去甲长春花碱加顺铂联合治疗晚期非小细胞肺癌近期疗效分析

目的:观察国产去甲长春花碱(NVB)加顺铂(DDP)联合治疗晚期非小细胞肺癌的疗效.方法:符合入选条件的ⅢB-Ⅳ期NSCLC患者48例,治疗方案NVB 25mg/m2,iv dl,8,DDP 20mg/m2,iv dl-5,21天为一周期,每3周重复一次,每例至少用2个周期.结果:PR 20例,SD 20例,PD 8例,有效率44%,中位生存期10个月.主要毒副反应为骨髓抑制,轻度的胃肠道反应及少数局部静脉炎.结论:国产去甲长春碱加顺铂联合化疗治疗晚期非小细胞肺癌疗效明显,毒副反应可耐受.     

不同起搏模式对病人生存质量影响的调查研究

[目的]探讨不同起搏模式对病人生存质量（QOL）的影响。[方法]以健康调查问卷（SF一36量表）对112例不同起搏模式病人进行问卷调查，比较3种起搏模式下病人的QOL水平。[结果]除机体疼痛、角色情绪2个维度外其余6个维度（生理功能、角色限制、活力、社会功能、精神健康、总体健康）3种起搏模式间比较差异有统计学意义（P〈0．05）。[结论]应用AAI型、DDD型起搏器的病人生存质量优于VVI型起搏。     

替牙(牙合)时期前牙反(牙合)矫治中弓丝弯制的探讨

目的　探讨在“2× 4”矫治技术中弓丝弯制的方法及原理。方法　替牙牙合时期前牙反牙合患者 2 4例 ,使用粗弓丝 ,在其弓丝上弯制各种曲形 ,并根据不同错牙合类型 ,处理各种曲与牙齿、带环关系 ,防止出现磨牙旋转移动等异常。结果　 2～ 6个月纠正反牙合 ,2 4例替牙牙合时期反牙合患者得以早期矫治。结论　本方法矫治时间短 ,效果明显 ,操作简单 ,能够达到较为理想的矫治效果     

持续压力超负荷对兔左心室细胞凋亡的影响

OBJECTIVE: To study the effect of chronic pressure overload on the apoptosis of the left ventricle myocytes in rabbits. METHODS: Rabbit models of chronic pressure overload-induced heart failure were prepared in which dynamic changes of apoptotic myocytes in the left ventricle were observed by way of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay. RESULTS: Only a few apoptotic cells was observed in the sham-operated group, while in the experimental group, the apoptotic left ventricle myocytes significantly increased after operation, presenting a peak level between day 3 and day 7. Seven days after the operation, the apoptotic myocytes began to decrease and till day 14, the apoptotic cell number had been smaller than that measured on day 1. When signs of heart failure set in, the apoptotic myocytes were again increased (P<0.001). CONCLUSION: During chronic pressure overload, myocyte apoptosis in the left ventricle is elevated at the early stages and undulates subsequently, with the peak occurring before hypertrophy is obvious.     

Post-translational processing of thyrotropin-releasing hormone precursor in rat brain: identification of 3 novel peptides derived from pro TRH

The sequence of rat hypothalamic pro-thyrotropin releasing hormone, deduced by sequencing of cDNA, in addition to 5 TRH progenitor genitor sequences contains leader, trailer and 4 intervening sequences separated by paired basic amino acid sequences. We have developed radioimmunoassays to synthetic peptides corresponding to portions of these cryptic proTRH sequences and have used these assays to identify and partially characterize proTRH peptides, distinct from TRH, in extracts of rat brain. Two of these peptides correspond closely in size to one intervening sequence and the car☐y-terminal sequence of proTRH. Three other peptides correspond to the intact amino-terminal leader sequence and two peptides formed by a further cleavage of the leader sequence at an internal paired basic amino acid sequence.     

内分泌激素与脑血管疾病的相关性研究进展

众多的激素参与脑血管疾病的病理过程 ,与其发展预后密切相关。内分泌激素的研究为脑血管疾病的预后和治疗提供广阔的前景 ,如果能进一步研究脑血管疾病和激素的关系 ,必然会使脑血管疾病预后的判断和治疗跨上一个新台阶 ,为激素受体拮抗剂的临床应用开拓新天地。     

侧脑室注射东莨菪碱和酚妥拉明对幼兔分辨学习的影响

目的 探讨东莨菪碱和酚妥拉明对幼兔分辨学习的影响。方法 将30只比利时纯种幼兔从生后13日龄随机分为三组：东莨菪碱组、酚妥拉明组和生理盐水对照组，观察不同日龄各组幼兔的分辨学习能力。结果 幼兔分辨学习的正确反应一般始于生后15日龄。侧脑室注射东莨菪碱对16日龄以前幼兔分辨学习无明显影响，对17日龄以后幼兔分辨学习产生明显抑制作用。侧脑室注射酚妥拉明对13—23日龄幼兔分辨学习均有明显抑制作用。结论 幼兔生后发育过程中学习能力的发展具明显的阶段性，其原因可能与神经递质系统的发育成熟有关。     

胰岛素样生长因子-Ⅰ对软骨细胞凋亡的抑制

目的:探讨胰岛素样生长因子-Ⅰ(IGF-Ⅰ)对软骨细胞增殖及白细胞介素-1(IL-1)诱导软骨细胞凋亡的影响,揭示其抗损伤作用机制,为关节软骨损伤治疗提供理论依据。方法:分离培养人胚胎关节软骨细胞,采用四氮甲基唑蓝(MTT)法测定不同含量软骨细胞增殖活性的变化,利用光镜、电镜、DNA电泳及流式细胞仪测定作为凋亡检测指标。结果:IGF-Ⅰ呈剂量依赖式促软骨细胞增殖,当IGF-Ⅰ含量达50μg/L时,促软骨细胞增殖作用达最大值。IL-1组光镜、电镜下可见典型的细胞凋亡形态学改变,琼脂糖凝胶电泳示特征性的DNA梯状条带,IGF-Ⅰ处理组未见明显凋亡征象。流式细胞仪检测发现,IGF-Ⅰ处理后软骨细胞凋亡率显著降低。结论:IGF-Ⅰ能促进软骨细胞增殖,对IL-1诱导的软骨细胞凋亡具有保护作用。