV Taq Man探针实时定量PCR核酸检测方法,该方法可有望用于埃博拉病毒(EBOV)的实验室、临床及现场核酸检测。     

高浓度尿素诱导人脑微血管内皮细胞系产生炎性因子

目的研究高浓度尿素诱导人脑微血管内皮细胞系(HBMECs)产生炎性因子及其机制。方法以相同渗透压的甘露醇为对照,高浓度尿素(25 mmol/L)干预HBMECs 3、6、12和24 h后,免疫荧光法观察细胞内肿瘤坏死因子α(TNF-α)和诱导型一氧化氮合酶(i NOS)的表达。蛋白免疫印迹法(Western blot)检测TNF-α、i NOS、环氧合酶-2(cycloxygenase-2,COX-2)、核因子κB(NF-κB)/P65和p-P65的表达水平。一氧化氮(NO)试剂盒检测细胞NO含量。结果高浓度尿素增强细胞内TNF-α和i NOS表达。细胞TNF-α、COX-2和p-P65蛋白水平在3和6 h明显高于对照组(P0.01);i NOS蛋白水平持续增高(P0.01)。NO含量在3 h明显增多(P0.05)。结论高浓度尿素诱导人脑微血管内皮细胞产生炎性因子。