Characterization of <Emphasis Type="Italic">p</Emphasis>24 Gene of <Emphasis Type="Italic">Spodoptera litura</Emphasis> Multicapsid Nucleopolyhedrovirus

Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) p24 gene is 753 bp long, potentially encoding 244 amino acids with a predicted molecular weight of 27.3 kDa. Homology analysis indicated that SpltMNPV P24 has 20–36% amino acid identity with that of other known baculoviruses. RT-PCR results showed that the p24 gene is transcribed actively at the late stage of infection and the mRNA start site was mapped within a consensus baculovirus late promoter sequence (ATAAG). Western blot analysis of extracts from SpltMNPV-infected S. litura cells detected a specific 28 kDa protein, and this protein was not N-glycosylated. Structural localization revealed that SpltMNPV P24 was associated with the nucleocapsid of occlusion-derived virus (ODV) as a complex form of 83 kDa.     

Response rates to a questionnaire 26 years after baseline examination with minimal interim participant contact and baseline differences between respondents and nonrespondents

Research on response rates to surveys mailed to study participants decades after baseline examination, with minimal interim contact, is limited. This paper documents response rates to a 26-year follow-up survey of surviving participants from a large cohort in Illinois and compares baseline characteristics of nonrespondents and respondents. Mortality follow-up of the Chicago Heart Association Detection Project in Industry 1967-1973 cohort involved minimal or no participant contact since baseline. In 1996, a 26-year follow-up questionnaire was mailed to all surviving participants aged 65 years or older. Current addresses were obtained from the Health Care Financing Administration for 96.5 percent of 12,409 participants in our analyses. Total response rates were 59.8 percent and, for participants for whom Health Care Financing Administration addresses were available, 60.8 percent. A higher response rate was obtained for younger recipients, men, Whites, more-educated persons, nonsmokers, and those with a better cardiovascular risk profile at baseline. A graded negative relation was found between number of cardiovascular risk factors at baseline and response rates obtained in 1996. Use of Health Care Financing Administration records as an additional follow-up method and factors that influence response rates are discussed. In conclusion, long-term follow-up of older surviving participants is feasible if current addresses can be obtained from standardized sources.     

Comparison of low risk and higher risk profiles in middle age to frequency and quantity of coronary artery calcium years later

Little is known about the relation of having favorable levels of all major cardiovascular risk factors (low risk [LR]) earlier in life to coronary artery calcium (CAC) later in life. From 2002 to 2003, CAC was compared in participants aged >60 years who were LR (n = 42) with those not LR (n = 39) at baseline (from 1967 to 1973). Despite adverse changes in risk factors, the prevalence of measurable CAC and mean CAC scores were less for LR participants than for non-LR participants (60% vs 77%, p = 0.09, and 217 vs 443, p = 0.05, respectively).     

Monaural middle ear destruction in juvenile and adult mice: effects on responses to sound direction in the inferior colliculus ipsilateral to the intact ear

This study examined the effect of monaural middle ear destruction on auditory responses to sound direction in the inferior colliculus (IC) of the laboratory mice, Mus musculus. Monaural middle ear destruction was performed on juvenile and adult mice (the experimental mice). Auditory response properties of neurons to ipsilateral and contralateral sounds (I-40 degrees and C-40 degrees ) were examined in the IC ipsilateral to the intact ear 4 weeks later. IC neurons of control mice had higher minimum thresholds (MTs), larger Q(n) (Q(10), Q(30)) values but smaller dynamic ranges at I-40 degrees than at C-40 degrees. These direction-dependent response properties were not observed for IC neurons of experimental juvenile and adult mice. However, Q(n) values of IC neurons were significantly smaller in experimental juvenile than in control and experimental adult mice. Normal tonotopic organization in terms of positive correlation between recording depth and best frequency (BF) was observed in the IC of control and experimental adult mice at both sound directions but not in the IC of experimental juvenile mice. A positive correlation of increasing MT with BF was only observed for IC neurons in control mice but not in both experimental mice. Possible mechanisms for these different response properties are discussed.     

颈动脉切除重建术在头颈肿瘤中的应用

目的 探讨颈动脉切除重建术在累及颈动脉的头颈肿瘤外科治疗中的应用价值.方法 分析7例累及颈动脉的头颈肿瘤患者一期切除肿瘤和颈动脉及颈动脉重建手术的方法、效果及5术后并发症,3例为ShamblinⅢ型颈动脉体瘤,2例为喉癌术后复发并颈部淋巴结转移,2例为喉咽癌术后复发或放疗后并颈部淋巴结转移.结果 7例患者均在颈动脉转流下切断颈动脉,肿瘤连同颈动脉整块切除,采用自体大隐静脉或聚四氟乙烯膨体(expanded polytetrafluoroethylene,ePTFE)人工血管重建颈动脉.所有患者术后均未出现中枢神经系统并发症,2例颈动脉体瘤患者出现单侧喉返神经麻痹和霍纳综合征.1例患者因咽瘘导致颈动脉破裂二次行颈动脉重建术;2例患者术后形成咽瘘,其中1例出现肿瘤复发,1例经换药愈合.结论对于累及颈动脉的头颈肿瘤,颈动脉切除重建术有可能降低手术后神经系统并发症发生,改善患者的生活质量.     

以病人为中心 优化门诊流程

通过对医院流程的不断优化,使流程更趋向合理、便捷,从而提高患者的有效就诊率,体现医院真诚的为病人服务的理念。     

22例服药自杀患儿的心理状态分析与护理

儿童轻生现象通过近年来的临床资料统计有逐年上升趋势,这与儿童耐受力低、反应性强、对各种不良刺激引起情绪反应极为敏感的心理特点有关。本文报道1995年4月～1998年5月收治22例服药自杀患儿,对其自杀原因、患儿的心理状态及心理疏导方法做了较详细的阐述。提出对自杀患儿护理的要点应在于从心理社会的角度去认识,同时创造一个良好的社会及家庭环境,提高儿童心理素质,是减少儿童轻生的关键。     

涎腺恶性肌上皮瘤的免疫组织化学研究

目的　探讨涎腺恶性肌上皮瘤的临床病理特征及生物学行为。方法　采用组织病理学和免疫组化方法对 2 2例恶性肌上皮瘤进行分析。结果　好发于腮腺和腭腺 ;男性多见 (男女之比 2 3∶1) ;中位年龄4 9岁。病理特征为透明细胞型和上皮样细胞型多见 ,呈巢索状分布 ;细胞异型性明显 ,有病理性核分裂像 ;广泛浸润周围组织。免疫组化染色Actin、Ck 18、EGFR、TGFβ均呈明显的强阳性表达 ;PCNA阳性表达的平均增殖指数是 2 2 8%± 6 93,以透明细胞型的PI值为最高 ;癌基因C erbB 2、抑癌基因P53分别呈 63 6%和31 8%的阳性表达 ,各型的表达也以透明细胞型为最高。结论　恶性肌上皮瘤是一种上皮性肿瘤 ,其亚型透明细胞型恶性程度较高。C erbB 2、P53和PCNA的异常表达及协同作用在恶性肌上皮瘤的发生发展中起重要作用。     

兔网织红细胞胞质因子对小鼠骨髓瘤细胞恶性调控的研究

以无细胞核的兔网织红细胞与小鼠骨髓瘤细胞BW(胸腺淋巴肉瘤)及SP2/0(浆细胞瘤)分别进行异种杂交。实验结果表明杂交后形成的二种胞质杂交细胞(BW-RR及SP 2/0-RR)均呈现下列去恶性分化特征;①细胞分裂指数、~3H-TdR标记指数及生长曲线明显下降。其中SP2/0-RR的核分裂活动受到明显的抑制;②在软琼脂培养中不形成集落;③异种移植至裸鼠不长瘤;④染色体数量减少,众数及畸变率下降;⑤出现正常分化的珠蛋白基因产物血红蛋白和HGPRT基因的酶产物,珠蛋白探针分子杂交实验证明有珠蛋白基因的表达。为不同种属红细胞胞质因子调控肿瘤细胞恶性提供了证据。论文对胞质因子调控基因表达、恶性逆转及其在红细胞发生过程中自然去核的物质基础等问题进行了讨论。