The Potential Roles of Dietary Anthocyanins in Inhibiting Vascular Endothelial Cell Senescence and Preventing Cardiovascular Diseases

Cardiovascular disease (CVD) is a group of diseases affecting the heart and blood vessels and is the leading cause of morbidity and mortality worldwide. Increasingly more evidence has shown that the senescence of vascular endothelial cells is the key to endothelial dysfunction and cardiovascular diseases. Anthocyanin is a type of water-soluble polyphenol pigment and secondary metabolite of plant-based food widely existing in fruits and vegetables. The gut microbiome is involved in the metabolism of anthocyanins and mediates the biological activities of anthocyanins and their metabolites, while anthocyanins also regulate the growth of specific bacteria in the microbiota and promote the proliferation of healthy anaerobic flora. Accumulating studies have shown that anthocyanins have antioxidant, anti-inflammatory, and anti-aging effects. Many animal and in vitro experiments have also proven that anthocyanins have protective effects on cardiovascular-disease-related dysfunction. However, the molecular mechanism of anthocyanin in eliminating aging endothelial cells and preventing cardiovascular diseases is very complex and is not fully understood. In this systematic review, we summarize the metabolism and activities of anthocyanins, as well as their effects on scavenging senescent cells and cardioprotection.     

Effects of organophosphorus pesticides and their ozonation byproducts on gap junctional intercellular communication in rat liver cell line.

The effects of organophosphorus pesticides (OPs), oxons and their ozonation byproducts on gap junctional intercellular communication (GJIC) on cultured BRL cell line were investigated using scrape loading and dye transfer (SL/DT) technique. The neutral red uptake assay was used to identify the non-cytotoxic levels of diazinon, parathion and methyl-parathion applied to GJIC assay. The concentration-dependent inhibition of GJIC was observed over a range of 50-350 mg/l diazinon, parathion and methyl-parathion after 90 min incubation compared with the vehicle control. However, oxons and ozonation byproducts of OPs had no inhibition effect on GJIC at any of the concentrations tested. The inhibition of GJIC by OPs was reversible after removal of the tested pesticides followed by incubation with fresh medium. The present study suggested that the ozonation treatment could be used for the detoxification of drinking water and food crops contaminated with diazinon, parathion and methyl-parathion without formation of GJIC toxicity.     

-组新的N-羟基肉桂酰胺类组蛋白去乙酰化酶抑制剂的合成和初步活性研究

目的 设计合成了一组新的N-羟基肉桂酰胺类衍生物并测定其对组蛋白去乙酰化酶(HDACs)的抑制活性.方法 以阿魏酸为原料,经酯化、Williamson反应、皂化、缩合4步反应合成了一组新的N-羟基肉桂酰胺类衍生物;用组蛋白去乙酰化酶活性检测试剂盒测定该组化合物体外抑酶活性.结果 与结论 合成了11个未见文献报道的N-羟基肉桂酰胺类衍生物,其结构经过1H-NMR、MS、和IR确认.其中化合物5a、5b、5e和5k的体外抑酶活性较强(IC50=2～15μmol·L-1),有可能具有抗肿瘤活性,值得进一步研究.     

Causes and trend of maternal death in a local obstetric unit in Hong Kong

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近年来网络成瘾的病因及治疗学研究进展概述

随着现代科学技术的发展,网络逐渐走进越来越多人的生活。网络就像一把双刃剑,给人们的生活带来快捷的同时,也严重的影响着人们。由此伴生的网络成瘾问题也越来越成为一种显著的社会问题。网络成瘾是一种疾病,然而目前对其研究较多的则是从心理学角度加以认识和干预。文章主要概述了网络成瘾的现状,发病的生物化学机制以及生物医学、心理学、药物、针灸等治疗方法与现状,并对存在的问题和今后的研究方向加以总结和探讨。以对今后的治疗提供参考。     

中西医结合治疗急性胰腺炎伴胃肠功能障碍疗效评价研究

目的探讨中西医结合治疗急性胰腺炎（AP）伴胃肠功能障碍的临床疗效。方法回顾性分析377例AP临床资料,按治疗方法分为中西医结合组和西医组,对比分析2组胃肠功能改善的差异性。结果中西医结合组在腹胀缓解时间、自主排便好转时间、肠鸣音好转时间方面均优于西医组,差异有统计学意义（P〈0.05）。结论中西医结合治疗可显著改善AP患者的胃肠功能。     

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??OBJECTIVE To establish a capillary zone electrophoresis(CZE)-based method for charge heterogeneity analysis of IgG2 monoclonal antibody. METHODS The concentrations of TETA and EACA, pH of separation buffer and temperature of capillary were optimized, to obtain a method which can efficiently separate the charge variants of IgG2 mabs. The reproducibility and repeatability of the optimized CZE method were validated and a battery of mabs were evaluated. RESULTS The separation effect was significantly influenced by TETA/EACA concentration, pH and separation temperature. Through a series of optimization, a CZE method which showed good resolution and precision(RSD of area percentage was below 3.0% was established and the method parameters were as below: 400 mmol??L^-1 EACA/0.05% TETA, pH 6.2, separation under 30 kV voltage at 35 ??. This method was also suitable for IgG1 and IgG4 mabscharge heterogeneity analysis. Based on the specific electrophorogram and migration time, it could also be used as an identification method for monoclonal antibodies. CONCLUSION The optimized CZE method can efficiently separate the charge variants of IgG2 mabs and shows good precision and specificity, which can be used to analyze the charge heterogeneity and identification of monoclonal antibodies.     

Xanthone glycosides from Polygala tenuifolia and their conformational analyses

Seven xanthone glycosides were isolated from the cortexes of Polygala tenuifolia, and their structures were identified as polygalaxanthones VIII-XI (1-4), sibiricoxanthone B (5), 7-O-methylmangiferin (6), and lancerin (7), on the basis of spectroscopic analyses. Compounds 1-4 are new xanthone glycosides, and compounds 4 and 5 exist as rotamers. To explain this phenomenon, conformational analyses were performed on compounds 4 and 5 and other compounds with similar skeletons that were isolated from P. tenuifolia.     

造血干细胞移植治疗恶性血液病的临床观察

目的:观察我院骨髓移植治疗恶性血液病的疗效、探讨移植相关并发症的预防及处理等问题。方法:4例行HLA相合的同胞间异基因外周干细胞移植(Allo-PBSCT)、2例行自体外周干细胞移植(APBSCT)和1例行自体骨髓移植(Au-to-BMT)。结果:全部病例均成功造血重建。中性粒细胞≥0·5×109/L平均时间为11天;血小板≥20×109/L平均时间为16天;发生Ⅱ~Ⅲ度aGVHD1例,cGVHD1例,溶血性贫血2例,间质性肺炎2例,中位随访时间18(6~42)月,全部病例现均存活。结论:骨髓移植治疗恶性血液病有较好疗效。     

扶桑花抗生育成分对早孕小鼠黄体影响的定量研究

本文处用图像分析仪 ,以核浆比和数密度为指标 ,研究了不同浓度的扶桑花提取物—HR 1对早孕小鼠黄本组织学的影响。结果表明 :(1)小鼠黄体细胞的核浆比和数密度 ,随给药剂量 (0、 4、 10、 10 0、10 0 0mg/kg/d)的增加而增加 ,其中 10 0和 10 0 0mg/kg/d两组的黄体细胞核浆比和数密度明显高于对照组(p <0 .0 5) ;(2 )黄体组织学的定性观察显示 ,给药各组的黄体细胞明显退化 ,细胞缩小 ,细胞界限不清。这些结果提示 ,扶桑花提取物的抗早孕作用与妊娠黄体受损有关。