An adenosine triphosphate bioluminescence assay for detecting the number of living cells]

The method for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r = 0.9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r = 0.9922). The detectable cells ranged from 10(2) to 10(6) cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility.     

弥漫性脾肿大脾血管的多普勒超声研究

多普勒超声是检查血流动力的一种理想方法 ,具有无创、安全、简便易行等优点。我们应用多普超声对各种常见弥漫性脾肿大患者脾动脉、脾静脉进行血流动力学测定 ,旨在发现其特征 ,为弥漫性脾肿大的病因鉴别诊断提供参考依据。1　资料与方法1.1　观察对象本研究检查弥漫性脾肿大患者 81例 ,男 55例 ,女 2 6例 ,年龄 16～ 6 5岁 ,均系我院住院患者 ,分为六组 ,血液病组 2 0例 ,肝硬化 2 0例 ,急性肝炎组 10例 ,慢性肝炎组 13例 ,伤寒组 7例 ,疟疾组 11例。对照组 32例 ,男 2 0例 ,女 12例 ,年龄 19～ 6 2岁 ,均系我院耳鼻喉科同期住院患者和来…     

Partial Sequence Analysis of the Genome of Human Herpesvirus 7 YY5 Isolated from Saliva Samples

Ｈｕｍａｎｈｅｒｐｅｓｖｉｒｕｓ 7(ＨＨＶ 7)ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｒｅｃｅｎｔｌｙｄｉｓｃｏｖｅｒｅｄｍｅｍｂｅｒｏｆｔｈｅｈｅｒ ｐｅｓｖｉｒｕｓｆａｍｉｌｙ .Ｉｔｗａｓｆｉｒｓｔｉｓｏｌａｔｅｄｆｒｏｍａｃｔｉｖａｔ ｅｄＣＤ4 Ｔｃｅｌｌｓｆｒｏｍｐｅｒｉｐｈｅｒａｌｂｌｏｏｄｍｏｎｏｎｕ ｃｌｅａｒｃｅｌｌｓ (ＰＢＭＣｓ)ｏｆａｈｅａｌｔｈｙｉｎｄｉｖｉｄｕａｌ[1] ,ａｐａｔｉｅｎｔｗｉｔｈｃｈｒｏｎｉｃｆａｔｉｇｕｅｓｙｎｄｒｏｍｅ(ＣＦＳ) [2 ] ,ａｎｄｆｒｏｍｓａｌｉｖａｏｆｈｅａｌ…     

乳癌保留乳房手术的探讨

①目的 探讨乳癌保留乳房手术的可行性。②方法 将９０例Ⅰ,Ⅱ期乳癌病人随机分为两组,保留乳房手术组４２例,采用乳腺区段切除加腋窝淋巴结清扫术;改良根治术组４８例,采用乳腺全切加腋窝淋巴结清扫术,术后两组均行放疗、化疗和内分泌治疗。比较两组治疗效果及并发症发生情况。③结果 乳癌保留乳房手术组３年生存率为９５．２％,５年生存率为９１．７％,发生并发症４例,改良根治术组３年生存率为９５．８％,５年生存率     

异丙酚氯胺酮混合液静脉麻醉

选择 40例 ASA ～ 级病人行异丙酚氯胺酮 (1∶ 2 )复合静脉麻醉。结果麻醉诱导后血压、心率与麻醉前相比无明显变化 ,2 0 %病人出现一过性 SPO2 下降 (<94% )。麻醉诱导苏醒快 ,满意率高 ,恢复期无精神症状。表明异丙酚可控制氯胺酮的心血管兴奋作用和恢复期精神症状 ,氯胺酮可减轻异丙酚的心血管抑制作用。两者配伍是一种较好的短效静脉麻醉方式 ,但仍存在呼吸抑制作用 ,应引起注意。     

职业人群不健康行为生活方式与高尿酸血症的关系及高血压、血脂异常的效应修饰作用

目的 探索不健康行为生活方式与高尿酸血症的关系,以及高血压、血脂异常的效应修饰作用,为预防高尿酸血症提供理论依据。方法 采用横断面调查研究设计,基于2021年10-12月来自四川省、贵州省28个地级市和重庆市33个区（县）中国铁路成都局集团有限公司的西南职业人群队列基线数据,通过问卷调查、体格测量及实验室生化检测收集研究对象的人口学特征、行为生活方式、慢性非传染性疾病患病情况。不健康行为生活方式得分根据吸烟、饮酒、膳食模式、体力活动和低体重/超重状况进行评分,分值越高不健康行为生活方式越多。采用多因素logistic回归模型分析不健康行为生活方式评分、吸烟状况、饮酒状况等与高尿酸血症的关系,采用分层分析探索高血压等疾病对不健康行为生活方式与高尿酸血症之间关系的修饰效应。结果 共纳入11 748名研究对象,高尿酸血症患病率为34.4%。多因素logistic回归分析显示,现在吸/既往吸烟、现在饮/既往饮酒及BMI异常是高尿酸血症患病的危险因素,不健康行为生活方式对高尿酸血症患病风险呈现累积效应,随着得分的升高,高尿酸血症患病风险升高,OR值由1.64（95%CI：1.34~2.00）上升至2.89（95%CI：2.39~3.50）。分层分析结果显示,在高血压及血脂异常人群中,不健康行为生活方式对高尿酸血症患病风险影响更大。结论 多种不健康行为生活方式的共存会升高高尿酸血症患病风险,这一效应在高血压、血脂异常人群中更明显。及时纠正不健康行为生活方式,并控制高血压和血脂异常,降低患高尿酸血症的风险。     

大鼠肝细胞线粒体三维结构的观察与重建

为深入研究大鼠肝细胞线粒体形态特征,本研究以钻石刀连续超薄切片,通过电子显微观察大鼠肝细胞线粒体的三维结构,对特殊形态的线粒体进行三维结构重组。结果表明：球形,椭球形,盘状及杆状等小型线粒体约占线粒体总数的５３．８％,而分枝形及各种不规则形的线粒体约占４１．６％。     

A novel cytoplasmic protein with RNA-binding motifs is an autoantigen in human hepatocellular carcinoma

In hepatocellular carcinoma (HCC), autoantibodies to intracellular antigens are detected in 30-40% of patients. Patients with chronic hepatitis or liver cirrhosis develop HCC, and when this occurs, some patients exhibit autoantibodies of new specificities. It has been suggested that these novel autoantibody responses may be immune system reactions to proteins involved in transformation-associated cellular events. One HCC serum shown to contain antibodies to unidentified cellular antigens was used to immunoscreen a cDNA expression library, and a full length cDNA clone was isolated with an open reading frame encoding 556 amino acids with a predicted molecular mass of 62 kD. The 62-kD protein contained two types of RNA-binding motifs, the consensus sequence RNA-binding domain (CS-RBD) and four hnRNP K homology (KH) domains. This protein, provisionally called p62, has close identity (66-70%) to three other proteins at the amino acid sequence level, and all four proteins may belong to a family having CS-RBD in the NH2-terminal region and four KH domains in the mid-to-COOH- terminal region. The homologous proteins are: KH domain-containing protein overexpressed in cancer (Koc); zipcode binding protein, a protein which binds to a conserved nucleotide element in chicken beta-actin mRNA (ZBP1); and a protein which binds to a promoter cis element in Xenopus laevis TFIIIA gene (B3). p62 protein is cytoplasmic in location, and autoantibodies were found in 21% of a cohort of HCC patients. Patients with chronic hepatitis and liver cirrhosis, conditions which are frequent precursors to HCC, were negative for these autoantibodies, suggesting that the immune response might be related to cellular events leading to transformation. However, the possible involvement of p62 autoantigen as a factor in the transformation process remains to be elucidated.     

住院病人焦虑的原因及护理对策

目的：分析住院病人焦虑的原因,有效地减轻住院病人的焦虑程度。方法：使用焦虑自评量表（SAS）及问卷对347例住院病人进行焦虑原因的调查分析。结果：问卷20题中有13项肯定组与否定组的SAS评分存在显著差异或非常显著差异;不同年龄、不同文化程度、不同住院时间、治疗费用不同支出方式的住院病人SAS评分也存在显著差异。结论：明确住院病人焦虑原因,可有针对性地对其做好心理护理。