Fatigue in early Parkinson’s disease

Objective To investigate the prevalence,character-istics and risk factors of fatigue in a large cohort of subjects with early Parkinson’s disease (PD).Methods A total of 391 individuals with PD,recrutied in Linzhi trial, were re-screened. Early, non-depressed subjects     

P-糖蛋白抑制剂对两性霉素B血脑屏障转运的作用

目的 探讨P-糖蛋白抑制剂对两性霉素B(AmB)血脑屏障转运的影响.方法 应用小鼠脑血管内皮细胞建立体外血脑屏障模型,选取维拉帕米作为P-糖蛋白抑制剂,AmB作为受试底物,经细胞毒性实验选定合适的温育时间及药物浓度,分别进行不同时间点的AmB细胞摄取实验及不同维拉帕米浓度的AmB细胞摄取实验.组间均数比较采用单因素方差分析,均数两两比较采用Bonferroni检验.结果 AmB的血脑屏障转运随时间延长不断积累,与同一时间点对照组相比,抑制剂组在90 min(t=6.753,P=0.001)、120 min(t=3.574,P=0.016)、150 min(t=4.759,P=0.005)处AmB细胞摄取水平明显升高,其余时间点两组间差异无统计学意义.与阴性对照组相比,AmB细胞摄取水平在不同浓度维拉帕米组(2、5、10、50、75和100μmol/L)均有显著提高(P=0.000、0.014、0.000、0.014、0.000、0.000),且随维拉帕米浓度升高增加幅度更明显.结论 P-糖蛋白抑制剂维拉帕米可提高脑毛细血管内皮细胞对AmB的摄取作用,提示P-糖蛋白参与血脑屏障AmB的转运.     

驱白巴布期片与高能紫外光联合治疗白癜风疗效观察

目的：观察驱白巴布期片与高能紫外光联合治疗白癜风的疗效。方法：将符合入选标准的108例门诊患者随机分为3组：治疗组：口服驱白巴布期片,3片／次,3次／日,配合高能紫外光照射,每周1次;对照1组：用高能紫外光照射,每周1次;对照2组：予驱白巴布期片内服,3片／次,3次／日。疗程均为3个月。结果：治疗组疗效明显优于对照1组和对照2组,疗效比较x2值分别为5．509和5．940,P〈0．05。结论：驱白巴布期片与高能紫外光联合治疗白癜风疗效好。     

如何切实有效地开展胃肠肿瘤的转化医学研究

转化医学作为医学发展的新理念,虽然定义尚未明确,但其作为连接临床医学和实验室之间的桥梁,重要性已日益凸显。胃肠外科的发展始终体现着转化医学的理念。为开展转化医学研究,胃肠外科医师必须学习从临床中寻找研究问题,完整收集临床资料,重视团队协作,特别是跨学科的合作,同时充分利用各种资源。通过转化医学研究,胃肠外科医师能够进一步提高我国胃肠道肿瘤的综合诊治水平,提高患者长期生存率。     

Comparative characterization of stem cells from human exfoliated deciduous teeth and dental pulp stem cells

ObjectiveThis study focused on the characterization of stem cells from human exfoliated deciduous teeth (SHED) in comparison with dental pulp stem cells (DPSCs) to certify SHED as a key element in tissue engineering.MethodsIn the present study, SHED and DPSCs were assayed for their cell surface antigens and proliferation by measuring the cell cycles, growth rates, Ki67-positive efficiencies, and colony-forming units (CFUs). The evaluation of multi-differentiation was performed using alizarin red and oil red O and real-time PCR in vitro. The mineralization capability of the cells was examined in vivo by implanting with ceramic bovine bone (CBB) into subcutaneous of immunocompromised mice for 8 weeks. A three-dimensional pellet cultivation system is proposed for SHED and DPSCs to recreate the biological microenvironment that is similar to that of a regenerative milieu.ResultsSHED showed a higher proliferation rate and differentiation capability in comparison with DPSCs in vitro, and the results of the in vivo transplantation suggest that SHED have a higher capability of mineralization than the DPSCs. The mRNA expression levels of inflammatory cytokines, including matrix metalloproteinase-1 (MMP1), tissue inhibitors of metalloproteinase-1 (TIMP1), matrix metalloproteinase-2 (MMP2), tissue inhibitors of metalloproteinase-2 (TIMP2) and interleukin-6 (IL-6) were higher in SHED than that in DPSCs. In addition, the expression levels of Col I and proliferating cell nuclear antigen (PCNA) in SHED sheets were significantly higher than those in DPSCs sheets.ConclusionsThis study systematically demonstrated the differences in the growth and differentiation characteristics between SHED and DPSCs. Consequently, SHED may represent a suitable, accessible and potential alternative source for regenerative medicine and therapeutic applications.     

Genetic association study of glucocerebrosidase gene L444P mutation in essential tremor and multiple system atrophy in mainland China

The glucocerebrosidase (GBA) gene mutation is emerging as an important risk factor for Parkinson’s disease. We previously reported that the GBA gene L444P mutation is an important risk factor for PD in the Chinese population. The prevalence of this mutation in other neurodegenerative diseases and movement disorders remains completely unexplored in mainland China. In the present study, we extended the screening of GBA gene L444P mutation to Chinese patients with essential tremor (ET) and multiple system atrophy (MSA). We searched for the GBA gene L444P mutation in 109 patients with ET, 54 patients with MSA, and 657 controls from mainland China. None of the 109 patients with ET or 54 patients with MSA carried the GBA gene L444P mutation. Among the 657 controls, we found one L444P heterozygote. The difference in mutation frequencies between patients with ET or MSA and the control group was not statistically significant (chi-squared test, p = 1, respectively). The results suggest that the GBA gene L444P mutation may be not responsible for ET in mainland China. Whether the GBA gene L444P mutation modifies the risk for MSA deserves further study in larger samples.