化瘀通络中药对肾衰大鼠肾组织CTGF蛋白表达的实验研究

目的：采用蛋白质印迹法（Western Blot）检测化瘀通络中药肾络通对5／6肾切除肾衰大鼠残余肾结缔组织生长因子（CTGF）蛋白表达的影响。方法：5／6肾切除制备肾衰大鼠模型，随机分为假手术组、模型组、肾络通组和缬沙坦组，分别灌胃给药16周取材，应用Western Blot法检测肾组织CTGF蛋白的表达。结果：模型组与假手术相比，CTGF蛋白的表达明显增高；肾络通组CTGF蛋白表达较模型组显著降低。结论：肾络通可使慢性进展性纤维化肾组织CTGF蛋白表达下调，延缓5／6肾切除肾衰大鼠的病程进展，提示以CTGF为切入点，运用不同的有效中药组方对其加以研究，将为肾纤维化防治提供新的思路。     

测定加替沙星眼凝胶中加替沙星的含量

目的建立高效液相色谱法测定加替沙星眼凝胶中加替沙星含量。方法用Nucleosil 120-5 C18 (4.0 mm×250 mm,5 μm),以磷酸二氢钾缓冲液(磷酸二氢钾1 g溶解于纯化水1 000 mL,用磷酸调节pH值4.0) 甲醇(70∶30)为流动相,检测波长：286 nm,流速：1.0 mL·min^-1,柱温：30℃。结果加替沙星在10～50 μg·mL^-1浓度范围内呈良好的线性关系,平均回收率99.3%,RSD＝0.63%。结论该方法简便、快速、专属性强,可用于加替沙星眼凝胶中加替沙星的含量测定。     

大黄素和器官纤维化

探讨大黄素抗器官纤维化的作用机制,阐明大黄素可作为治疗器官纤维化的有效中药制剂.     

健康成年人高通噪声掩蔽听性脑干反应研究

目的 研究健康成年人高通噪声掩蔽听性脑干反应(又称为耳蜗积水掩蔽分析程序,the cochlear hydrops analysis masking procedure,CHAMP)的特点,探讨CHAMP各参数在诊断膜迷路积水中的应用价值.方法 应用Bio-logic听性诱发电位系统对20名(40耳,其中男女各10名)耳科正常人记录由6种声信号所诱发的听性脑干反应(auditory brainstem response,ABR),包括:单独使用短声及截断点分别为8、4、2、1、0.5 kHz同侧高通滤过粉红噪声(high-pass masking pink noise)掩蔽下的短声信号.结果 高通滤过粉红噪声掩蔽下的短声所诱发的ABR V波潜伏期较短声ABR长,短声+8、4、2、1、0.5 kHz高通滤过粉红噪声的V波潜伏期延迟平均((x)±s,下同)分别为(0.30±0.18)、(0.97 ±0.43)、(1.65±0.64)、(3.21±0.56)、(4.66±0.37)ms.短声+0.5 kHz高通滤过粉红噪声诱发的ABR与短声ABR的复合振幅比为0.95 ±0.11.结论 对于膜迷路积水,CHAMP可能是一种很有前景的诊断方法,V波潜伏期延迟可作为判定CHAMP正常与否的标准,但复合振幅比的特异性有待进一步验证.

Abstract：

Objective To study the characteristic of the cochlear hydrops analysis masking procedure ( CHAMP) in normal adults, and to evaluate the diagnostic values of its parameters for logic auditory evoked potential system: clicks presented alone (unmasked condition) and clicks presented with ipsilateral pink noise high-pass filtered at 8 , 4, 2, 1, and 0. 5 kHz respectively. Results The wave V latency of ABR to the high-pass masking pink noise clicks were longer than ABR to clicks alone. The latency delays of wave V for clicks presented with ipsilateral pink noise high-pass filtered at 8 , 4, 2, 1, and 0.5 kHz compared to clicks alone were (0.30 ±0.18), (0.97 ±0.43), (1.65 ±0.64), (3.21 ±0.56),(4. 66 ± 0. 37 ) ms respectively. The complex amplitude ratio between ABR to click + 0. 5 kHz high-pass noise and click alone was 0.95 ±0.11. Conclusions CHAMP is a promising diagnostic method for membranous labyrinth hydrops, and the latency delay of wave V might be used as the normal criterion. The specificity of the complex amplitude ratio need furthur evaluation in clinical work.     

大剂量α-硫辛酸治疗糖尿病周围神经病变的有效性和安全性评价

目的 观察口服较大剂量α-硫辛酸对糖尿病神经病变的治疗作用与安全性.方法 采用随机、双盲、多中心与安慰剂对照方法,共从5个中心入选236例有临床症状的糖尿病神经病变患者,口服α-硫辛酸(117例)1800 mg/d或安慰剂(119例)12周,主要终点指标为总神经症状积分,次要终点指标包括神经传导速度、各神经症状积分、HbA1c和安全性指标,分别在随访前和治疗后2、4、8、12周观察这些指标的变化,统计分析两组间差别.结果 经过α-硫辛酸治疗12周后,73.3%患者神经症状显著改善,对照组为18.3%;总神经症状积分减少2.6±2.3,显著高于对照组的0.7±1.4(P＜0.05);2周随访时,两组症状改变量差异有统计学意义(P＜0.05).α-硫辛酸对疼痛、麻木、烧灼感和其他感觉异常均有较好的疗效,症状积分均较入组前显著减少(P＜0.05);但是神经传导速度在治疗前后无改变.12周观察期间,α-硫辛酸组HbA1c进一步降低;但是副反应发生率较高(25.4%),以咽喉部至胃部灼热感为主,未见严重不良反应.结论 在安全的基础上,口服较大剂量α-硫辛酸对改善糖尿病神经病变的症状疗效显著,在2周内就能使症状显著减轻.     

Inhibition of tumor metastasis in vivo by combination of paclitaxel and hyaluronic acid

There is little information about the inhibitory effects of hyaluronic acid with paclitaxel on tumor metastasis and ascites formation. The aim of present study is to determine whether the combined administration of hyaluronic acid (HA) with paclitaxel can produce additional or synergistic therapeutic effects in the control of Lewis lung carcinoma (LLC) migration and ascites formation of U14 cervical tumor. The anti-metastasis effect of hyaluronic acid alone, paclitaxel alone and paclitaxel-hyaluronic acid combination were examined in a mouse model bearing LLC cells. i.v. Paclitaxel-hyaluronic acid, in the ratio of 1:3 (w/w), significantly reduced the migration of LLC cells in a synergistic fashion. In the experiment with mice bearing U14 tumor cells, i.p. paclitaxel-hyaluronic acid markedly improved the life span of mice and significantly decreased ascites formation when compared to paclitaxel alone or hyalunonan alone. We also analyzed the serum proteome of mice with lung neoplasm before and after treatment with paclitaxel-hyaluronic acid. Proteomic analysis on LLC mice was studied using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry. The results showed that i.v. administration of hyaluronic acid in combination with paclitaxel had significantly up-regulated the expression of vitamin D(3) binding proteins (DBP), which is a macrophage-stimulating activator. In conclusion, the in vivo anti-metastasis effect was associated with the synergistic therapeutic effects of hyaluronic acid-paclitaxel combination and a significant promotion of immune proteins expression. These results demonstrated that the combination of paclitaxel and hyaluronic acid may be an effective way to inhibit tumor migration.     

中西医结合传染病研究的目标与任务

中西医结合传染病研究应该从临床和基础两个层次,在病毒性肝炎、艾滋病、结核病和新发传染病等重点领域,开展感染性疾病的免疫调节,细菌、病毒耐药株的治疗,以及感染性疾病的功能和组织学恢复等研究,瞄准现代医学的薄弱环节和不足之处,发掘中医学的宝贵经验和理论优势,进行临床验证和机理研究．以纳入现代医学的框架,为世界医学做出贡献。     

错畸形牙弓形态的模拟及比较

目的 :比较不同类型错牙合畸形的牙弓形态。方法 :采用三维测量仪精密测量正常牙合及 4组不同类型错牙合(ClassⅠ双颌前突、ClassⅡ1、ClassⅡ2 、ClassⅢ )的原始模型 ,圆锥曲线模拟其牙弓形态。结果 :不同类型错牙合牙弓形态的差异有显著性 ,甚至某些分组的上下颌之间牙弓形态的差异也有显著性。结论 :临床上弯制弓丝时采用单一的标准牙弓形态是不完全科学的 ,应考虑错牙合的不同类型及其矫治前不同的牙弓形态以防止畸形复发。