全文获取类型
收费全文 | 24003篇 |
免费 | 2469篇 |
国内免费 | 105篇 |
专业分类
耳鼻咽喉 | 196篇 |
儿科学 | 910篇 |
妇产科学 | 651篇 |
基础医学 | 3413篇 |
口腔科学 | 371篇 |
临床医学 | 3466篇 |
内科学 | 5180篇 |
皮肤病学 | 575篇 |
神经病学 | 2498篇 |
特种医学 | 556篇 |
外国民族医学 | 4篇 |
外科学 | 2342篇 |
综合类 | 211篇 |
一般理论 | 5篇 |
预防医学 | 2411篇 |
眼科学 | 300篇 |
药学 | 1568篇 |
中国医学 | 19篇 |
肿瘤学 | 1901篇 |
出版年
2023年 | 244篇 |
2022年 | 377篇 |
2021年 | 762篇 |
2020年 | 607篇 |
2019年 | 917篇 |
2018年 | 1008篇 |
2017年 | 787篇 |
2016年 | 836篇 |
2015年 | 884篇 |
2014年 | 1068篇 |
2013年 | 1452篇 |
2012年 | 1847篇 |
2011年 | 1755篇 |
2010年 | 1123篇 |
2009年 | 1001篇 |
2008年 | 1503篇 |
2007年 | 1485篇 |
2006年 | 1374篇 |
2005年 | 1287篇 |
2004年 | 1213篇 |
2003年 | 1081篇 |
2002年 | 1002篇 |
2001年 | 216篇 |
2000年 | 196篇 |
1999年 | 215篇 |
1998年 | 240篇 |
1997年 | 189篇 |
1996年 | 148篇 |
1995年 | 148篇 |
1994年 | 128篇 |
1993年 | 110篇 |
1992年 | 83篇 |
1991年 | 91篇 |
1990年 | 87篇 |
1989年 | 76篇 |
1988年 | 68篇 |
1987年 | 50篇 |
1986年 | 64篇 |
1985年 | 56篇 |
1984年 | 61篇 |
1983年 | 61篇 |
1982年 | 69篇 |
1981年 | 69篇 |
1980年 | 41篇 |
1979年 | 28篇 |
1978年 | 35篇 |
1977年 | 42篇 |
1976年 | 50篇 |
1975年 | 38篇 |
1974年 | 38篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
Anne‐Marie Lamhonwah Simon E. Olpin Rodney J. Pollitt Christine Vianey‐Saban Priscille Divry Nathalie Guffon Guy T. N. Besley Russell Onizuka Linda J. De Meirleir Ljerka Cvitanovic‐Sojat Ivo Baric Carlo Dionisi‐Vici Ksenija Fumic Miljenka Maradin Ingrid Tein 《American journal of medical genetics. Part A》2002,111(3):271-284
Primary systemic carnitine deficiency or carnitine uptake defect (OMIM 212140) is a potentially lethal, autosomal recessive disorder characterized by progressive infantile‐onset cardiomyopathy, weakness, and recurrent hypoglycemic hypoketotic encephalopathy, which is highly responsive to L ‐carnitine therapy. Molecular analysis of the SLC22A5 (OCTN2) gene, encoding the high‐affinity carnitine transporter, was done in 11 affected individuals by direct nucleotide sequencing of polymerase chain reaction products from all 10 exons. Carnitine uptake (at Km of 5 μM) in cultured skin fibroblasts ranged from 1% to 20% of normal controls. Eleven mutations (delF23, N32S, and one 11‐bp duplication in exon 1; R169W in exon 3; a donor splice mutation [IVS3+1 G > A] in intron 3; frameshift mutations in exons 5 and 6; Y401X in exon 7; T440M, T468R and S470F in exon 8) are described. There was no correlation between residual uptake and severity of clinical presentation, suggesting that the wide phenotypic variability is likely related to exogenous stressors exacerbating carnitine deficiency. Most importantly, strict compliance with carnitine from birth appears to prevent the phenotype. © 2002 Wiley‐Liss, Inc. 相似文献
102.
Andre Tehe Chantal Maurice Debra L Hanson Marie Y Borget Nadine Abiola Matthieu Maran Daniel Yavo Zuzana Tomasik Jürg B?ni J?rg Schüpbach John N Nkengasong 《Journal of clinical virology》2006,37(3):199-205
BACKGROUND: Quantification of HIV-1 RNA remains difficult to implement in Africa. Simple and inexpensive tests for antiretroviral treatment (ART) monitoring are needed. OBJECTIVE: To evaluate an HIV-1 p24 ELISA, which combines efficient virus disruption, heat-denaturation and signal amplification, in a West African setting. STUDY DESIGN: Eighty-six HIV-1 infected patients from Abidjan, C?te d'Ivoire, were tested for p24, HIV-1 RNA, and CD4+ count at baseline, and twice within 8 months after ART initiation. RESULTS: All patients responded to ART with a minimal HIV-1 RNA drop of 0.5 log(10) at first follow-up. Forty-one (47.7%) then rebounded >0.5 log(10) or persisted above 1000 copies/mL by week 24. The predicted baseline concentration of p24 corresponding to 100,000 copies/mL of HIV-1 RNA, above which ART is recommended, was 4546 fg/mL (95% confidence interval 3148-6566). A prediction model of virologic failure, occurring after an initial response to ART, correctly classified 84% of patients using baseline p24, p24 change on therapy, and achievement of undetectable p24 as explanatory variables. The model and further bootstrap evaluation suggested a good ability to discriminate between sustained or failing virologic response to ART. CONCLUSION: HIV-1 p24 and RNA based-ART monitoring in a low-resource country dominated by HIV-1 CRF02 AG appeared comparable. 相似文献
103.
Tsuchida H Björnholm M Fernström M Galuska D Johansson P Wallberg-Henriksson H Zierath JR Lake S Krook A 《Pflügers Archiv : European journal of physiology》2002,445(1):25-31
The gene of the p85alpha regulatory subunit of phosphatidylinositol (PI) 3-kinase gives rise to several splice variants. We hypothesized that the expression of p85alpha splice variants may be altered in skeletal muscle from subjects with type 2 diabetes mellitus. Skeletal muscle biopsies were obtained from nine type 2 diabetic and eight healthy men, matched for age, body mass index (BMI) and physical fitness. PI 3-kinase activity in skeletal muscle following in vitro insulin stimulation was reduced in subjects with type 2 diabetes. p85alpha mRNA was elevated fourfold in type 2 diabetic as compared to healthy control subjects ( P<0.05). p85alpha mRNA abundance was positively correlated with plasma insulin concentration ( P<0.01) and serum glucose concentration ( P<0.01). Despite this, protein levels of p85alpha, p55alpha, and the novel human p50alpha were not altered in type 2 diabetic subjects. Thus, although gene expression of full-length p85alpha is increased in skeletal muscle from type 2 diabetics, this is not reflected by increased protein levels. Therefore, defects in PI 3-kinase activity are likely due to impaired activation of the enzyme rather than changes in protein expression of the isoforms of the regulatory subunit. 相似文献
104.
Bernard Boutevin Mohammed Khamlichi Jean Marie Lusinchi Jean-Jaques Robin 《Macromolecular chemistry and physics.》1997,198(2):605-617
α-Hydroxy and α-chloroformyl oligomers of poly(butylene terephthalate) (PBT) were prepared in 1,1,2,2-tetrachloroethane by condensing terephthaloyl chloride and 1,4-butanediol and using benzoyl chloride and 4-hydroxybutyl benzoate as chain limitator. The average molecular weight was determined by 1H NMR analysis, and thermal properties were assessed by differential scanning calorimetry (DSC). Preparation of α-thiol oligomers of PBT was also investigated by esterification of α-hydroxy oligomers with thioglycolic acid and using p-toluenesulfonic acid as catalyst. The DSC and TGA analyses pointed out that the introduction of a thiol group by esterification has no influence on the thermal properties of the PBT oligomers. 相似文献
105.
Nicole Bruneau Frédérique Bonnet-Brilhault Marie Gomot Jean-Louis Adrien Catherine Barthélémy 《International journal of psychophysiology》2003,51(1):17-25
The purpose of the present study was to investigate the relations between late auditory evoked potentials (AEPs) recorded at temporal sites (the N1c wave or Tb) and verbal and non-verbal abilities in children with autism. The study was performed in 26 mentally retarded children with autism (AUT) aged 4-8 years (mean age +/- S.E.M. = 71 +/- 2 months; mean verbal and non-verbal developmental quotient +/- S.E.M. = 36 +/- 4 and 48 +/- 3). The stimuli used were 750 Hz tone bursts of 200 ms duration delivered binaurally at different intensity levels (50, 60, 70, 80 dB SPL) with 3-5 s interstimulus intervals. Temporal AEPs were first compared to those of a group of 16 normal children (NOR) in the same age range (mean age +/- S.E.M. = 69 +/- 3 months). We then focused on the AUT group and considered relations between temporal AEPs and the severity of disorders of verbal and non-verbal communication assessed using a behavior rating scale. AEPs recorded on left and right temporal sites were of smaller amplitude in the AUT group than in the NOR group. Increasing intensity-related amplitude was observed on both sides in NOR and only on the right side in AUT. The lack of intensity effect on the left side resulted in a particular pattern of asymmetry at the highest level of intensity (80 dB SPL) with greater N1c amplitude on the right than on the left side (the reverse was found in the NOR group). Electro-clinical correlations indicated that the greater the amplitude of the right temporal N1c responses, the higher the verbal and non-verbal communication abilities. This suggests a developmental reorganization of left-right hemisphere functions in autism, with preferential activation of the right hemisphere for functions usually allocated to the left hemisphere, particularly those involving the secondary auditory areas situated on the lateral surface of the superior temporal gyrus where the N1c/Tb wave is generated. 相似文献
106.
Chatelain D Cordonnier C Brevet M Petit J Sevestre H 《Annals of diagnostic pathology》2005,9(4):231-233
Cholesterol crystal embolization (CCE) is a severe systemic disorder caused by vascular migration of cholesterol crystals originating from ulcerative atherosclerotic plaques located in large arteries. We report 2 cases of CCE diagnosed on bladder transurethral resection in 2 men aged 94 and 72 years. Both patients had atherosclerosis disease. One patient had been treated by heparin 1 month before for pulmonary embolism and the other had had a coronary angiography and bypass graft surgery 5 months before for silent myocardial infarction. One patient presented with hematuria and the other with acute renal failure. Cystoscopy showed multiple papillary tumors of the bladder wall. Bladder transurethral resections showed transitional cell carcinoma with cholesterol crystals occluding the lumen of small arterioles in the submucosa. Eight cases of CCE in the bladder wall have been reported in the literature in 3 women and 5 men aged 56 to 79 years. Cholesterol crystal embolization is often discovered in the bladder wall on necropsy specimens. Only 2 cases have been fortuitously discovered on bladder transurethral resection performed for transitional cell carcinoma. Cholesterol crystal embolization in the bladder wall is often a marker of severe disease although the evolution is quite favorable in our patients, still alive 1 and 2 years after diagnosis. 相似文献
107.
108.
Hudson BI Wendt T Bucciarelli LG Rong LL Naka Y Yan SF Schmidt AM 《Antioxidants & redox signaling》2005,7(11-12):1588-1600
The major consequence of long-term diabetes is the increased incidence of disease of the vasculature. Of the underlying mechanisms leading to disease, the accumulation of advanced glycation end products (AGEs), resulting from the associated hyperglycemia, is the most convincing. Interaction of AGEs with their receptor, RAGE, activates numerous signaling pathways leading to activation of proinflammatory and procoagulatory genes. Studies in rodent models of macro- and microvascular disease have demonstrated that blockade of RAGE can prevent development of disease. These observations highlight RAGE as a therapeutic target for treatment of diabetic vascular disease. 相似文献
109.
Amir?R?RazaviEmail author Hans?Gill Olle?St?l Marie?Sundquist Sten?Thorstenson Hans??hlfeldt Nosrat?Shahsavar the South-East Swedish Breast Cancer Study Group 《BMC medical informatics and decision making》2005,5(1):29
Background
A common approach in exploring register data is to find relationships between outcomes and predictors by using multiple regression analysis (MRA). If there is more than one outcome variable, the analysis must then be repeated, and the results combined in some arbitrary fashion. In contrast, Canonical Correlation Analysis (CCA) has the ability to analyze multiple outcomes at the same time. 相似文献110.
Dynamic responses of oxygen uptake at the onset and end of moderate and heavy exercise in trained subjects. 总被引:1,自引:0,他引:1
Christophe Cleuziou Stéphane Perry Fabio Borrani Anne Marie Lecoq Robin Candau Daniel Courteix Philippe Obert 《Revue canadienne de physiologie appliquée》2004,29(1):32-44
Inconsistencies about dynamic asymmetry between the on- and off-transient responses in VO2 are found in the literature. Therefore the purpose of this study was to examine VO2 on- and off-transients during moderate- and heavy-intensity cycling exercise in trained subjects. Ten men underwent an initial incremental test for the estimation of ventilatory threshold (VT) and, on different days, two bouts of square-wave exercise at moderate (VT) intensities. VO2 kinetics in exercise and recovery were better described by a single exponential model (VT). For moderate exercise, we found a symmetry of VO2 kinetics between the on- and off-transients (i.e., fundamental component), consistent with a system manifesting linear control dynamics. For heavy exercise, a slow component superimposed on the fundamental phase was expressed in both the exercise and recovery, with similar parameter estimates. But the on-transient values of the time constant were appreciably faster than the associated off-transient, and independent of the work rate imposed (VT). Our results do not support a dynamically linear system model of VO2 during cycling exercise in the heavy-intensity domain. 相似文献