Mesenchymal Stem Cells Display Tumor-Specific Tropism in an RCAS/Ntv-a Glioma Model

Bone marrow-derived mesenchymal stem cells (MSCs) have been shown to localize to gliomas and deliver therapeutic agents. However, the clinical translation of MSCs remains poorly defined because previous studies relied on glioma models with uncertain relevance to human disease, typically xenograft models in immunocompromised mice. To address this shortcoming, we used the RCAS/Ntv-a system, in which endogenous gliomas that recapitulate the tumor and stromal features of human gliomas develop in immunocompetent mice. MSCs were harvested from bonemarrowof Ntv-a mice and injected into the carotid artery of Ntv-a mice previously inoculated with RCAS-PDGF-B and RCAS-IGFBP2 to induce malignant gliomas (n = 9). MSCs were labeled with luciferase for in vivo bioluminescence imaging (BLI). After intra-arterial injection, BLI revealed MSCs in the right frontal lobe in seven of nine mice. At necropsy, gliomas were detected within the right frontal lobe in all these mice, correlating with the location of the MSCs. In the twomice without MSCs based on BLI, no tumor was found, indicating thatMSC localization was tumor specific. In another cohort of mice (n = 9), MSCs were labeled with SP-DiI, a fluorescent vital dye. After intra-arterial injection, fluorescence microscopy revealed SP-DiI-labeled MSCs throughout tumors 1 to 7 days after injection but not in nontumoral areas of the brain. MSCs injected intravenously did not localize to tumors (n = 12). We conclude that syngeneic MSCs are capable of homing to endogenous gliomas in immunocompetent mice. These findings support the use of MSCs as tumor-specific delivery vehicles for treating gliomas.     

Malignant characteristics of circulating tumor cells and corresponding primary tumor in a patient with esophageal squamous cell carcinoma before and after surgery

We report the malignant characteristics of circulating tumor cells (CTCs) and the corresponding molecular features of the primary tumor in a patient with esophageal squamous cell carcinoma (ESCC). A 70-year-old male patient was diagnosed with TNM stage T3N0M0 ESCC. Before surgery, seven intact CTCs and 12 CTCs with a fragmented membrane were detected in 7.5 mL of peripheral blood by immunofluorescence staining. One week after radical resection of the primary tumor, four CTCs were identified in 7.5ml peripheral blood. All CTCs were confirmed as having a malignant phenotype by chromosomal analysis and routine cell staining. Ninety percent of the CTCs were found to have polysomic chromosomes 8 and 20 by fluorescence in situ hybridization (FISH). Immunofluorescence analysis showed that all of the primary tumor cells detected were cytokeratin8/18/19 (CK8/18/19)-positive, but only 1% were CD133-positive. The serum CA19-9 and CEA level were normal in the process of diseases. The patient died 6 months after surgery as a result of lung metastases and other complications. The results of this study suggest that the dynamics and malignant characteristics of both CTCs and the corresponding primary tumor during the disease process may predict tumor burden and the risk of relapse and metastasis.     

肺癌患者的肺康复治疗

肺康复治疗是对有症状、日常生活能力下降的慢性呼吸系统疾病患者采取的一项有循证医学证据、多学科、全面干预的非药物治疗方法。肺康复治疗在肺癌中的应用还处于探索阶段,但初步的临床研究已显示它能有效地改善肺癌患者的运动耐量和生活质量、减少手术并发症并增加手术机会,具有重要的应用前景。     

Inhibition of heme oxygenase-1 enhances the radiosensitivity in human nonsmall cell lung cancer a549 cells

Abstract undergoing radiotherapy or chemotherapy failed to respond. The aim of this study was to evaluate whether Inhibitor of HO-1, zinc protoporphyrin IX (Znpp), enhances the radiosensitivity in human nonsmall cell lung cancer (NSCLC) A549 Cells. A549 cells were induced by Znpp and irradiated by X-rays. Then, expression of HO-1 was measured by real-time polymerase chain reaction. Cell survival was evaluated using the MTS assay and the clonogenic survival assay; apoptosis and cell cycle distribution were monitored by flow cytometry. First, overexpression of the HO-1 mRNA was found in treatment with irradiation alone in A549 cells, and expression of the HO-1 mRNA was reduced after combined treatments with 12?μmol/L of Znpp and irradiation. Second, diminished cell viability percentage, decreased cell clonogenic survival fraction, enhanced cell apoptotic index, and increased percentage of cells in the G1 phase were found after combined treatments with 12?μmol/L of Znpp and irradiation compared to either treatment alone (p<0.05). Inhibitor of HO-1, Znpp, can increase the radiosensitivity of human NSCLC A549 cells.     

CpG oligodeoxynucleotide 1826 enhances the Lewis lung cancer response to radiotherapy in murine tumor

CpG oligodeoxynucleotides (ODNs) are synthetic DNA sequences containing unmethylated cytosine-guanine motifs with potent immune modulatory effects. Via Toll-like receptor 9 agonists of dendritic cells and B cells, CpG ODNs induce cytokines, activate natural killer cells, and elicit vigorous T-cell responses that lead to significant antitumor effects. On the basis of these properties of CpG ODNs, a previous study has tested that they could enhance tumor response to single-dose radiotherapy. The present study extended this finding to the fractionated radiotherapy of the Lewis lung cancer and assessed the ability of CpG ODN 1826 to increase the immune function of mice and the effect of CpG ODN 1826 on the apoptosis of Lewis lung cancer. First, tumor growth delay was observed, and the enhancement ratio of CpG ODN 1826 was found to be 2.4; decreased tumor weight was found after combined treatments with CpG ODN 1826 and X-ray radiation compared with either treatment alone (P < 0.01). Second, enhanced cell apoptotic index was found after combined treatments with CpG ODN 1826 and X-ray radiation compared with either treatment alone (p < 0.01). Increased tumor necrosis factor-α and decreased interleukin-10 concentration in serum and enhanced spleen exponent were observed after combined treatments with CpG ODN 1826 and X-ray radiation compared with X-ray radiation treatment alone (p < 0.01). Results suggest that CpG-ODN1826 can increase the radiosensitivity of Lewis lung cancer, which may be associated with stimulation of immune system and enhanced cell apoptosis.     

ACS患者与稳定性心绞痛患者血清抵抗素水平对比研究

目的:探讨ACS患者与稳定性心绞痛患者血清抵抗素水平差异及其临床意义.方法:研究对象138例,经冠状动脉造影证实,稳定型心绞痛(SAP)71例,急性冠脉综合征(ACS)67例.应用ELISA方法测定各组空腹血清抵抗素、高敏C反应蛋白(hs-CRP).结果:ACS组血清抵抗素(6.06±0.80 ms/L)及高敏C反应蛋白(19.42±1.51 mg/L)与SAP组(2.87±0.78 mg/L、10.32±1.00)相比均增高(P<0.01).冠心病患者血清抵抗素与hs-CRP呈正相关(r=0.798,P=0.006).结论:提示血清抵抗素可能通过恶化全身炎症水平,在ACS的发病中起作用.     

Ginsenoside Rg1 restores the impairment of learning induced by chronic morphine administration in rats

Rg1, as a ginsenoside extracted from Panax ginseng, could ameliorate spatial learning impairment. Previous studies have demonstrated that Rg1 might be a useful agent for the prevention and treatment of the adverse effects of morphine. The aim of this study was to investigate the effect of Rg1 on learning impairment by chronic morphine administration and the mechanism responsible for this effect. Male rats were subcutaneously injected with morphine (10 mg/kg) twice a day at 12 hour intervals for 10 days, and Rg1 (30 mg/kg) was intraperitoneally injected 2 hours after the second injection of morphine once a day for 10 days. Spatial learning capacity was assessed in the Morris water maze. The results showed that rats treated with Morphine/Rg1 decreased escape latency and increased the time spent in platform quadrant and entering frequency. By implantation of electrodes and electrophysiological recording in vivo, the results showed that Rg1 restored the long-term potentiation (LTP) impaired by morphine in both freely moving and anaesthetised rats. The electrophysiological recording in vitro showed that Rg1 restored the LTP in slices from the rats treated with morphine, but not changed LTP in the slices from normal saline- or morphine/Rg1-treated rats; this restoration could be inhibited by N-methyl-D-aspartate (NMDA) receptor antagonist MK801. We conclude that Rg1 may significantly improve the spatial learning capacity impaired by chonic morphine administration and restore the morphine-inhibited LTP. This effect is NMDA receptor dependent.     

中等卫生职业学校班主任应当重视培养学生的团队精神

现阐述中等卫生职业学校的班主任通过树立集体的共同目标,培养学生的团结协作精神、宽容与合作的品质,充分展示学生的个性来培养学生的团队精神。     

飞行时间质谱技术在肾脏缺血/再灌注(I/R)损伤研究中的应用

目的 应用MALDI-TOF MS技术检测和探讨肾脏缺血/再灌注(I/R)损伤大鼠血清中蛋白质组学的变化.方法 制备大鼠肾脏I/R模型后,分别于再灌注后6、12、24h选取各组血清样本,经MALDI-TOF MS分析检测,并对各组具有差异的多肽指纹图谱鉴定分析.用SPSS13.0软件对数据进行处理.同时采用 Mascot Search进行蛋白质数据库检索以确定其性质.结果 ①本研究中血清经IMAC-Cu bead 处理后,在m/z为2081Da处,检测得到具有统计学意义的多肽指纹图谱;②采用 Mascot Search,进行了蛋白质数据库检索, 均鉴定为大鼠纤维蛋白原片段.结论 纤维蛋白原在肾脏缺血/再灌注损伤中可能起重要作用.