团体心理干预对大学生自我效能、自我接纳影响的研究

目的:探讨团体心理干预对大学生自我接纳、自我效能水平的影响。方法:将某院校94名1-2年级本科生随机分为实验组(n=47)和对照组(n=47)。实验组实施团体心理干预,每周1次,共10周。结果:①干预前实验组、对照组GSES总分、SAQ总分及因子分无显著差异;②实验组干预后与对照组后测时GSES总分,SAQ总分及自我评价因子分差异显著;③实验组干预前后GSES总分、SAQ总分及自我接纳、自我评价因子分差异显著。结论:团体心理干预能有效提升大学生自我效能、自我接纳水平。     

骨碎补总黄酮可促进兔骨髓间充质干细胞的增殖和分化

背景:骨碎补促进骨损伤愈合疗效确切,但具体作用机制尚不清楚。目的:观察骨碎补总黄酮对兔骨髓间充质干细胞增殖、分化的影响。方法:冲洗兔股骨和胫骨骨髓腔获取骨髓,采用密度梯度离心法联合贴壁培养法体外纯化扩增兔骨髓间充质干细胞;以四甲基偶氮唑蓝法测定不同浓度骨碎总黄酮对兔骨髓间充质干细胞增殖的影响;经骨碎补总黄酮体外诱导后,行扫描电镜形态学观察,碱性磷酸酶染色、茜素红染色和VonKossa银染色了解细胞钙化情况。结果与结论:兔骨髓间充质干细胞可以通过密度梯度离心法联合贴壁培养法扩增和纯化;含骨碎补总黄酮血清培养后,四甲基偶氮唑蓝测定结果显示,骨碎补总黄酮浓度为10-6mmol/L时可明显促进兔骨髓间充质干细胞增殖。经骨碎补总黄酮诱导液诱导,扫描电镜下可见成骨细胞样形态和钙结节形成;细胞碱性磷酸酶染色、钙结节茜素红染色和VonKossa银染色均呈阳性,提示骨碎补总黄酮可促进兔骨髓间充质干细胞增殖和分化。     

Epidemiological investigation on women with uterine prolapse in East Guangdong

目的 调查粤东地区妇女子宫脱垂的流行病学状况,以期了解该病的发生因素.方法 问卷调查120例在门诊就诊的子宫脱垂妇女,同对对既往因子宫脱垂住院手术的80例患者进行问卷调查,将问卷结果进行统计分析.结果 轻度子宫脱垂患者发病年龄在40～45岁者高,占64％,中度子宫脱垂患者在46～50岁者发病率占76％,而重度子宫脱垂患者50岁以上发病率升高,各年龄段的同度子宫脱垂患者比较差异有统计学意义；在体重方面,轻度子宫脱垂患者70％体重指数小于20,30％体重指数正常,与中、重度患者比较差异有统计学意义(P＜0.01),而中度子宫脱垂患者体重指数≤20分别为40％、45％,体重指数大于20占15％；重度子宫脱垂患者70％体重指数大于20.患者均为阴道分娩,结婚年龄均在20～29岁间,无早婚早育史.重度子宫脱垂患者均生育5胎以上.患者均为家庭妇女,月均收入1000～2 000元,平时以蔬菜为主,兼少许鱼类,肉类极少.结论 子宫脱垂早期发病可能与低体重有关,随着年龄、体重增加,重度子宫脱垂人数逐渐增加,多胎多产使子宫脱垂加重.优生优育、产后增强体质、多进食肉类食物等对预防子宫脱垂可能有一定作用.     

Astragalosides IV inhibits high glucose-induced cell apoptosis through HGF activation in cultured human tubular epithelial cells

Astragaloside IV (ASI) in Radix Astragali is believed to be the active component. The study aims to investigate whether ASI inhibits tubular epithelial cells apoptosis induced by high glucose and its mechanisms. Tubular epithelial cells in this paper were isolated from human kidney. The cells apoptosis was detected by TUNEL and caspase 3 assay. The protein levels of HGF and TGF-β1 were measured by ELISA. The phospho-p38 production, ERK and JNK were determined by Western blot. ASI could inhibit cells apoptosis induced by high glucose (25?mmol/L) in dose-dependent and time-dependent manners. ASI also inhibited high glucose-induced expression of TGF-β1 and activation of p38 MAPK pathway at the protein level. Furthermore, ASI increased HGF production in human tubular epithelial cells. The ASI inhibition of tubular epithelial cells apoptosis and reduction of TGF-β1 expression induced by high glucose may represent a new treatment for diabetic kidney injury. The mechanism underlying this inhibitory effect may be related to the inhibition of p38 MAPK signaling pathway activation and HGF overproduction.     

广州市2015-2016年50~74岁社区人群大肠癌筛查结果分析

目的 分析广州市开展现行大肠癌筛查策略的效果。方法 收集、整理2015－2016年广州市大肠癌筛查数据，评价问卷调查、便隐血检查和全结肠镜检查的筛查效果。结果 2015－2016年广州市50～74岁重点人群完成初筛220 834人，初筛阳性37 040例（16.77%）。全结肠镜检7 821人，肠镜检查顺应率为21.12%（7 821/37 040）。发现各类肠道病变4 126例（52.76%），其中进展性腺瘤614例（7.85%）、伴中/重度异型增生的其他病变73例（0.93%），大肠癌230例（2.94%）；各类病变中男性检出率均高于女性（P<0.01）。病变早诊率为87.24%；筛查发现早期癌99例，占总大肠癌的46.26%。大肠癌筛查发现率为104.15/10万，明显高于相应年龄段（50～74岁）大肠癌监测发病率81.18/10万（P<0.001），表现为≤69岁年龄段大肠癌发现率明显高于相应发病率，≥70岁年龄段则相反。结论 广州市大肠癌筛查策略可以发现大肠癌高危人群，提高全结肠镜病变检出率、癌前病变早诊率和早期大肠癌的诊断比例，≤69岁人群受益高于70～74岁人群，是大肠癌筛查重点关注人群，应需进一步促进高危人群肠镜检查顺应性。     

The evaluation of infant stool color card combined with laparoscopy-assisted cholangiography in diagnosis of prolonged jaundice in infants

目的 探讨大便比色卡联合腹腔镜胆管造影在婴儿延迟性黄疸诊断中的价值.方法 72例延迟性黄疸患儿(男34例,女38例,平均日龄64 d),由父母连续7d以上进行大便颜色观察并与比色卡对照:大便颜色持续异常者,以及大便颜色波动在正常与异常之间经内科治疗无效的患儿直接行腹腔镜探查和胆管造影、肝脏组织病理检查;大便颜色持续正常者,以及大便颜色波动在正常与异常之间经内科治疗转为正常的患儿则行内科治疗.所有患儿均行B超、MRCP、ECT检查并随访6～12个月.结果 48例患儿大便颜色连续7d以上为异常,腹腔镜探查及胆管造影结果示:43例为胆道闭锁(BA),5例为非BA.14例患儿大便颜色持续至少7d正常,诊断为婴肝综合征(IHS),内科治疗并随访6～12个月全部治愈.10例患儿大便颜色波动在正常和异常之间,4例经内科治疗后大便颜色转为正常,排除BA行内科治疗,随访3例治愈,1例为漏诊BA;6例内科治疗后无变化,腹腔镜检查诊断1例为IHS,3例为BA,2例为胆道发育不良(CBDH).72例患儿中8例(11.1％)非BA接受了腹腔镜胆管造影,大便比色卡诊断BA的灵敏度、特异度和准确率分别为97.9％、73.9％和90.0％.结论 大便比色卡筛查延迟性黄疸患儿,有选择地行腹腔镜检查,显著降低了IHS患儿行腹腔镜检查比率,提高了BA的早期诊断准确性.     

Mechanistic Insight into Sonoporation with Ultrasound-Stimulated Polymer Microbubbles

Sonoporation is emerging as a feasible, non-viral gene delivery platform for the treatment of cardiovascular disease and cancer. Despite promising results, this approach remains less efficient than viral methods. The objective of this work is to help substantiate the merit of polymeric microbubble sonoporation as a non-viral, localized cell permeation and payload delivery strategy by taking a ground-up approach to elucidating the fundamental mechanisms at play. In this study, we apply simultaneous microscopy of polymeric microbubble sonoporation over its intrinsic biophysical timescales–with sub-microsecond resolution to examine microbubble cavitation and millisecond resolution over several minutes to examine local macromolecule uptake through enhanced endothelial cell membrane permeability–bridging over six orders of magnitude in time. We quantified microbubble behavior and resulting sonoporation thresholds at transmit frequencies of 0.5, 1 and 2 MHz, and determined that sonic cracking is a necessary but insufficient condition to induce sonoporation. Further, sonoporation propensity increases with the extent of sonic cracking, namely, from partial to complete gas escape from the polymeric encapsulation. For the subset that exhibited complete gas escape from sonic cracking, a proportional relationship between the maximum projected gas area and resulting macromolecule uptake was observed. These results have revealed one aspect of polymeric bubble activity on the microsecond time scale that is associated with eliciting sonoporation in adjacent endothelial cells, and contributes toward an understanding of the physical rationale for sonoporation with polymer-encapsulated microbubble contrast agents.