非断离腹壁韧带腹膜外剖宫产术式探讨

行腹膜外剖宫产术时,在侧脐韧带与中脐韧带之间进行操作,不切断腹壁任何韧带,用撕拉、钝性剥离的方法分离膀胱与腹膜,子宫切口连续缝合两层,并将宫颈前筋膜与子宫切口浅层一并连续缝合.该方法不仅缩短了手术时间,避免了新生儿Apgar's低评分、新生儿窒息的发生,而且维持了腹壁结构的完整性,可进一步降低术后各种近、远期并发症的发生率.     

A retrospective study of neck rejuvenation using a noninsulated microneedle radiofrequency in Chinese subjects

Lasers in Medical Science - Large-scale long-term retrospective study of noninsulated microneedle radiofrequency treatment on neck rejuvenation is lacking. We conducted a 3-year retrospective...     

中医外治法在慢性萎缩性胃炎中的应用研究进展

慢性萎缩性胃炎（CAG）是消化系统常见的慢性疾病,被认为是胃癌癌前病变,严重危害人类健康。如何有效促进胃黏膜的修复、抑制腺体的萎缩、阻断癌变是临床研究的热点。目前西医尚缺乏特效的治疗手段,近年来,大量研究结果表明,中医外治法在治疗CAG方面取得了显著成果,且具有简、便、效、廉等优势,深受患者青睐。现查阅近年来包括针刺法、灸法及贴敷法等在内的常用中医外治法治疗CAG的相关文献并进行综述,以期为临床治疗该病提供参考。     

Microiontophoresis of cocaine, desipramine, sulpiride, methysergide, and naloxone in habenula and parafasciculus

The effects of microiontophoretically applied cocaine, desipramine (DES), sulpiride (SUL), methysergide (METH), and naloxone (NAL) on the responses of physiologically identified single neurons in the habenula (Hab) and parafasciculus thalami nucleus (PF) were examined in rats. Three cell types were identified in both nuclei on the basis of the responses obtained following noxious stimulation that were classified as "nociceptive-on," "nociceptive-off," and "nonnociceptive" cells. Administration of cocaine generally resulted in a decrease in the firing rate of nociceptive-on and nonnociceptive neurons in both Hab and PF. In contrast, cocaine generally induced an excitation in the baseline firing of the nociceptive-off cells. Cocaine application concomitant with noxious stimulation prevented the evoked responses of the nociceptive-on and the nociceptive-off cells. DES, when applied alone, was found to induce excitation in neuronal discharge of all three cell types in both sites. Combined application of cocaine with DES resulted in no observable change in discharge frequency for the nociceptive-on and nonnociceptive cells, while inducing an additive excitatory effect on the nociceptive-off cells. SUL, in contrast, induced no observable effect on baseline firing when given alone, yet consistently antagonized cocaine-induced effects on all three cell types. Finally, METH and NAL induced no effects on baseline firing or cocaine-induced modifications in neuronal discharge frequency.     

In vitro assembly of infectious nucleocapsids of bacteriophage phi 6: formation of a recombinant double-stranded RNA virus.

A system is described for assembling infectious bacteriophage phi 6 nucleocapsids in vitro. Procapsids encoded by cDNA copies of genomic segment L in Escherichia coli were used to package and replicate viral RNA segments. The resulting filled particles were shown to be capable of infecting host cell spheroplasts after incubation with purified nucleocapsid shell protein P8. The infected spheroplasts yielded infectious virions. A modified cDNA-derived RNA segment was inserted into virions by this method. The resulting infectious virions contained the same 4-base-pair deletion as the modified cDNA. These findings support the contention that the preformed procapsids are the "machine" that replicates the phi 6 genome, by showing that the cDNA-derived procapsids are competent to package and replicate RNA properly.     

偶联剂CGE（N）—R型处理前后牙本质的表面状态及其...

In order to research the states of dentine surface and dentine surface primed with coupling agent, we used microscope, transmission electron microscope (TEM) and solid fluorescence method. Observed under microscope, patterning of dentinal tubules and their poles was very explicit; through the view of interface between coupling agent adsorbed and human dentine powder by TEM, it was revealed that the coupling agent was adsorbed onto the dentine surface as if it "grew out" of the dentine surface; through examination of interface between the coupling agent adsorbed and dentine surface by fluorescence spectrum, which differed from that of the coupling agent or dentine surface, i.e., this fluorescence spectrum of interface changed obviously (red shift). This phenomenon indicated that there was a chemical reaction occurring between tooth surface and adsorbed coupling agent. Therefore we can conclude that the three examinations are necessary to provide the argument for establishment of chemical bonding mechanism between dentine and coupling agent.     

Determination of trace selenium in human body by anodic stripping voltammetry at gold-disc electrode

Summary For determination of trace Se we used high-pressure wet digester to pretreat the specimens obtained from the human body. This method is very effective in preventing loss of Se by volatilization which often occurs in the sample pretreatment. We studied the method of using anodic stripping voltammetry at gold-disc electrode to measure the trace Se present in the human body and found it to be the optimal procedure. The detection limitation was about 2x10⁻⁸ g/L, the recovery rate of Se about 96.6±6,7 % , and the coefficient of variation CV = 7.0%.     

5-氨基水杨酸灌肠对大鼠免疫性结肠炎的影响

目的 探讨5-氨基水杨酸(5-ASA)灌肠对大鼠三硝基苯磺酸(TNBS)诱导的结肠炎的作用.方法用TNBS诱发大鼠结肠炎.造模7天后,用不同剂量的5-ASA(25、50、100mg·kg-1·d-1)开始灌肠.观察大鼠粪隐血(OB)反应强度、结肠大体形态和组织学改变,并检测肠黏膜髓过氧化物酶(MPO)活性.结果 5-ASA灌肠可明显降低结肠黏膜损伤指数(CMDI)、OB反应强度、MPO活性及组织学评分(HS),各剂量组间有一定的量效关系.结论 5-ASA灌肠对TNBS诱导的大鼠结肠炎有保护作用,且与剂量有一定关系.     

提高大脑皮质神经元细胞产率以及活性的研究

目的　提高培养大脑皮质神经元的产率以及活性 ,使实验的细胞模型有良好的一致性和可比性。方法　实验分析了大脑皮质神经元培养过程中 ,从选材、取材到分散细胞等一系列步骤中维持细胞活性的内在因素 ,应用细胞形态学指标 ,Trypanblue染色计数分析以及染色观察。结果　每侧大脑皮质得到细胞约 2 .5× 10 6～ 3.0× 10 6个 ,而且种植不同时间 6 ,12 ,48h细胞存活率均在 95 %以上。结论　该法是较好的大脑皮质神经元培养方法。