盐酸埃他卡林对[~3H]格列本脲与动脉平滑肌K_(ATP)结合和解离动力学过程的影响以及与核苷酸的交互作用

目的　研究盐酸埃他卡林 (Iptakalimhydrochloride,Ipt)对 [3 H]格列本脲 (glibenclamide,Gli)与大鼠血管平滑肌ATP敏感性钾通道 (ATP sensitivepotassiumchannel,KATP)的硫脲受体(Sulfonylureareceptor,SUR2B)结合和解离动力学过程的影响 ,并比较其作用特点与核苷酸类物质的异同点及它们之间的交互效应。方法　KATP拮抗剂 [3 H]Gli与大鼠去内皮主动脉平滑肌特异性结合与解离的动力学试验。结果　 (1 )在浓度为 1 0pmol·L-1 ～ 0 5mmol·L-1 范围内 ,Ipt不能取代SUR2B与 [3 H]Gli2 0 0 3 0 616收稿 ,2 0 0 3 112 5修回 国家新药研究与发展重点项目 ,No 9690 10 10 1;国家“863”计划重大专项 ,No 2 0 0 2AA2Z3 13 7作者简介 :何华美 ,男 ,3 6岁 ,博士 ,副教授。研究方向 :心血管药理学。E mail:longchaoliang @sohu .com ;汪　海 ,男 ,40岁 ,研究员 ,博士生导师。研究方向 :心血管药理和新药。通讯作者。Tel :86 10 6693 2 65 1,Fax:86 10 682 1165 6,E mail:wh @nic.bmi.ac .cn之间的特异性结合。Ipt 1 0 0 μmol·L-1 调节 [3 H]Gli与SUR2B特异性结合的动力学过程的特征为抑制其结合动力学过程 ,使其结合速率减慢 ,结合幅度降低 ;促进其解离动力学过程 ,使其解离速率加快 ,解离幅度增加。 (2 )ATP     

RP-HPLC法同时测定注射用粉末中他唑巴坦和头孢他啶组分的含量

目的建立反相高效液相色谱法用于同时测定他唑巴坦和头孢他啶的含量.方法采用Zorbax 300SB-C18色谱柱, 流动相为甲醇-磷酸盐缓冲液 (pH=5.6),检测波长为220 nm.结果他唑巴坦和头孢他啶分别在0.62-631.8 μg·mL-1和 0.66-677.5 μg·mL-1内呈线性关系,平均加样回收率分别为98.8% - 101.4%和99.1%-100.2%,日内和日间精密度分别为0.2%-1.5%和0.1%-2.6%.结论该法简单、精确、快速、重复性好,可以满足其原料和制剂的质量控制要求.     

国产班布罗的平喘作用

目的　研究国产班布特罗的平喘作用。方法　采用豚鼠整体引喘法及兔肺溢流法。结果　班布特罗 (ig ,1 4、2 8、5 6mg·kg-1)同间羟叔丁肾上腺素作用相似 ,均具有平喘及松驰气管平滑肌的作用。结论　国产班布特罗具有明显的平喘作用     

跨声门癌50例观察

为确立一种原发喉室的跨声门癌，选择肿瘤主体以喉室为中心经全喉切除手术的标本５０例，火棉胶包埋，连续切片观察。结果发现①跨声门癌的肿瘤原发部位在喉室，５０例中有Ｔ２３例。②肿瘤以深层浸润为主占９０％（４５／５０），易侵及声门旁间隙（８２％，４１／５０）和甲状软骨（６４％，３２／５０），临床分期与病理分期不符达４８％（２４／５０）。结论：原发喉室的跨声门癌是声门上型癌的一种特殊类型。     

Trace element levels in drinking water and cognitive function among elderly Chinese

The relation between trace element levels in drinking water and cognitive function was investigated in a population-based study of elderly residents (n = 1,016) in rural China in 1996-1997. Cognitive function was measured using a Chinese translation of the Community Screening Interview for Dementia. A mixed effects model was used to evaluate the effect of each of the elements on cognitive function while adjusting for age, sex, and educational level. Several of the elements examined had a significant effect on cognitive function when they were assessed in a univariate context. However, after adjustment for other elements, many of these results were not significant. There was a significant quadratic effect for calcium and a significant zinc-cadmium interaction. Cognitive function increased with calcium level up to a certain point and then decreased as calcium continued to increase. Zinc showed a positive relation with cognitive function at low cadmium levels but a negative relation at high levels.     

芫花根皮的生药学研究

描述了芫花根皮的药材性状、组织构造及粉末特征，为鉴别芫花根皮提供参考依据。     

EXPRESSION OF EBV LATENT MEMBRANE PROTEIN IN ESOPHAGEAL CARCINOMA AND PARA-CANCEROUS MUCOSA

ＥＸＰＲＥＳＳＩＯＮＯＦＥＢＶＬＡＴＥＮＴＭＥＭＢＲＡＮＥＰＲＯＴＥＩＮＩＮＥＳＯＰＨＡＧＥＡＬＣＡＲＣＩＮＯＭＡＡＮＤＰＡＲＡＣＡＮＣＥＲＯＵＳＭＵＣＯＳＡＷｕＭｉｎｇｙａｏ吴名耀ＬｉａｎｇＹｉｎｇｒｕｉ梁英锐ＷｕＸｉａｎｙｉｎｇ吴贤英Ｄｅｐａｒ...     

ADULT MESENCHYMAL HAMARTOMA OF THE LIVER: A CASE REPORT

ＡＤＵＬＴＭＥＳＥＮＣＨＹＭＡＬＨＡＭＡＲＴＯＭＡＯＦＴＨＥＬＩＶＥＲ：ＡＣＡＳＥＲＥＰＯＲＴＷａｎｇＸｉａｎｇｈｕｉ王湘辉ＣｈａｉＦｕｌｕ柴福录ＤｏｎｇＬｉａｎｇ董亮ＬａｎｚｈｏｕＧｅｎｅｒａｌＨｏｓｐｉｔａｌｏｆＰＬＡ，Ｌａｎｚｈｏｕ７３００５０...