节段性肺静脉电隔离术治疗心房颤动的术后护理

心房颤动(房颤)是一种常见的心律失常。随着年龄的增加发生率明显上升,Lasso电极标测下行节段性肺静脉电隔离术是治疗心房颤动最重要的策略之一,因其有明确的消融靶点和消融终点,已成为目前临床应用最广的治疗房颤的技术方法。我院于2001年2月开展此项治疗,效果满意。现将护理总结如下。1一般资料2001年2月—2006年2月接受肺静脉电隔离术治疗心房颤动病人59例,其中男41例,女18例;年龄24岁~75岁,平均45.2岁;持续性房颤20例,阵发性房颤39例。2治疗方法常规穿刺右侧股静脉和左锁骨下静脉,放置冠状静脉窦电极及右心室起搏电极,选用带房间隔穿刺…     

美蓝还原酶法应用于突发水污染微生物指标快速检测的研究

目的 探讨美蓝还原酶法快速检测突发水污染微生物指标即菌落总数的可行性。 方法 通过对美蓝的褪色时间与GB/T5750.12-2006平板计数法的比较,对菌落总数进行检测。 结果 美蓝的还原褪色时间与菌量的多少呈反比,即菌量越大美蓝褪色时间越短。 结论 美蓝还原酶法操作简便,快速,结果准确可靠,可应用于突发水污染的菌落总数的快速检测。     

Aarskog-Scott综合征一例报道

Aarskog-Scott综合征是一种罕见病,典型表现为特殊面容、矮小、性腺发育不良及骨骼畸形等。本文报道1例男性患儿,初次就诊年龄为3岁6个月,主因"发现身高增长缓慢3年"就诊。围生期正常。智力运动发育大致正常。身长90 cm(<第3百分位,-3 SD),特殊面容,关节过伸,双手短粗,脚趾球形。披肩样阴囊,左侧隐睾。FGD1基因第9~12外显子存在1.1 Mb缺失变异,来源于母亲,符合Aarskog-Scott综合征的诊断。患儿自5岁起开始重组人生长激素治疗,治疗18个月,身高增长17 cm,无明显不良反应。当矮小患者同时伴有特殊面容、骨骼畸型及性腺发育不良三联征时,应考虑Aarskog-Scott综合征的可能。     

p38MAPK信号通路与应力介导成肌细胞的凋亡

背景：在体内条件下,细胞力学的功能研究因其所处生理环境的复杂性、实验条件的不易控制而很难得到满意结果。目的：在成功构建成肌细胞体外培养-力学刺激模型的基础上,研究p38MAPK信号通路在成肌细胞凋亡中的作用及其机制。方法：将体外培养的C2C12细胞分为对照组和SB203580组,SB203580组中加入20mmol/L的p38MAPK抑制剂SB203580。应用细胞应力加载装置FlecellStrainUnit-5000T给细胞提供15%的力值,分别施加0,6,12,24h的周期性张应力。每分钟10个循环,每循环包括3s牵张,3s松弛。Hoechst33258染色观察细胞的形态学变化;流式细胞仪检测细胞凋亡情况;RT-PCR法检测促凋亡基因baxmRNA的表达;Westernblot检测信号通路中p38MAPK和p-p38MAPK蛋白的表达。结果与结论：随着加力时间的延长,细胞逐渐出现核固缩及凋亡小体,凋亡率增加（P〈0.05）,baxmRNA表达增多（P〈0.05）;细胞p38MAPK和p-p38MAPK蛋白均在加力6h达到最低,此后逐渐升高。p38MAPK抑制剂SB203580可抑制加力引起的细胞凋亡,减少baxmRNA及p38MAPK和p-p38MAPK蛋白的表达（P〈0.05）。说明p38MAPK信号通路在应力介导的成肌细胞凋亡中起到重要的作用。     

Helicobacter pylori specific immune response induced by conservative flagellin linear B-cell epitope

AIM:To testify the immunogenicity of a conservative B-cell linear epitope of Helicobacter pylori (H pylori) flagellin A. METHODS: Different programs were used to analyze the secondary structure, molecular hydropathy, and surface accessibility of H pylori flagellin A. Linear B-cell epitopes were estimated based on the structural and physiochemical information. Analysis of residue divergence was proposed to screen a conservative linear epitope. The 29-peptide (Pep29mer) synthesized by chemical method, including the predicted conservative B-cell epitope and a known K2d compatible T-cell epitope, was used to immunize mice, and then H pylori-specific antibodies were detected by ELISA. RESULTS: Based on the analyses of divergent amino acid residues, structural and physiochemical characteristics, it was strongly suggested that the short fragment NDSDGR was the core of a conservative linear epitope in flagellin A. Animals immunized by Pep29mer acquired efficient immune response. In detail, serum H pylori-specific IgA and IgGl increased significantly in immunized group, while IgG2a only had an insignificant change. H pylori-specific IgA in gastrointestinal flushing fluid also increased significantly. CONCLUSION: The conservative short fragment NDSDGR is the core of a linear B-cell epitope of flagellin A.     

EGF amplifies the replacement of parvalbumin-expressing striatal interneurons after ischemia

EGF promotes proliferation and migration of stem/progenitor cells in the normal adult brain. The effect of epidermal growth factor on neurogenesis in ischemic brain is unknown, however. Here we show that intraventricular administration of EGF and albumin augments 100-fold neuronal replacement in the injured adult mouse striatum after cerebral ischemia. Newly born immature neurons migrate into the ischemic lesion and differentiate into mature parvalbumin-expressing neurons, replacing more than 20% of the interneurons lost by 13 weeks after ischemia and representing 2% of the total BrdU-labeled cells. These data suggest that administration of EGF and albumin could be used to manipulate endogenous neurogenesis in the injured brain and to promote brain self-repair.     

SARS-CoV S蛋白全长基因克隆及其表达的初步研究

目的:克隆表达SARS-CoVS蛋白,构建SARS全长基因疫苗。方法:从SARS病毒的总cDNA中以PCR方法扩增编码人SARS-CoVS1和S2蛋白的编码序列,再将二者分别克隆入真核表达载体pVAX1,构建重组表达载体。然后进一步将S1和S2连接到pVAX1中。酶切和测序鉴定阳性克隆。采用脂质体法转染VeroE6细胞,用Western检测S蛋白的表达。结果:PCR方法分别扩增出了1900bp和1800bp左右的基因片段,两片段插入pVAX1构建重组表达载体后,经序列测定证实该插入片段为SARS病毒S蛋白编码序列。将重组子转染VeroE6细胞,收集细胞总蛋白,Western检测获得特异蛋白带。结论:成功克隆并表达了SARS-CoVS蛋白,为其作为SARS基因疫苗研究打下基础。     

Serum vascular endothelial growth factor receptor 3 as a potential biomarker in psoriasis

To discover novel biomarkers of psoriasis, a target‐specific antibody array screening of serum samples from psoriasis patients was initially performed. The results revealed that vascular endothelial growth factor receptor 3 (VEGFR‐3) was significantly elevated in the sera of psoriasis patients, compared to healthy controls. Next, ELISA validation studies in a larger cohort of psoriasis patients (N = 73) were conducted, which confirmed that serum VEGFR‐3 was indeed significantly increased in patients with psoriasis compared to healthy controls (P < 0.001). Furthermore, receiver operating characteristic curve analysis demonstrated that serum VEGFR‐3 exhibited potential in distinguishing healthy controls from psoriasis patients: area under the curve = 0.85, P < 0.001. In addition, serum levels of VEGFR‐3 were correlated with Psoriasis Area Severity Index scores (R = 0.32, P = 0.008) in psoriasis patients. Interestingly, serum VEGFR‐3 levels were significantly elevated in psoriatic arthritis compared to non‐psoriatic arthritis (P = 0.026). A pilot longitudinal study demonstrated that serum levels of VEGFR‐3 could reflect disease progression in psoriasis. Collectively, serum VEGFR‐3 may have a clinical value in monitoring disease activity of psoriasis.     

儿童荨麻疹合并肺炎的临床特征及预测指标分析

目的 探讨不同严重程度儿童荨麻疹合并肺炎的临床特征及预测指标分析.方法 选取我院2015年4月—2018年4月收治的荨麻疹合并肺炎患儿54例作为荨麻疹并肺炎组,根据荨麻疹活动评分(UAS)分为轻度组、中重度组.选取同期收治的单纯荨麻疹患儿54例作为单纯荨麻疹组.采血检测患儿的血清总免疫球蛋白E(IgE)、降钙素原(PC...