Transforming Growth Factor-β2 mRNA Level in Unloaded Bone Analyzed by Quantitative In Situ Hybridization

The effects of tail suspension hypokinesia on the gene expression for TGF-β₂ at different sites within bone were evaluated. TGF-β₂ mRNA signal levels were determined quantitatively by an image analysis system. The osteopenia induced by tail suspension was verified by histomorphometry. In the periosteum of nonsuspended control rats, TGF-β₂ mRNA was highly expressed in the preosteoblasts and osteoblast-rich cambial layers; very little signal was present within the middle and outer fibroblastic layers. Gene expression was significantly reduced in suspended rats, and this was evident both in terms of the number of silver grains in unit area or length of tissue and in each osteoblast and preosteoblast. Hypokinesia also reduced the expression of TGF-β₂ mRNA level in cortical and trabecular bone osteocytes, but did not adversely affect the mRNA level in chondrocytes in growth plate. The results affirm the site-specific response of TGF-β₂ gene expression in rats, and suggest that the cortical and trabecular bone osteopenia associated with hypokinesia in rats may be associated with a deficit in osteoblastic and osteocytic TGF-β₂ level. Received: 6 February 1998 / Accepted: 10 November 1998     

Simultaneous irradiation and imaging of blood vessels during pulsed laser delivery

BACKGROUND AND OBJECTIVE: Simultaneous irradiation and viewing of 10-120 microm cutaneous blood vessels were performed to investigate the effects of 2-micros 577-nm dye laser pulses. STUDY DESIGN/MATERIALS AND METHODS: A modified scanning laser confocal microscope recorded vessel response to different radiant exposures (J/cm2). Probit analysis determined the 50% probability ("threshold") radiant exposure necessary to cause embolized or partly occluding coagula, coagula causing complete blood flow stoppage, and hemorrhage. RESULTS: A statistically significant difference in the threshold radiant exposure existed for each damage category for blood vessels 10-30 microm in diameter, but not for larger vessels. For vessels over 60 microm, complete flow stoppage was unattainable; increasing laser pulse energy produced hemorrhage. In larger vessels, coagula often were attached to the superficial vessel wall while blood flowed underneath. Monte Carlo optical and finite difference thermal modeling confirmed experimental results. CONCLUSION: These results provide insight into the role of pulse duration and vessel diameter in the outcome of pulsed dye laser irradiation.     

The experience in the treatment of patients with extensive full-thickness burns

The aim of the present study is to analyze the changes of cure rate and the main causes of death in the last 40 years, and to summarize our experience in the treatment of extensive full-thickness burn patients. The clinical characteristics, cure rate and main causes of death of 73 cases with total burned area more than 90% TBSA and full-thickness burn area more than 70% TBSA were analyzed retrospectively. Among them, 21 cases (28.8%) were cured and 52 cases (71.2%) died. The cure rate increased significantly in the recent years, and the main causes of death changed from shock and sepsis in the time period 1959–1978 to sepsis and MODS in the past two decades. Due to the improvement of early comprehensive management of burn shock, aggressive surgical approach to full-thickness burn wound and potent systemic supporting measures, the survival rate increased significantly.     

脂肪酸、二磷酸果糖和谷氨酰胺对大鼠移植小肠粘膜细胞增殖和凋亡的作用

目的探讨ｎ３脂肪酸、１,６二磷酸果糖和谷氨酰胺对移植小肠粘膜细胞增殖和凋亡的作用。方法１９６只近交系Ｗｉｓｔａｒ大鼠分别作为供、受体行全小肠异位移植,术前和术后分别用ｎ３脂肪酸灌胃、１,６二磷酸果糖及谷氨酰胺静脉输注１０天,应用流式细胞术和细胞凋亡原位检测的方法分析小肠粘膜细胞增殖和凋亡的变化。结果移植小肠粘膜细胞增殖低下,凋亡增加。补充外源性ｎ３脂肪酸、１,６二磷酸果糖和谷氨酰胺后,移植小肠粘膜细胞的增殖加速,凋亡减少。结论ｎ３脂肪酸、１,６二磷酸果糖和谷氨酰胺特殊营养支持可显著地增加移植小肠粘膜细胞的增殖活性,同时也不同程度地抑制细胞凋亡发生,这种调控作用有助于改善移植小肠的结构和吸收功能。     

内毒素休克大鼠组织生物喋呤及其合成限速酶基因表达的改变

目的 了解内毒素休克时重要器官生物喋呤及其合成限速酶基因表达的改变和病理生理意义。方法 采用大鼠内毒素休克模型,检测肝,肾,肠等组织生物喋呤含量,三磷酸鸟苷环水解酶Ｉ（ＧＴＰ－ＣＨＩ）ｍＲＮＡ表达及器官功能指标等。并观察内毒素拮抗剂－重组杀菌／通透性增加蛋白（ｒＢＰＩ２１）的防治效果。结果 内毒素休克动物肝,肾,肠等组织生物喋呤含量明显增多,伤后８小时升高幅度尤为显著（Ｐ〈０．０５,Ｐ〈０．０１０     

精氨酸对烧伤创面愈合的影响

目的 探讨精氨酸对烧伤创面愈合的影响和量效关系。方法 通过大鼠深Ⅱ度烫伤模型,伤后肠道站充不同剂量精氨酸,观察烧伤创面愈合时间,测定烧伤创面愈合面积百分比,创面Ｉ型／Ⅲ型胶原比例,羟脯氨酸（ＯＨＰ）含量和真皮细胞增殖周期,结果 每天补充１００－４００ｍｇ／ｋｇ精氨酸可缩短烧伤创面愈合时间,增加烧伤创面中ＯＨＰ含量,降低烧伤创面Ｉ／Ⅲ型胶原比例,加速真皮细胞ＤＮＡ复制。比较喂不同剂量精氨酸各组,发现     

胃肠道癌手术与门静脉单细胞转移的相关性研究

目的 进一步了解在胃癌、大肠癌的根治过程中,手术对单细胞转移的影响。方法 １７２例２分２组,阳性受检组１１１例,先结扎肿瘤区引渡以血和的阴性对照组６１例。开腹探后将导管置入门静脉抽取血标本,抗上皮细胞膜克隆抗体为探针,ＡＢＣ免疫组化法检测门静脉癌细胞。结果 阳性受检组１１１例患者中有７２例发现肿瘤细胞、阳性率为６４．９％,有性对照组的６１例仅发现１３例,阳性率为２１．３％,两组且间差异有显著性,     

窥镜下激光治疗前列腺增生症

目的 评价经尿道激光前列腺切除术（ＴＵＬＰ）的疗效及其与前列腺大小的关系。方法 回顾分析了１９８８年至１９９８年１０年,采用ＴＵＬＰ治疗的４６９例前列腺增生症患者对手术技术、手术前后的症关改善情况进行比较,并对２３３例符合条件的患者ＴＵＬＰ手术效果与前列腺大小的相关性进行了研究。结果 ４６９例患者前列腺症状评分（ＩＰＳＳ）平均值由术前的２６．７降到术后的１４．２;最大尿流率平均值由６．２ｍｌ／ｓ上     

甲状腺癌的颈淋巴结转移与血行转移

目的 探讨甲状腺颈淋巴结转移及血行转移的有关因素,提高甲状腺癌的诊断及治疗水平。方法 对１９８４年１月￣１９９２年１２月收治的２２５例甲状腺癌患者的临床特点、治疗方法及病理结果进行了回顾性分析总结。结果 甲状腺癌的淋巴结及血行转移主要现理类型和肿瘤局部浸润程度有关。乳头状癌、鳞状细胞癌主要表现为区域淋巴结转移,转移率分别为４４．７％、３／３,晚期出现血行转移。滤泡状腺癌虽然分化较好,但多表现有血行