乳兔雪旺细胞对成兔视神经挫伤修复的作用

目的 研究乳兔雪旺细胞(Schwann cell,SC)对成兔视神经挫伤修复的作用。 方法 建立成兔视神经挫伤模型,伤后24 h分别向伤眼玻璃体腔内注入SC悬液(A组)、生理盐水(B组)各0.1 ml。伤后不同时间点进行视网膜节细胞(retinal ganglion cell,RGC)、轴突染色记数及闪光视觉诱发电位(flash visual evoked potentials,FVEP)检测。 结果 伤后4周A、B组RGC平均记数分别为(19.89±3.79) /mm和(12.67±4.12) /mm,轴突密度分别为(94.569±793) /mm ²和(36.085±285) /mm²,A组明显高于B组(P<0.01)。伤后3 d A组伤眼与健眼FVEP幅值比由48%上升至88%,8周时仍为78%,各时间点均明显高于B组(P＜0.01)。 结论 乳兔SC能够提高成兔视神经挫伤后RGC存活率,减轻轴突变性,显著促进视神经功能恢复,对视神经挫伤修复具有明显的促进作用。(中华眼底病杂志,2000,16:91-93)      

Inhibitory effects of licochalcone A isolated from Glycyrrhiza inflata root on inflammatory ear edema and tumour promotion in mice

Licochalcone A, 3-a,a-dimethylallyl-4,4'-dihydroxy-6-methoxychalcone, from the root of Glycyrrhiza inflata Beta (Leguminosae) (Xin-jiang liquorice) showed anti-inflammatory action towards mouse ear edema induced by arachidonic acid (AA) and 12-O-tetradecanoylphorbol 13-acetate (TPA) by topical application. Anti-tumour promoting action of licochalcone A was also observed in vivo for mouse skin papilloma initiated by dimethylbenz[a]anthracene (DMBA) and promoted by TPA. It inhibited in vitro 32Pi-incorporation to phospholipids in HeLa cells promoted by TPA. A competitive interaction of licochalcone A with the TPA-receptors in the cell membrane has been suggested.     

Identification of a major metabolite of danshensu in rat urine and simultaneous determination of danshensu and its metabolite in plasma: application to a pharmacokinetic study in rats

Danshensu, as the effective component of Salvia miltiorrhiza (Danshen), has been widely used in clinical studies for treatment of cardiovascular diseases in China. A new metabolite, 4‐hydroxy‐3‐methoxyphenyllactic acid was isolated from the urine of rats, and its chemical structure was identified by ultraviolet (UV), Infrared Spectroscopy (IR), mass spectrometry (MS) and nuclear magnetic resonance (NMR). Furthermore, a selective and sensitive high performance liquid chromatography‐tandem mass spectrometric (HPLC‐MS/MS) method was developed for the simultaneous quantification of danshensu and its major metabolite, 4‐hydroxy‐3‐methoxyphenyllactic acid, in rat plasma after oral and intravenous administration of danshensu. The separation was performed on a Hypersil Gold C₁₈ column (150 × 2.1 mm i.d., 3.0 µm, Thermo, San jose CA, USA) with gradient elution using a mobile phase composed of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. Linear detection responses were obtained for danshensu and 4‐hydroxy‐3‐methoxyphenyllactic acid ranging from 5 to 10000 ng/mL and 5 to 4000 ng/mL, respectively. The lower limits of quantification (LLOQs) for the two compounds were both 5 ng/mL. The intra‐and inter‐day precision (R.S.D %) were within 5.61% for the two analytes. The average recoveries of the analytes were greater than 72.43%. The method was proved to be stable during all sample storage, preparation and analytic procedures. This method was successfully applied to the pharmacokinetic studies of danshensu and 4‐hydroxy‐3‐methoxyphenyllactic acid after oral and intravenous administration of danshensu in rats.Copyright © 2014 John Wiley & Sons, Ltd.     

金钱草中黄酮苷的分离与结构鉴定金钱草中黄酮苷的分离与结构鉴定

目的从金钱草中分离制备标准品。方法用硅胶柱色谱进行分离,通过理化方法及IR,UV,MS,¹HNMR,¹³CNMR,DEPT,HMQC,HMBC等光谱分析确定化合物结构。结果从金钱草中分离得到2个化合物,分别鉴定为鼠李酮酸γ-内酯(I)、山奈酚-3-O-α-L-鼠李糖(1→2)-β-D-木糖苷(II)。结论II为新化合物,命名为金钱草素,I为首次从该属植物中分离得到。     

人参多糖对肾病患者外周血单核细胞诱生白细胞介素－2及其受体活性影响

通过体外研究证实，人参多糖对健康人和乙肝相关肾炎、肾病综合征和慢性肾功能哀竭患者外周血单核细胞诱生白细胞介素－２及其受体活性具有显著的促进作用，且在所试验的剂量范围内对健康人和三种肾病患者的作用均呈量效依赖性。毒性试验表明人参多糖对血细胞无毒性作用。认为人参作为一种有效生物反应调节剂，应用于临床具有重要的实用和开发价值。     

白族药八边叶（八角枫）的研究进展

八边叶,白族民间用于治疗风湿性关节炎、跌打损伤、腰肌劳损等。其优点是疗效可靠,药源广泛。通过查阅关于八边叶研究文献,对八边叶主要化学成分、药理作用及民间用药经验进行了总结,以期为八边叶的深入研究和开发利用提供科学依据。     

高寒藏药──川西獐牙菜组织培养研究Ⅰ.愈伤组织的诱导及初步培养

从川西獐牙菜的胚轴、未成熟种子及幼叶诱导出愈伤组织；试验选用MS、B5和N63种培养基，其中以加有2，4－D3mg／L＋KT0.6mg／L的MS培养基诱导率最高。结果表明,不同培养基及不同激素组合对愈伤组织诱导率有较明显的影响。初步证明了用植物组织培养的方法进行川西獐牙菜的无性快速繁殖是有可能的。     

逍遥散对肝郁脾虚证模型大鼠海马TPH2与IDO1的调节作用

目的:观察肝郁脾虚证模型大鼠海马色氨酸羟化酶2(TPH2)与吲哚胺-2,3-双加氧酶1(IDO1)表达的变化并探讨逍遥散的调节作用。方法:雄性SD大鼠随机分成4组,分别是正常组、模型组、逍遥散组、氟西汀组,每组6只。采用慢性束缚应激的方法制备大鼠肝郁脾虚证模型,造模持续21 d。通过荧光定量PCR与Western-blot方法检测各组大鼠海马TPH2与IDO1的表达情况。结果:模型组大鼠海马TPH2 mRNA的表达水平低于其余3组大鼠(P0.05),逍遥散与氟西汀对TPH2 mRNA的表达有一定的上调作用;模型组大鼠海马IDO1 mRNA的表达显著增加(P0.01),逍遥散与氟西汀对IDO1 mRNA表达的下调作用明显(P0.01),且逍遥散对IDO1 mRNA的下调作用更明显。模型组、逍遥散组、氟西汀组大鼠海马TPH2的蛋白的表达低于正常组(P0.01),逍遥散组TPH2蛋白表达高于模型组(P0.05);模型组大鼠海马IDO1的蛋白表达显著高于其余3组(P0.01)。结论:肝郁脾虚证模型大鼠海马TPH2的表达减少,IDO1的表达增多,逍遥散可能通过调节海马TPH2与IDO1的表达水平进而影响5-HT的含量,起到治疗作用。     

中药异位宁对实验性大鼠异位子宫内膜COX-2基因表达的影响

目的：探讨环氧合酶-2（COX-2）在子宫内膜异位症（EMT）发病中的作用以及异位宁对其基因表达的影响。方法：采用自体子宫组织块移植法建立子宫内膜异位症大鼠模型。应用免疫组化及原位杂交的方法,检测异位子宫内膜中COX-2的表达。结果：模型组EMT大鼠异位内膜中COX-2基因表达增高明显,与正常对照组比较具有显著性差异（P〈0.01）;中药高剂量组COX-2基因表达明显减弱,与模型组比较,有显著性差异（P〈0.05、P〈0.01）。结论：中药异位宁具有抑制COX-2阳性表达,从而抑制异位内膜组织新生血管形成,达到治疗EMT的作用。