补偿曲线曲度与弦斜度相关性分析

①目的　统一平衡牙合 ５ 个因素的衡量标准平面和单位,探讨弦斜度与补偿曲线曲度的相关关系。②方法　应用 Ｘ 线立体摄影测量结合计算机技术,对哈尔滨市９９ 例青年的补偿曲线进行测量分析,并以圆心角代表补偿曲线曲度。③结果　弦斜度与圆心角呈高度正相关（ｒ＝ ０．９４７１, Ｐ＜ ０．０１）。方差分析示弦斜度性别差异有极显著性（ Ｆ＝ ７．７９１４, Ｐ＜ ０．０１）。④结论　用弦斜度代替补偿曲线曲度是可行的。     

7种化疗药物体外对白血病细胞杀伤作用观察

目的：为了探讨不同化疗药物作用于白血病细胞的特点,为临床化疗提供理论依据。方法：采用溴化四氮唑兰法（ＭＴＴ法）研究了１９例初治急性髓系白血病患有髓原代白血病细胞和Ｋ５６２细胞系体外对７种化疗药物及两药联合物敏感性。结果：柔红霉素（ＤＮＲ）、威猛（ＶＭ２６）,三尖杉酯碱（Ｈ）和米托恩醒（ＭＴＴ）体外杀伤白血病细胞作用较强,其中ＶＭ２６、Ｈ的作用曲线比较独特。两种药物联合使用效果多数按近或好于单用效果     

护士长心理健康水平及其相关因素调查

目的探讨影响护士长心理健康的相关因素。②方法采用症状自评量表（ＳＣＬ－９０）、ＯＬＳＯＮ婚姻质量问卷（ＥＮＲＩＣＨ）和社会支持评定量表等６项心理问卷,对３５０名护士长进行了调查。③结果３５０名护士长的ＳＣＬ－９０中阳性项目数和躯体化因子分、强迫因子分、恐怖因子分、精神病性因子分均显著高于中国常模（ｔ＝２．４８～７．７４,Ｐ＜０．０５,０．０１）。影响护士长心理健康水平的多种因素中以神经质因子、兴奋性因子、敢为性因子、角色平等性因子和婚姻满意度因子的影响较大。④结论人格特征和婚姻质量是影响护士长心理健康的重要因素。     

Does glutathione-S-transferase dethiolate lens protein-thiol mixed disulfides?-A comparative study with thioltransferase.

Protein S-thiolation is a process in which under oxidative stress, vulnerable sulfhydryl groups of proteins are conjugated to non-protein thiols such as glutathione (GSH) or cysteine resulting in the formation of protein-thiol mixed disulfides, protein-S-S-glutathione (PSSG) and protein-S-S-cysteine (PSSC). This process spontaneously disrupts the redox homeostasis of the cells, which in turn leads to functional disturbances in the respective tissue. In the ocular lens, such modification of proteins may trigger a cascade of events starting with the alteration of protein conformation, protein/enzyme deactivation, protein-S-S-protein aggregation and eventually lens opacification or cataract. Generally, the first line of defense system in the cells protects the lens proteins against such damage. Recent studies in our laboratory have shown that in addition to this defense system, lens cells also possess a well developed system to repair the oxidative damage to the lens proteins. We have identified this repair system as thioltransferase (TTase) and have proved that TTase by its dethiolase activity reverses the protein S-thiolation process which returns the oxidatively damaged lens proteins/enzymes to their original reduced state and restores their physiological functions. We investigated if this repair mechanism was mediated by enzymes other than TTase. We studied glutathione S-transferase (GST) and report here for the first time the cloning, high level expression, and purification of human lens mu and pi isoforms of GST. A comparative study of recombinant human lens TTase and GST (mu and pi) on their dethiolating abilities using lens crystallin-thiol mixed disulfides showed that the lens TTase is 60-70% more efficient in the dethiolation/repair process than GST. When TTase and GST were tested in conjunction for the dethiolation of thiol mixed disulfides, there was no significant enhancement of dethiolase activity. These findings suggest that TTase by itself is an efficient enzyme in the dethiolation/repair process and hence can be considered a crucial system to counteract oxidative stress in the lens.     

Probing the binding of indolactam-V to protein kinase C through site-directed mutagenesis and computational docking simulations.

Protein kinase C (PKC) comprises a family of ubiquitous enzymes transducing signals by the lipophilic second messenger sn-1, 2-diacylglycerol (DAG). Teleocidin and its structurally simpler congener indolactam-V (ILV) bind to PKC with high affinity. In this paper, we report our computational docking studies on ILV binding to PKC using an automatic docking computer program, MCDOCK. In addition, we used site-directed mutagenesis to assess the quantitative contribution of crucial residues around the binding site of PKC to the binding affinity of ILV to PKC. On the basis of the docking studies, ILV binds to PKC in its cis-twist conformation and forms a number of optimal hydrogen bond interactions. In addition, the hydrophobic groups in ILV form "specific" hydrophobic interactions with side chains of a number of conserved hydrophobic residues in PKC. The predicted binding mode for ILV is entirely consistent with known structure-activity relationships and with our mutational analysis. Our mutational analysis establishes the quantitative contributions of a number of conserved residues to the binding of PKC to ILV. Taken together, our computational docking simulations and analysis by site-directed mutagenesis provide a clear understanding of the interaction between ILV and PKC and the structural basis for design of novel, high-affinity, and isozyme-selective PKC ligands.     

高效液相色谱法测定美洛昔康胶囊的含量

目的 :建立反相高效液相色谱法测定美洛昔康胶囊中美洛昔康的含量。方法 :采用LichrosorbC18色谱柱 (5 μm,4 .6mm× 2 0 0mm) ,以吡罗昔康为内标 ,甲醇 乙腈 0 .0 9mol·L-1庚烷磺酸钠溶液 冰醋酸 (5 4∶8∶37∶1)为流动相 ,流速为0 .9mL·min-1,检测波长为 35 2nm ,柱温为室温。结果 :美洛昔康在 8～ 4 8mg·L-1范围内呈良好的线性关系 ,r =0 .9999,平均回收率为 99.78% ,RSD为 0 .96 %。结论 :该法简便 ,快速 ,专属性好 ,结果准确 ,可靠。     

氢溴酸右美沙芬片健康人体生物等效性

目的 :研究氢溴酸右美沙芬片在健康人体的药动学及相对生物利用度。方法 :8名健康受试者单剂量随机交叉口服氢溴酸右美沙芬片参比制剂和被试制剂 6 0mg ,采用HPLC法测定用药后不同时间的血药浓度。结果 :2种制剂的体内过程均符合一房室开放模型 ,参比制剂和被试制剂的tmax分别为 (2 72± 0 2 1)h和 (2 74± 0 19)h ,cmax分别为 (5 5 1± 0 4 4) μg·L-1和(5 5 8± 0 2 7) μg·L-1,AUC分别为 (4 5 3± 2 9) μg·h·L-1和 (4 5 7± 3 0 ) μg·h·L-1,被试制剂的相对生物利用度为 (10 1±5 9) %。结论 :2种制剂具有生物等效性。     

高效液相色谱法测定午时茶胶囊中厚朴酚与和厚朴酚含量

目的 :建立中成药午时茶胶囊中厚朴酚与和厚朴酚含量测定的高效液相色谱法。方法 :色谱条件为 AlltimeC1 8(Alltech 5μm,4.6mm× 2 5 mm)柱 ,流动相 :甲醇 -水 (72∶ 2 8) ;流速 :1.0 ml/ min;检测波长 :2 94nm。结果 :测得厚朴酚与和厚朴酚加样回收率为 99.82 % (RSD=0 .89% ,n=6)。结论 :该法可为午时茶胶囊的质量控制和制备工艺提供依据     

针药结合治疗失眠症的临床研究

目的 :观察针药结合与单纯中药治疗失眠症的临床疗效。方法 :90例患者均以国际通用SPIEGEL睡眠量表检测 ,设针药结合组 (治疗组 )与中药组 (对照组 )各 4 5例。结果 :治疗组疗效优于对照组 (P <0 0 5 )。结论 :针药并用 ,内外兼治 ,疗效显著 ,且远期疗效好。