Induction of the liver cancer-down-regulated long noncoding RNA uc002mbe.2 mediates trichostatin-induced apoptosis of liver cancer cells

Differential expression of long non-coding RNAs (lncRNAs) plays critical roles in hepatocarcinogenesis. Considerable attention has focused on the antitumor effect of histone deacetylase inhibitor (Trichostatin A, TSA) as well as the coding gene expression-induced apoptosis of cancer cells. However, it is not known whether lncRNA has a role in TSA-induced apoptosis of human hepatocellular carcinoma (HCC) cells. The global expression of lncRNAs and coding genes was analyzed with the Human LncRNA Array V2.0 after 24 h treatment. Expression was verified in cell lines and tissues by quantitative real-time PCR. The data showed that 4.8% (959) of lncRNA and 6.1% (1849) of protein coding gene were significantly differentially expressed. The differential expressions of lncRNA and protein coding genes had distinguishable hierarchical clustering expression profiling pattern. Among these differentially expressed lncRNAs, the greatest change was noted for uc002mbe.2, which had more than 300 folds induction upon TSA treatment. TSA selectively induced uc002mbe.2 in four studied HCC cell lines. Compared with normal human hepatocytes and adjacent noncancerous tissues, uc002mbe.2 expression level was significantly lower in the HCC cell lines and liver cancer tissues. The TSA-induced uc002mbe.2 expression was positively correlated with the apoptotic effect of TSA in HCC cells. In addition, knockdown the expression of uc002mbe.2 significantly reduced TSA-induced apoptosis of Huh7cells. Therefore, TSA-induced apoptosis of HCC cells is uc002mbe.2 dependent and reduced expression of uc002mbe.2 may be associated with liver carcinogenesis.     

多发性骨髓瘤患者骨髓骨硬化蛋白表达及其临床意义分析

目的探讨多发性骨髓瘤(MM)患者骨髓上清液骨硬化蛋白(sclerostin)的表达水平及其临床意义。方法采用酶联免疫吸附试验法定量检测sclerostin的水平,逆转录-聚合酶链反应(RT-PCR)检测骨髓单核细胞(BMMNC)sclerostin基因的表达。结果 MM组sclerostin水平为(0.54±0.21)pg/mL,明显高于对照组(0.31±0.06)pg/mL,差异有统计学意义(t=5.67,P<0.01);复发难治组sclerostin水平为(0.65±0.17)pg/mL,明显高于对照组及初诊组(0.47±0.21)pg/mL,差异有统计学意义(t=8.44、3.27,P<0.01),RT-PCR也证实了部分MM患者的BMMNC能表达sclerostin基因;MM组sclerostin水平与碱性磷酸酶(ALP)呈负相关(r=-0.379,P=0.005),与校正血钙、骨质损害评分、血清β2-微球蛋白、M蛋白的比例、临床ISS分期呈正相关;中位随访时间29(6~65)个月,低sclerostin组中位生存期为48(6~65)个月,高sclerostin组中位生存期为24(6~52)个月,二者比较差异有统计学意义(χ2=12.74,P<0.01)。结论骨髓sclerostin水平可反映MM患者骨质破坏、成骨受抑程度及机体瘤负荷水平,并能在一定程度反映患者的中位生存期。     

应加强医学院本科肿瘤学教育

通过对本校医学院20年本科肿瘤学教育的经验总结,阐明在医学院校中开展肿瘤学教育的必要性?肿瘤学并不是小科目;在教学中应重视对基础知识的复习,突出专业知识,解决难点?疑点;灌输循证医学的理念,同时内容要新颖?时效性要强?肿瘤学课程决不是简单的重复,它把多学科的理论知识融合在一起,灌输给学生的是综合分析的能力?因此必须加强医学院校本科肿瘤学的教育,为培养合格临床肿瘤科医生而努力?     

巴蜀中医药古籍保护与开发的现状及思考

古籍数字化建设是当今图书馆的一大趋势,也是当今图书馆发展方向。本文介绍了巴蜀中医药古籍保护与开发现状、存在的问题,提出了三种巴蜀中医药古籍保护与开发的意义及对策。     

非糖尿病孕妇分娩巨大儿的相关因素及围产儿结局

目的探讨非妊娠糖尿病孕妇发生巨大儿的相关因素及围产儿的结局情况。方法选择我院进行非妊娠期糖尿病孕妇分娩的巨大儿资料进行回顾性的分析,与同期分娩的无合并症产妇正常体重儿资料进行对比。结果孕妇宫高、腹围、胎儿双顶径≥9．6cm、股骨长≥7．6em、腹围≥37cm、肥胖、体重增加≥20奴,两组比较差异有统计学意义（P〈0．05）。观察组剖宫产、产后出血、发生软产道裂伤、新生儿产伤、新生儿窒息较对照组增多,差异有统计学意义（P〈0．05）。结论加强非糖尿病孕妇围生期的保健管理工作,产前正确地预测巨大儿并选择合适的分娩方式,对降低剖宫产率及减少母婴并发症具有重大意义。     

No overt structural or functional changes associated with PEG-coated gold nanoparticles accumulation with acute exposure in the mouse heart

In this study, we investigated the cardiac biodistribution of polyethylene glycol (PEG)-coated AuNPs and their effects on cardiac function, structure and inflammation in both normal and cardiac remodeling mice. The model of cardiac remodeling was induced by subcutaneously injection of isoproterenol (ISO), a non-selective beta-adrenergic agonist, for 7 days. After AuNPs were injected intravenously in mice for 7 consecutive days, Au content in different organs was determined quantitatively by inductively coupled plasma mass spectrometry (ICP-MS), cardiac function and structure were measured by echocardiography, cardiac fibrosis was examined with picrosirius red staining, the morphology of cardiomyocytes was observed with hematoxylin and eosin (H & E) staining. The accumulation of AuNPs in hearts did not affect cardiac function or induce cardiac hypertrophy, cardiac fibrosis and cardiac inflammation under normal physiological condition. Cardiac AuNPs content was 6-fold higher in the cardiac remodeling mouse than normal mice. However, the increased accumulation of AuNPs in the heart did not aggravate ISO-induced cardiac hypertrophy, cardiac fibrosis or cardiac inflammation. These observations suggest that PEG-coated AuNPs possess excellent biocompatibility under both physiological and pathological conditions. Thus, AuNPs may be safe for cardiac patients and hold great promise for further development for various biomedical applications.     

首批N（2）-L-丙氨酰-L-谷氨酰胺国家对照品的研制

目的建立首批N（2）-L-丙氨酰-L-谷氨酰胺国家对照品。方法应用红外光谱、液质联用仪等对N（2）-L-丙氨酰-L-谷氨酰胺进行结构确证,采用HPLC法测定纯度及氨基酸比值,采用3种方法进行含量测定。结果确定首批N（2）-L-丙氨酰-L-谷氨酰胺对照品的含量为99.8%。结论首批N（2）-L-丙氨酰-L-谷氨酰胺国家对照品可用于N（2）-L-丙氨酰-L-谷氨酰铵及其制剂的定性和定量控制。     

MicroRNA-10 negatively regulates inflammation in diabetic kidney via targeting activation of the NLRP3 inflammasome

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Antiproliferative Activity of the Chinese Medicinal Compound,Delisheng, Compared With Rg3 and Gemcitabine in HepG2 Cells

Delisheng consists of radix ginseng, radix astragali, venenum bufonis and mylabris. It has been reported that delisheng inhibits the proliferation of adenocarcinoma cells and stimulates their apoptosis. Delisheng can also enhance the body''s immunity and induce the redifferentiation of carcinoma cells. Delisheng inhibited the proliferation of HepG2 cells in MTT assay and promoted apoptosis more effectively in contrast to the active components of ginseng extract, Rg3 and gemcitabine. It is possible that Rg3 has an important role in delisheng because they all could regulate the cell cycle, apoptosis and expression of endostatin and VEGFR-2. Delisheng caused the cell cycle to arrest at the S phase, while gemcitabine blocked the cells at the G0/G1 phase in cell cycle analysis. Consequently, the apoptosis rate of the HepG2 cell line can be increased significantly by delisheng in combination with gemcitabine, compared with the single drug. The expression of the procaspase proteins, caspase protein, and dr5 detected by Western blot were increased while bcl-2 and survivin decreased in the delisheng group, compared with controls. The observations suggest that the delisheng induced apoptotic effect might be closely related to the mitochondrial apoptosis pathway, and the death receptor signaling pathway.     

综合气道管理对卒中相关性肺炎患者平均住院时间及预后的影响

目的探讨综合呼吸道管理对卒中相关性肺炎患者平均住院时间及预后的影响。方法采集自2010年4月至2011年6月发生卒中相关性肺炎患者90例,其中采用综合呼吸道管理45例为干预组,因患方原因而呼吸道管理不完善45例为对照组,分别统计住院时间、预后、抗生素使用时间等指标。结果干预组平均住院时间10.32 d,其中家属签字出院2例,肺炎治愈好转出院43例;对照组平均住院时间11.52 d,其中家属签字出院16例,肺炎治愈病情好转出院29例,两组住院时间比较,差异无统计学意义(P=0.085)。两组好转率比较,差异有统计学意义(P=0.007)。干预组平均使用抗生素9.50 d,对照组使用11.01 d,两组比较,差异有统计学意义(P=0.042);神经功能美国国立卫生研究院卒中量表(NIHSS)评分比较出院、入院评分差值,干预组为4.3分,对照组为2.1分,两组比较,差异有统计学意义(P=0.021)。结论进行综合呼吸道管理可以改善卒中相关性肺炎患者预后,减少抗生素使用,有利于卒中康复。