中药汤剂在舟曲特大泥石流灾后防疫保健中的应用

目的:了解灾区人群感冒、腹泻的发病状况及对中药汤剂的态度、效果等。方法:采用自制调查问卷、对灾区中药发放及对感冒、腹泻发病情况进行数据统计等方法。结果:8月12日至8月19日腹泻病人和感冒病人呈高发病趋势,从8月20日开始至9月11日随着中药发放人数的增加,发病呈现稳定的下降趋势。调查问卷显示:2113人在"8.8"灾后有36.2%的人得过腹泻,有48.7%的人得过感冒,8.0%的人得过皮肤病,7.8%的人得过抑郁失眠,还有4.3%的人得过其他病;有97.5%的人每天至少喝1次中药汤剂;65.1%的人对中药在灾后防疫中大量应用持非常欢迎的态度,有32.3%的人持积极配合的态度,只有2.6%的人持无所谓的态度;有89.7%的人认为中药对感冒和腹泻绝对有预防作用,只有10.3%的人认为说不上有还是没有;有76.7%的人认为今后的疾病防疫中中医中药应该广泛应用,23.3%的人认为应配合其他措施应用。结论:中药汤剂可降低感冒、腹泻的发病率,在舟曲灾后受到广泛欢迎,在今后的疾病防疫中,应该广泛推广应用。     

全口义齿固位及稳定影响因素探讨

目的探讨提高全口义齿固位及稳定的方法。方法通过对患者在试戴及应用全口义齿出现的固位稳定不良现象进行检查分析,寻找出相应的改进方法。结果全口义齿固位不良主要原因是未建立平衡,其次是制作误差及口腔解剖条件差。结论提高全口义齿固位稳定性的有效方法是确保全口义齿良好的咬合关系,提高操作精确度,以弥补口腔条件差的缺陷。     

喷雾冷冻干燥对家蝇幼虫活性成分抗菌及抗炎活性影响

以人工饲养繁殖的家蝇（Musca）3龄幼虫为材料,探讨喷雾冷冻干燥对家蝇幼虫活性提取物抗菌及抗炎活性的影响。结果表明,经喷雾冷冻干燥处理的家蝇幼虫活性提取物对金黄色葡萄球菌和大肠埃希菌具有有效抑制作用;对LPS诱导的巨噬细胞炎症因子TNF—α和IL-6表达具有明显的抑制作用。     

试论小青龙汤在耳鼻咽喉科的临床应用

小青龙汤方出自张仲景《伤寒论》一书,全方由麻黄、桂枝、半夏、芍药、干姜、细辛、五味子、炙甘草组成,主治表寒内饮、痰多而稀、舌苔白滑、脉浮紧等症.因本方配伍精炼,用药严谨,后世延用至今,每每称道.本人在临床中选用小青龙汤方治疗耳鼻咽喉科相关疾病,取得较好疗效,简述体会如下.     

血脂异常中医证候及与代谢综合征相关性的研究

通过对600例血脂异常患者中医证侯的研究,总结了血脂异常中医证候分布的规律,确认血脂异常患者的病机是以本虚为主,脾虚和肾虚是血脂异常发病的主要病理基础,痰浊和瘀血也是影响血脂异常中医证候的重要因素,建议在治疗时应以补脾益肾为主要治疗原则。并首次在血脂异常的辨证中提出了＂无证候可辨＂的概念。通过对血脂异常中医证候与代谢综合征（MS）相关性的研究发现血脂异常中医证候的产生与MS及其组分中的超重或肥胖及高血压密切相关,提出MS及其各组分的存在是引起血脂异常中医虚证的重要因素,阐明了应用辨证论治的方法在治疗血脂异常的同时可以改善MS,还可以提高患者的生活质量,因而优于单纯的降脂治疗。     

羌活化学成分研究Ⅲ羌活石油醚提取部分化学成分的分离鉴定

经硅胶柱层析,制备薄板层析分离,化学分析和光谱鉴定,从羌活的石油醚提取部分分离并鉴定了β-谷甾醇、油酸、亚油酸、哥伦比亚甙元、娠烯醇酮和阿魏酸6种化学成分。娠烯醇酮为首次从本属植物中分离鉴定。     

痹颗粒对RA-MsPGN大鼠肾组织PTK活性的影响

目的探讨痹颗粒对RA-MsPGN大鼠模型肾组织PTK活性的影响,治疗RA-MsPGN的作用机制。方法按照Phospho Safe TM ExtR Action Reagent试剂盒提取蛋白质,按照K-LISA PTK Screening Kit试剂盒测定蛋白酪氨酸激酶(PTK)活性,并根据检测试剂盒提供的标准品直线回归方程计算PTKs的含量。结果RA-MsPGN模型大鼠与正常组相比肾组织PTK含量显著升高(P<0.001),雷公藤多苷片和痹颗粒各剂量组均能显著降低大鼠肾组织PTK含量(P<0.05或0.01)。结论痹颗粒对RA-MsPGN大鼠肾组织PTK活性有明显抑制作用,其治疗作用有可能是通过对PTK的作用而实现的。     

Inhibitory effect of Ginkgo biloba extract on hyperhomocysteinemia-induced intimal thickening in rabbit abdominal aorta after balloon injury

Ginkgo biloba extract (GBE) has been widely used to treat cardiovascular and cerebrovascular disorders. Hyperhomocysteinemia (Hhcy) is associated with the risk of atherosclerosis and restenosis after angioplasty. The objective of this study was to investigate whether GBE could attenuate the Hhcy-induced intimal thickening after balloon injury in rabbit abdominal aorta. It was observed in this study that GBE could decrease the neointima area (NA) and the ratio of the neointima area to the media area (NA/MA), down-regulate the mRNA expression of matrix metalloproteinase-9 (MMP-9) and up-regulate the protein expression of p21 (WAF1/CIP1) (p21). It suggests that GBE can reverse the Hhcy-induced neointima formation in rabbits following balloon injury, and the suppressive effect of GBE on the migration and proliferation of vascular smooth muscle cells (VSMCs) may contribute to its actions.     

Giganteaside D induces ROS-mediated apoptosis in human hepatocellular carcinoma cells through the MAPK pathway

Purpose

Every year, almost one million individuals are diagnosed with hepatocellular carcinoma (HCC) worldwide and more than 690,000 patients die of it. At present, most therapeutic anti-HCC agents are not effective, which is due to the appearance of chemo-resistance and/or toxic side effects. Therefore, it is imperative to find novel more effective anti-HCC agents. Here, we evaluated the effect of giganteaside D (GD), an oleanolic acid saponin from P. scabiosaefolia, on the growth and apoptosis of HCC cells.

Methods and results

Using MTT and clonogenic assays, we found that GD exhibited a significant growth inhibitory effect on the HCC-derived cell lines HepG2 and Bel-7402. In addition, we found that GD induced mitochondria-mediated apoptosis in these HCC-derived cells, as indicated by a decreased mitochondrial potential, activation of Caspase-9 and Caspase-3, cleavage of PARP and release of Cytochrome C from the mitochondria. Besides, we found that GD stimulated the generation of reactive oxygen species (ROS) and that blockage of ROS attenuated the GD-induced mitochondria-mediated apoptosis. Additionally, we found that GD treatment led to a decrease in phosphorylated Erk (p-Erk) and triggered the generation of p-JNK, both components of the mitogen-activated protein kinase (MAPK) signaling pathway. Inhibition of Erk or JNK by specific inhibitors or siRNAs augmented or attenuated the cytotoxic and apoptotic effects of GD.

Conclusions

From our results we conclude that GD can induce ROS-mediated apoptosis in HCC-derived cells through the MAPK pathway. This observation may open up avenues to explore the future use of GD as a HCC chemotherapeutic agent.

     

维生素C对顺铂杀伤卵巢癌细胞的影响

目的:探讨维生素C 是否能影响顺铂杀伤卵巢癌细胞株OVCAR5、SKOV3、CAOV3,并对其作用机制进行初探。方法:使用梯度浓度维生素C及顺铂处理卵巢癌细胞株,显微镜下观测细胞的形态学变化;使用ATP-TCA方法检测细胞生长抑制情况。选择对维生素C敏感的卵巢癌细胞株。氧化敏感探针DCFH-DA标记细胞 ,检测不同浓度维生素C处理不同时间后细胞内过氧化氢(H2O2 )水平。结果:三株卵巢癌细胞维生素C处理的敏感度不同,但总体呈现出随着维生素C浓度的增加,细胞受到抑制作用增加,维生素C对于顺铂杀伤细胞的作用具有辅助加强效应,该作用呈现剂量依赖效应。其中较低浓度维生素C处理OVCAR5细胞15min各组胞内H2O2 水平相比对照组稍高,当处理浓度为5 000μmol/L时细胞H2O2 水平明显升高,达到164%。培养30min后,荧光强度显示细胞内H2O2 水平比15min时升高更明显。不同浓度组分别达到对照组的94%、106%、122%、188%,培养60min后检测荧光强度发现各给药组H2O2 水平均已低于对照组。12h后检测到细胞内的H2O2 水平处于更低水平,各组均未达到对照组的50%。结论:较高浓度维生素C能增强顺铂杀伤卵巢癌细胞株的能力,并呈现一定的剂量依赖性。该结果可能由于维生素 C通过自氧化机制产生过氧化氢促进卵巢癌细胞凋亡。