创伤性脑损伤诱导海马细胞凋亡模型的建立

目的 建立用于研究创伤性脑损伤诱导海马细胞凋亡的模型。方法 Ｗｉｓｔａｒ大鼠单侧大脑皮质承受不同高度（２、５、和１０ｃｍ）下落的１０ｇ重锤打击（皮质压缩幅度３ｍｍ）,致轻、中、重度脑损伤。观查１￣７ｄ脑含水量,组织病理学变化及原位末端标记法（ＴＵＥＮＬ）检测伤侧海马凋亡细胞的变化。结果 伤侧皮质和海马损伤程度与重锤下落的高度有关,轻、中度损伤各时相点脑含水量无明显变化,重度损伤组３￣７ｄ脑含水量显     

足月妊娠米索前列醇引产效果的观察

目的与方法 :通过足月妊娠孕妇采用阴道后穹窿置米索前列醇引产观察对母婴的影响 ,并设静点催产素者为对照组。结果 :应用米索前列醇组在引产有效率及降低剖宫产率方面均优于催产素组 ,而对新生儿的影响与催产素组无明显差别。结论 :足月妊娠米索前列醇引产是一种安全有效的方法     

综合医院确诊的39例肺结核患者转诊结果随访调查

目的;了解并评价综合医院内科确诊的活动性肺结核病例转诊结果。方法：对我院呼吸内科１９９５年８月－１９９８年８月确认的３９例活动性肺结核患者（不包括结核性胸膜炎）转往结构防治专业机构的结果进行信访和电话随访。结果：仍的３９例活动性肺结核患者中有３５例在确诊后１周内结核病接受０．５ａ－１ａ的全地化疗,３３例获得良好效果,２例死亡,死因分别为肺间质纤维化合并感染肺心病及呼吸衰竭。３例慢性纤维空洞型肺结核     

POLYMORPHISM OF ANGIOTENSIN-CONVERTING ENZYME GENE AND GENETIC SUSCEPTIBILITY TO ASTHMA WITH FAMILIAL AGGREGATION

Objective. Angiotensin-converting enzyme (ACE) plays a key role in the metabolism of angiotensin Ⅱ (AT Ⅱ) and inactivation of bradykinins and tachykinins, which are potent bronchialconstrictors and mediators of inflammation asthma, and ACE is heavily expressed in the lungs. An insertion-deletion (D/I) polymorphism of ACE gene has been shown to be associated with levels of ACE. We investigate whether the polymorphism of ACE gene is associated with asthma and bronchial responsiveness.Methods. A case-control study was carried out in 50 asthmatics, 7 families with at least 2 asthmatic individuals, and 50 healthy subjects. The insertion/deletion (I/D) polymorphism of ACE gene was amplified by polymerase chain reaction (PCR). Methacholine brocho-provocation and pulmonary function tests were performed in all asthmatics. Results. There was an higher gene frequency of DD genotype of ACE gene in asthmatic subjects and families individuals compared with healthy subjects (46%, 53% vs 16%, P＜0.05; odd ratio 4.98). Anhigher prevalence of DD genotype of ACE was in patients with bronchial hyperresposiveness (BHR) (67%vs 33%, P＜0.05; odd ratio 3.8). Accordingly, the mean values of FEV1% and FEV1/FVC were higher in asthmatics carrying non-DD alleles than patients with DD genotype (73.78% vs 56.56%, P＜0.05; 79.19% vs 69.29%, P＜0.05, respectively).Conclusion. These results suggested that DD allele of ACE genotype was significantly involved in genetic susceptibility to asthma. DD genotype of ACE might be a risk factor for the degree of airway obstruction, it could also be implicated in pathogenesis of bronchial hyperresponsiveness.     

旅游区院前急救模式探讨

目的：探讨旅游区院前急救模式。方法：对２６６例院急救病例进行分类处理。结果：占前三位疾病是外伤、中毒、心脑血管疾病,除８例死亡外,全部转送院内诊治。结论：院前急救是急诊医学的关键环节,关系到院内进一步急救的抢救成功率。     

特洛细胞在不同组织中的分子标记研究现状

<正>特洛细胞（telocytes,TCs）是一种新发现的间充质来源的间质细胞,形态结构具有特异性和多样性,目前认为TCs可能对维持组织稳态和损伤修复有潜在的作用,参与不同疾病的发生发展[1]。TCs于2005年被L.M. Popescu教授等[2]在电镜下发现,认为其与Cajal间质细胞（interstitial cells of Cajal,ICCs）结构类似,遂将其命名为Cajal样间质细胞（interstitial Cajallike cells,ICLCs）。随着显微镜技术的发展及对TCs的深入研究,人们发现此类细胞与ICCs在分子标记上存在明显不同,     

Chevron截骨术对外翻足关节接触特征影响的生物力学研究

目的 探究Chevron截骨术截骨远端外侧位移距离对外翻足关节接触特征的影响,为临床上选择合适的位移距离提供参考依据。方法 测量踝关节中立位状态下正常足、外翻足及外翻足Chevron截骨术截骨远端分别向外侧位移2.0、4.0、6.0 mm后前足、中足和后足各关节接触力、峰值压强和接触面积,并对结果进行分析。结果 与正常足相比,外翻足第1跖楔关节（t=-3.33, P=0.02）、跟骰关节（t=-2.74, P=0.03）和距下关节（后关节面）（t=-2.89, P=0.03）的接触力显著增高;外翻足距舟关节（t=-2.73, P=0.03）与跟骰关节（t=-2.74, P=0.03）的峰值压强显著增高;行Chevron截骨术后,随着截骨远端向外侧位移距离的增加,外翻足第1跖楔关节和跟骰关节的接触力逐渐减小;外翻足距舟关节和跟骰关节的峰值压强逐渐减小。结论 中度外翻足行Chevron截骨术后,当第1跖骨截骨远端向外侧位移6 mm时能有效恢复部分关节间力的分布,且能缓解部分关节局部应力集中现象。     

Synthesis and Study of Copolymers Bearing Two Coumarin Side Groups in Monomer Unit: Toward Photoalignment of Liquid Crystals

Photodimerization of coumarin derivatives in photoactive polymers under linearly polarized UV (LPUV) light irradiation is a photochemical reaction often used to prepare the surface alignment layer that can orient liquid crystals (LCs) due to the anisotropic interfacial interactions. Herein, the synthesis of a series of novel coumarin-containing copolymers and their use for the photoalignment surface layer of LCs is reported. These copolymers have the particular feature of bearing two coumarin side groups in each monomer unit, which is designed to have a high content of the chromophores. The alignment of LCs induced by the photoalignment layers of the coumarin-containing copolymers is investigated. It is found that the photoalignment layers can effectively induce orientation of LC molecules along the direction of the UV light polarization used to prepare the surface layers, with an order parameter of around 0.5 achieved by adjusting the preparation conditions. Moreover, the electro-optical behaviors of LC cells made with the photoalignment layers are investigated, confirming the alignment of LCs without the use of rubbed surfaces. Optically inscribing the photoalignment layer for nonuniform and organized LC alignment is also demonstrated.     

Dengue determinants: Necessities and challenges for universal dengue vaccine development

Dengue illness can range from mild illness to life-threatening haemorrhage. It is an Aedes-borne infectious disease caused by the dengue virus, which has four serotypes. Each serotype acts as an independent infectious agent. The antibodies against one serotype confer homotypic immunity but temporary protection against heterotypic infection. Dengue has become a growing health concern for up to one third of the world's population. Currently, there is no potent anti-dengue medicine, and treatment for severe dengue relies on intravenous fluid management and pain medications. The burden of dengue dramatically increases despite advances in vector control measures. These factors underscore the need for a vaccine. Various dengue vaccine strategies have been demonstrated, that is, live attenuated vaccine, inactivated vaccine, DNA vaccine, subunit vaccine, and viral-vector vaccines, some of which are at the stage of clinical testing. Unfortunately, the forefront candidate vaccine is less than satisfactory, and its performance depends on serostatus and age factors. The lessons from clinical studies depicted ambiguity concerning the efficacy of dengue vaccine. Our study highlighted that viral structural heterogeneity, epitope accessibility, autoimmune complications, genetic variants, genetic diversities, antigen competition, virulence variation, host-pathogen specific interaction, antibody-dependent enhancement, cross-reactive immunity among Flaviviruses, and host-susceptibility determinants not only influence infection outcomes but also hampered successful vaccine development. This review integrates dengue determinants allocated necessities and challenges, which would provide insight for universal dengue vaccine development.     

可注射矿化胶原水凝胶修复大鼠颅骨临界性骨缺损的研究

目的 基于矿化胶原（MC）与海藻酸盐（ALG）,制备一种新型可注射矿化胶原水凝胶（MCA）,研究MCA对颅骨临界性骨缺损的修复作用。方法 将MC粉末以200 mg/mL的比例加入到ALG（C_ALG=15 mg/mL）中制备出矿化胶原水凝胶（MCA）。采用扫描电子显微镜对MCA的交联状态和元素分析情况进行观察。采用L929细胞增殖实验评估MCA的生物安全性,体内降解实验观察MCA降解性和生物相容性。通过大鼠颅骨临界骨缺损模型来验证MCA的骨修复效果。结果 MCA水凝胶经过扫描电子显微镜（SEM）观察后发现水凝胶表面粗糙,其内部Ca/P信号均匀且密集。为了评估MCA的生物安全性,使用MCA浸提液与L929细胞共同孵育后,发现200 mg/mL浸提液实验组的L929细胞增值率为99.95%,毒性评级为1级。同时,将MCA水凝胶注射至大鼠皮下,术后60 d注射部位仍见残留的MCA植入物,而在MCA水凝胶注射到颅骨缺损处的第60天时,MCA则全部降解。最后,观察MCA在颅骨临界骨缺损动物模型中的骨缺损修复效果,实验结果表明MCA水凝胶组的颅骨缺损修复效果优于ALG水凝胶组和对照组。结论 MCA水凝胶具有良好的生物安全性,降解性和促进骨再生的能力,是一种安全可靠的骨修复材料。