实体语法系统与中医药理论现代化

实体语法系统是一种形式化语法系统，可用于形式化地描述复杂系统的组成单位、组织方式、变化规律。本文尝试将实体语法系统引入中医药理论研究，为实现中医药理论的形式化提供一种新的思路，并在此基础上探讨实现中医药理论与微观领域知识的衔接和用中医药理论解释人体复杂系统的可能性。实体语法系统为中医药理论形式化和中医药理论现代化提供了较具体的研究工具，为中医药理论现代化提出了探索性的思路。     

Efficacy and safety evaluation of human reovirus type 3 in immunocompetent animals: racine and nonhuman primates.

PURPOSE: Human reovirus type 3 has been proposed to kill cancer cells with an activated Ras signaling pathway. The purpose of this study was to investigate the efficacy of reovirus in immunocompetent glioma animal models and safety/toxicity in immunocompetent animals, including nonhuman primates. EXPERIMENTAL DESIGN: Racine glioma cells 9L and RG2 were implanted s.c. or intracranially in Fisher 344 rats with or without reovirus antibodies, followed by treatment of reovirus. To study whether reovirus kills contralateral tumors in the brain and to determine viral distribution, we established an in situ dual tumor model followed by reovirus intratumoral inoculation only into the ipsilateral tumor. To evaluate neurotoxicity/safety of reovirus, Cynomolgus monkeys and immunocompetent rats were given intracranially with reovirus, and pathological examination and/or behavioral studies were done. Viral shedding and clinical biochemistry were systematically studied in monkeys. RESULTS: Intratumorally given reovirus significantly suppressed the growth of both s.c. and intracranially tumors and significantly prolonged survival. The presence of reovirus-neutralizing antibodies did not abort the reovirus' antitumor effect. Reovirus inhibited glioma growth intracranially in the ipsilateral but not the contralateral tumors; viral load in ipsilateral tumors was 15 to 330-fold higher than the contralateral tumors. No encephalitis or behavioral abnormalities were found in monkeys and rats given reovirus intracranially. No treatment-related clinical biochemistry changes or diffuse histopathological abnormality were found in monkeys inoculated intracranially with Good Manufacturing Practice prepared reovirus. Microscopic changes were confined to the region of viral inoculation and were dose related, suggesting reovirus intracranially was well tolerated in nonhuman primates. CONCLUSIONS: These data show the efficacy and safety of reovirus when it is used in the treatment of gliomas in immunocompetent hosts. Inoculation of reovirus into the brain of nonhuman primates did not produce significant toxicities.     

十八甲基炔诺酮透皮控释传递系统在人体不同部位皮肤的药物动力学

6名健康妇女分别于上臂、臀部和腹部三部位经皮给予合LNG的透皮控释传递系统（TCDS）后，用放射免疫法测定LNG血清浓度，计算其主要药物动力学参数。结果表明：在TCDS用药期间，三部位的C（max）、T（max）及AUC（0～168h）基本接近，部位间无显著性差异（P＞0．05）；TCDS揭除后，AUC（168～204h）及消除相半衰期T（1／2）（Ke）均以腹部最大，臀部次之，上臂最小，在腹部与上臂间有显著性差异（P＜0．05）。上述结果可归因于TCDS对LNG的控释和人体皮下脂肪的“贮库效应”。     

肾上腺素诱导兔血小板聚集的实践与理论探讨

目的创建以Ａｄｒ诱导兔血小板聚集的方法，并对受体分子特性作初步探讨。方法以高Ｋ＋缓冲液等取代兔ＰＲＰ中血浆，以Ａｄｒ诱导聚集，以Ａｐｙｒ证实结果。结果兔血小板悬浮于高Ｋ＋缓冲液时Ａｄｒ能单独诱导真正的聚集。结论由此推测血小板膜上α２肾上腺素受体分子可能为由两种亚单位组成：促聚亚单位和辅助亚单位。人辅助亚单位当Ａｄｒ浓度高达聚集阈值以上时可被激活，与促聚亚单位结合为活性的二聚体，与Ａｄｒ进一步结合发生聚集作用。兔辅助亚单位则还需要高Ｋ＋方能被激活     

羊红膻根的化学成分研究

从羊红膻(Pimpinella thellungiana Wolff.)根中分得四个化合物,经理化常数测定和光谱数据解析证明为棕榈酸(Ⅰ)、4-丙烯基苯酚(Ⅱ)、松酯酚(Ⅲ)和新化合物2-甲基-2-羟基-5-甲氧基苯并[d]氢化呋喃-3-酮(Ⅳ)。     

肿瘤靶向基因治疗中基因转移系统的研究

采用同种异体 LAK(Allogeneic-LAK,Allo-LAK)细胞代替患者自身LAK细胞,较好地解决了LAK细胞前体来源问题.通过超声雾化吸入及胸腔内注射Allo-LAK/IL-2治疗肺癌能改善肿瘤微环境,激活肿瘤局部免疫细胞抑制肿瘤细胞,并且较好地解决了Allo-LAK的安全性,避免了大剂量应用IL-2所致的毒副作用.1 材料与方法1.1 病例选择临床确诊为晚期肺癌不能手术切除的14例病人,其中男10例,女4例,年龄39～68岁,平均57.64岁.均经病理或组织细胞学检查确诊为鳞癌7例,腺癌5例,小细胞未分化癌(SCLC)2例,8例并发胸腔积液,4例并发对侧肺转移,3例并发纵隔及锁骨上淋巴结转移,另有2例及1例分别并发骨及肝转移.     

放疗联合化疗治疗复发直肠癌的临床研究

目的探讨放疗联合化疗治疗复发直肠癌的临床效果。方法对２９例根治术后复发的直肠癌进行了放射治疗，其中１６例同时给予大剂量醛氢叶酸（ＣＦ），氟脲嘧啶（５ＦＵ）的全身化疗（观察组），１３例为单纯放疗（对照组）。结果观察组中局部症状缓解率较对照组高，两组有效率分别为８１．２５％、４６．１６％，有显著性差异（Ｐ＜０．０５），完全缓解分别为１８．７５％、７．６９％。两组放疗引起的局部毒性反应相似。结论ＣＦ／５Ｆｕ配合放射治疗复发直肠癌可提高近期疗效，尤其对于伴远外转移患者为理想、安全的治疗手段。     

慢性萎缩性胃炎伴肠化生与胃癌ras基因表达的比较

目的：探讨胃癌发生发展不同阶段癌基因表达特点并予以定量分析，对阐明胃癌发生发展机理及早期诊断胃癌具有重要意义。方法：应用ras基因产物共有多肽序列单克隆抗体PAP免疫组化对照研究了13例慢性萎缩性胃炎伴肠化生（大肠型），32例胃窦癌及15例正常胃窦粘膜ras基因表达特点。结果：慢性萎缩性胃炎伴肠化生粘膜ras基因产物免疫反应阳性细胞形态较规则，呈卵圆形或低柱状，免疫反应强度及阳性细胞数量均较胃癌增强（多）（P均＜0．05）阳性细胞成片分布肠化粘膜下部及基底部，不穿越基底层；胃癌中免疫反应阳性细胞形态不规则，核分裂相明显，成团或散在分布，免疫反应强弱不等，呈异质性表达。正常胃窦粘膜可见谈棕黄色单个分布的ras产物阳性细胞。结论：ras基因在胃癌前病变慢性萎缩性胃炎伴肠化生中已激活，对ras产物精确定量分析有助于胃癌前病变的诊断。     

羊红膻根化学成分的研究Ⅲ.羊红膻素F的分离和鉴定

从草药羊红膻根中又分得2个化合物，经光谱解析（UV，IR，1H、13CNMR，DEPT及1H-1HCOSY，1H－13CCOSY），确定这2个化合物的结构为：4-羟基-丙烯苯-（2-甲基丁酸）酯（Ⅰ），2-羟基-5-甲氧基-丙烯苯-（2-甲基丁酸）酯（Ⅱ）。Ⅰ为新化合物，定名为羊红膻素F。Ⅱ为首次从本植物中分得。