柴胡与狭叶柴胡的特异性引物PCR鉴别方法

目的设计专用于柴胡与狭叶柴胡鉴别的ARMS特异性引物,为柴胡、狭叶柴胡的药材鉴别提供分子鉴定依据。方法根据柴胡、狭叶柴胡与其他柴胡属植物的r DNA ITS序列,寻找柴胡与狭叶柴胡的序列特异性位点,设计用于进行柴胡与狭叶柴胡分子鉴别的特异性引物。结果该特异性引物为柴胡、狭叶柴胡的鉴别提供了分子鉴定依据,可从多种药材及中成药(蜜丸、水蜜丸)中快速、准确地检测出柴胡、狭叶柴胡。结论运用特异性引物PCR法能有效地从植物和中成药(蜜丸及水丸)中鉴别出柴胡与狭叶柴胡,该方法具有准确、高效、省时、简便的特点。     

基于HPLC指纹图谱的浙贝母质量评价

目的:建立浙贝母药材HPLC指纹图谱,为其质量控制提供比较全面的评价方法。方法:Welch Xtimate^TM C₁₈色谱柱(4.6 mm×250 mm,5 μm);流动相乙腈-10 mmol·L^-1乙酸铵溶液(氨水调pH至10.0),梯度洗脱;流速0.8 mL·min^-1,柱温25℃,蒸发光散射检测器。利用化学计量学方法,包括相似度分析、聚类分析、主成分分析,对色谱数据进行分析。结果:该方法精密度、稳定性、重复性良好。测定了16批浙贝母药材,提取7个色谱峰作为指纹图谱共有峰,采用相似度评价、聚类分析和主成分分析等方法,对所收集的16批样品进行系统比较与归类,16批样品分为2类。结论:该法重复性好,简便可靠,可以为浙贝母的质量控制和评价提供依据。     

Abnormal inter- and intra-hemispheric integration in male paranoid schizophrenia: a graph-theoretical analysis

Background

The human brain is a complex network of regions that are structurally interconnected by white matter (WM) tracts. Schizophrenia (SZ) can be conceptualized as a disconnection syndrome characterized by widespread disconnections in WM pathways.

Aims

To assess whether or not anatomical disconnections are associated with disruption of the topological properties of inter- and intra-hemispheric networks in SZ.

Methods

We acquired the diffusion tensor imaging data from 24 male patients with paranoid SZ during an acute phase of their illness and from 24 healthy age-matched male controls. The brain FA-weighted (fractional anisotropy-weighted) structural networks were constructed and the inter- and intra-hemispheric integration was assessed by estimating the average characteristic path lengths (CPLs) between and within the left and right hemisphere networks.

Results

The mean CPLs for all 18 inter-and intra-hemispheric CPLs assessed were longer in the SZ patient group than in the control group, but only some of these differences were significantly different: the CPLs for the overall inter-hemispheric and the left and right intra-hemispheric networks; the CPLs for the interhemisphere subnetworks of the frontal lobes, temporal lobes, and subcortical structures; and the CPL for the intra- frontal subnetwork in the right hemisphere. Among the 24 patients, the CPL of the inter-frontal subnetwork was positively associated with negative symptom severity, but this was the only significant result among 72 assessed correlations, so it may be a statistical artifact.

Conclusions

Our findings suggest that the integrity of intra- and inter-hemispheric WM tracts is disrupted in males with paranoid SZ, supporting the brain network disconnection model (i.e., the ^‘connectivity hypothesis^’) of schizophrenia. Larger studies with less narrowly defined samples of individuals with schizophrenia are needed to confirm these results.     

血管封堵器在心导管术后的应用

血管封堵器被广泛应用于心导管术后股动脉穿刺部位止血。与传统的手工压迫止血相比 ,血管封堵器止血更安全有效 ,缩短患者卧床时间。本文比较了几种不同类型的血管封堵器的特点 ,供临床参考。     

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??OBJECTIVE To understand the application of warfarin hospitalized patients with non-valvular atrial fibrillation, summarize the clinical experience and provide reference for clinical medication, and explore the significance of clinical pharmaceutical care for warfarin. METHODS The information of 726 cases with non-valvular atrial fibrillation hospitalized in the first cardiovascular ward from January 2012 to December 2013 were retrospectively collected and analyzed in the aspect of types of anticoagulant solution, warfarin dosage and INR monitoring. RESULTS The anticoagulation solution commonly used in our patients with atrial fibrillation included dual antiplatelet therapy, aspirin antithrombotic therapy and warfarin anticoagulant therapy. 13.8% of the cases had initial INR of 2.0-3.0, and 43.4% of the cases attained INR target after dosage adjustment. CONCLUSION At present the clinical application of warfarin still exists deficiencies. It is necessary to continue to raise people??s awareness of warfarin and atrial fibrillation anticoagulant. Active clinical pharmaceutical care for warfarin is of great significance.
     

不同方法对食源性致病菌耐药性监测研究

目的了解江苏地区食源性致病菌的常用抗菌药耐药谱,评价不同药敏方法在致病菌耐药性监测中的适用性。方法用Etest和微量肉汤稀释法,对江苏省2012-2014年食源性疾病常规监测检出的金黄色葡萄球菌、副溶血性弧菌、沙门菌菌株进行药敏鉴定。结果金黄色葡萄球菌对红霉素的耐药率最高,其次为复方磺胺甲噁唑和四环素,且有多重耐药;副溶血性弧菌除对四环素和复方磺胺甲噁唑有一定的耐药,对其他6种革兰氏阴性菌抗生素均敏感。沙门菌对萘啶酸的耐药率超过50.0%。两种方法药敏检测结果对不同抗生素符合率有差异,但符合率均80%。结论 Etest和微量肉汤稀释法有较好的一致性,但所需时间及操作要求有差异,可根据实际需要进行选择。     

慢性肾衰竭血液透析患者的胃排空

目的进一步明确慢性肾衰竭血液透析患者的胃排空是否存在异常。方法采用磁示踪法对32例慢性肾衰竭血液透析患者的固相胃排空时间进行了测定,并与正常对照组进行了对比。结果 透析后的胃半排空时间与正常对照组比较,二者差别无显著的统计学意义(P>0.05)。结论 慢性肾衰竭血液透析患者胃排空时间是正常的。     

控制图原理及在传染病预警中的应用现状

在传染病发生的早期阶段做出预警,可及时采取干预措施,尽可能降低传染病对人类健康和对社会经济、政治等的影响.控制图法是一种简单易行且功效高的预警技术,但也有不足之处.     

黄芪总黄酮联合顺铂对Lewis荷瘤小鼠抗癌作用的研究

本研究旨在分析黄芪总黄酮(total flavonoids of Astragalus membranaceus, TFA)联合顺铂对Lewis荷瘤小鼠的抗癌作用及机制,动物实验获得山西大学伦理委员会的批准(SXULL2018012)。肿瘤重量、肿瘤体积变化曲线、抑瘤率等药效学指标以及脏器指数分析显示黄芪总黄酮对顺铂的抗肿瘤作用具有增效减毒作用;进一步采用网络药理学技术预测黄芪总黄酮的作用靶点,结果显示毛蕊异黄酮葡萄糖苷可能是抑制肺癌的主要活性成分,细胞肿瘤抗原p53 (TRP53)、与Ras相关的C3肉毒毒素底物1 (RAC1)、受体酪氨酸蛋白激酶erbB-2 (ERBB2)、血管内皮生长因子A (VEGFA)、信号转导子和转录激活子3 (STAT3)等靶点可能与黄芪总黄酮对顺铂的增效减毒作用相关;血清IL-6含量以及肿瘤组织STAT3、p53蛋白的表达水平结果显示,黄芪总黄酮可能通过IL-6/STAT3通路来抑制肿瘤生长; UPLC-MS血清代谢组学分析结果提示,与肺癌相关的代谢通路包括鞘脂代谢通路、视黄醇代谢通路、甘油磷酸代谢通路、原代胆汁酸生物合成通路,黄芪总黄酮可回调成分相对...     

Isolation and Characterization of Two New Deoxynivalenol-Degrading Strains,Bacillus sp. HN117 and Bacillus sp. N22

Deoxynivalenol (DON), produced by Fusarium species, is one of the most common trichothecenes detected in cereals pre- and post-harvest, which poses a great threat to the health of livestock and human beings due to its strong toxicity. In this study, we isolated and characterized two DON-degrading bacterial strains, Bacillus sp. HN117 and Bacillus sp. N22. Both strains could degrade DON efficiently in a wide range of temperatures (from 25 °C to 42 °C) and concentrations (from 10 mg/L to 500 mg/L). After optimization of the degradation conditions, 29.0% DON was eliminated by HN117 in 72 h when it was incubated with 1000 mg/L DON; meanwhile, the DON degradation rate of N22 was boosted notably from 7.41% to 21.21% within 120 h at 500 mg/L DON. Degradation products analysis indicated HN117 was able to transform DON into a new isomer M-DOM, the possible structure of which was deduced based on LC-MS and NMR analysis, and N22 could convert DON into potential low-toxic derivatives norDON E and 9-hydroxymethyl DON lactone. These two strains have the potential to be developed as new biodegrading agents to control DON contamination in food and feed industries.