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61.
Zeinab ASKARI Hamed MIRJALALI Mehdi MOHEBALI Zabih ZAREI Saeideh SHOJAEI Tahereh REZAEIAN Mostafa REZAEIAN 《Iranian Journal of Parasitology》2015,10(3):381-388
Background: Microsporidia species are obligatory intracellular agents that can infect all major animal groups including mammals, birds, fishes and insects. Whereas worldwide human infection reports are increasing, the cognition of sources of infection particularly zoonotic transmission could be helpful. We aimed to detect zoonotic microsporidia spore in fecal samples from some animals with close – contact to human.
Methods: Overall, 142 fecal samples were collected from animals with closed-contact to human, during 2012-2013. Trichrome – blue staining were performed and DNA was then extracted from samples, identified positive, microscopically. Nested PCR was also carried out with primers targeting SSU rRNA gene and PCR products were sequenced.
Results: From 142 stool samples, microsporidia spores have been observed microscopically in 15 (10.56%) samples. En. cuniculi was found in the faces of 3 (15%) small white mice and 1 (10%) laboratory rabbits(totally 2.81%). Moreover, E. bieneusi was detected in 3 (10%) samples of sheep, 2 (5.12%) cattle, 1 (10%) rabbit, 3 (11.53%) cats and 2 (11.76%) ownership dogs (totally 7.74%). Phylogenetic analysis showed interesting data. This is the first study in Iran, which identified E. bieneusi and En. Cuniculi in fecal samples of laboratory animals with close – contact to human as well as domesticated animal and analyzed them in phylogenetic tree.
Conclusion:
E. bieneusi is the most prevalent microsporidia species in animals. Our results can also alert us about potentially zoonotic transmission of microsporidiosis.Key Words: Laboratory animals, Enterocytozoon bieneusi, Encephalitozoon cuniculi, Zoonotic transmission 相似文献
62.
Shirzad GHOLAMI Farhang BABAMAHMOODI Rohallah ABEDIAN Mehdi SHARIF Abbas SHAHBAZI Abdolsattar PAGHEH Mahdi FAKHAR 《Iranian Journal of Parasitology》2015,10(1):110-115
Background:
Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern areas. The aim of present study was to identify Trichostrongylus spp. among human population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran.Methods:
Overall, 33 positive fecal specimens were randomly sampled and examined. PCR amplification of ITS2-rDNA region was performed on the isolated egg and then a restriction fragment length polymorphism (RFLP) profile was considered to discriminate of Trichostrongylus spp.Results:
A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evidence of both human T. colubriformis and T. axei infections in North of Iran.Conclusion:
T. colubriformis was the probable most common zoonotic species causing human trichostrongylosis infection in the area 相似文献63.
Khalil Ghasemi Falavarjani Joobin Khadamy Arezoo Karimi Moghaddam Nasser Karimi Mehdi Modarres 《Saudi Journal of Ophthalmology》2015,29(4):270-273
Purpose
To evaluate the short-term results of sub-tenon’s injection of bevacizumab in patients with clinically significant macular edema (CSME).Methods
In this prospective non-comparative interventional case series, sub-tenon’s injection of 2.5 mg/0.1 ml bevacizumab was performed for eyes with CSME. Macular thickness and best corrected visual acuity measurements were performed before and one month after injections.Results
Nineteen eyes of twelve patients with a mean age of 59.8 ± 5.7 years were evaluated. Thirteen eyes (68.4%) had center-involving macular edema. No significant difference was observed between pre- and post-injection central subfield retinal thickness measurements (P = 0.3). Central subfield thickness measurements improved or remained unchanged in 13 eyes (68.4%). Baseline BCVA of 0.48 ± 0.35 LogMAR improved to 0.36 ± 0.26 LogMAR after injection (P = 0.01). Improvement of >2 lines in BCVA was found in 5 eyes (26.3%), and no eye lost >2 lines of BCVA. No complication associated with sub-tenon’s injection was observed.Conclusion
Sub-tenon’s injection of bevacizumab resulted in significant short-term visual improvement in eyes with CSME. Retinal thickness changes were not significant. 相似文献64.
Jila Yavarian Farshid Rezaei Azadeh Shadab Mahmood Soroush Mohammad Mehdi Gooya Talat Mokhtari Azad 《Emerging infectious diseases》2015,21(2):362-364
During January 2013–August 2014, a total of 1,800 patients in Iran who had respiratory illness were tested for Middle East respiratory syndrome coronavirus. A cluster of 5 cases occurred in Kerman Province during May–July 2014, but virus transmission routes for some infections were unclear. 相似文献
65.
Razavi Yasaman Keyhanfar Fariborz Haghparast Abbas Shabani Ronak Mehdizadeh Mehdi 《Metabolic brain disease》2021,36(6):1381-1390
Metabolic Brain Disease - Chronic methamphetamine (meth) abuse can lead to certain deficits in the hippocampal function by affecting the hippocampal neurogenesis and plasticity. To determine... 相似文献
66.
Fateme Mezginejad Mohammad Hossein Mohammadi Parinaz khadem Mehdi Allahbakhshian Farsani 《Indian journal of hematology & blood transfusion》2021,37(2):249
LKB1 is a significant tumor suppressor and epigenetic regulator playing a vital role in different types of cancers. SHMT1 and GLDC are two critical genes of the epigenetic pathway influenced by LKB1. As epigenetic is the major cause of AML pathogenesis, this study aimed at investigating LKB1, SHMT1, and GLDC gene expression levels in acute myeloid leukemia patients. The present study was conducted on LKB1, SHMT1, and GLDC gene expression levels in 60 de novo AML samples and 30 normal controls using real-time RT-PCR. The results showed that LKB1 and SHMT1 have respectively a significantly lower (P < 0.05) and higher (P < 0.05) expression level than that of normal controls. Furthermore, the correlation between LKB1 with SHMT1 and GLDC was significant and positive (P value: 0.015, r: 0.299). Positive findings confirm that metabolic pathways alongside the LKB1 association drive the epigenetic axis and its substrate production. Therefore, it can be concluded that the newly-discovered pathway in the pathogenesis of this disease provides new insights into the design of therapeutic targets. 相似文献
67.
Sport Sciences for Health - Extrinsic and intrinsic foot muscles actively support the foot’s arches, particularly the medial longitudinal arch. Previous studies have focused on strengthening... 相似文献
68.
Brkić Hrvoje Galić Ivan Vodanović Marin Dumančić Jelena Mehdi Fuad Anić Milošević Sandra 《International journal of legal medicine》2022,136(6):1685-1696
International Journal of Legal Medicine - This study aimed to evaluate the accuracy and precision of the Cameriere European formula, Demirjian, Haavikko, and Willems methods for estimating dental... 相似文献
69.
Kihara Eunice Galic Ivan Nyamunga Donna Mehdi Fuad Velandia Palacio Luz Andrea Cameriere Roberto 《International journal of legal medicine》2022,136(5):1495-1506
International Journal of Legal Medicine - This study aimed to validate the accuracy of five different formulas based on the Cameriere method for age estimation in Kenyan children. We analyzed... 相似文献
70.