精神分裂症与神经症的反应时间比较

目的 探讨精神分裂症和精神症患者反应时间（RT）的特点。方法 应用美国CA－1000型电生理仪以及短音和视觉刺激,测查62例精神分裂症、54例神经症和67名正常对照组的反应时间。结果 精神分裂症组、神经症组及正常对照组的听反应时间和视反应时间两两比较均有非常显著差异（P＜0．01）。上述三组第二轮听反应时间与命令信号后负电位（PINV）变化表现为同一趋势,即听反应时间延迟,PINV随之延长。结论 反应时间可辅助精神分裂症患者和神经症患者的认知功能评定。     

Effects of arotinoid acid on induction of apoptosis and differentiation and telomerase activity and cell cycle in the HL～60 cell line

Objective To investigate the effects of arotinoid acid (Ro13-7410) on the morphological and functional alterations of leukemia HL-60 cell line and compared with those of RA.Methods Differentiation of HL-60 cells was assessed by morphology and by NBT reduction.Trypan blue exclusion was used to determine viability.Apoptosis was assessed by changes in cell morphology and by measurement of fragmented DNA using the PCD-assay-kit.Telomerase PCR ELISA-kit tested telomerase activity.The cell cycle was analyzed by flow cytometry.Results Incubation of the HL-60 cells with 10(-6)-10(-8) mol/L Ro13-7410 resulted in suppression of cell growth.Apoptotic cells were detected following exposure to 10(-6) mol/LRo13-7410 for 3 hours by measurement of the 'in situ' enzymatic labeling of DNA breaks with biotinylated dUTP.Ultrastructural examination of Ro13-7410-treated samples showed cells with chromatin compaction and cytoplasm condensation and the presence of 'apoptotic bodies'.Cells induced into apoptosis were accompanied by increase of intracellular free Ca[2+].Percentage of HL-60 cells reduced NBT following incubation with Ro13-7410 was lower than with all-trans retinoic acid (RA) (27% vas 85%).Telomerase PCR-ELISA assay showed that HL-60 cells cultured in the absence of inducing agents had significant telomerase activity.Telomerase activity declined gradually after 10(-6) mol/L Ro13-7410 treatment, and changes becoming evident at 1 day.The inhibition of telomerase activity at day 5 of treatment with Ro13-7410 was less effective than with RA.DNA flow cytofluorimetric analysis revealed that Ro13-7410 caused partial cell arrest in the G(2)/M phase after a 2-day treatment and the percentage of cells arrested in the G(2)/M phase increased after 4-days treatment.With RA-treated cells, a reduction in the percentage of cells in the G(2)/M phase was observed after 2-day of treatment.Conclusion Our study shows that Ro13-7410 suppresses HL-60 cells growth mainly via the induction of apoptosis and is less effective than RA in induction differentiation.Ro13-7410 dramatically inhibits telomerase activity during the course of induction and results in G(2)/M arrest within 2 days.These findings suggest that Ro13-7410 is worthy of further study for its effects on leukemic cells.     

直接经皮冠脉内支架植入术治疗急性心肌梗死

目的：评价直接冠脉内支架植入术治疗急性心肌梗死的临床疗效。方法：１７例急性心肌梗死患者于症状发作后２～１４小时施行冠脉内支架术。结果：所有患者冠脉再通均获成功。ＴＩＭＩ血流均达到３级。无残余狭窄,血管再通距症状发作时间平均５．７小时,所有患者胸痛均在术后２小时内明显缓解;除２例ＳＴ段持续抬高外,其余病例均在术后２小时ＳＴ段下降〉５０％;心肌酶峰明显提前。１例左主干病变于术后１周发生严重泵衰竭死亡;     

国产环丙沙星注射液临床疗效的观察

目的 :评价国产环丙沙星注射液治疗下呼吸道、泌尿系及伤寒等细菌性感染的临床疗效、细菌学疗效和安全性。方法 :采用随机对照开放试验的方法 ,对国产环丙沙星注射液的临床疗效和细菌清除率进行了观察。结果 :国产环丙沙星注射液临床疗效有效率 84.0 % ,痊愈率 56.0 % ,与对照组头孢氨噻肟疗效一致 ,无显著性差异 ( P>0 .0 5)。国产环丙沙星注射液细菌清除率 76.9% ,与头孢氨噻肟比较疗效接近 ( P>0 .0 5) ,对肺炎克雷伯杆菌、大肠杆菌、变形杆菌、枸橼酸杆菌、伤寒杆菌及不动杆菌具有良好的清除作用 ,对铜绿假单胞菌亦有满意的抗菌活性。不良反应轻微 ,用药安全。结论 :国产环丙沙星注射液为广谱、高效、安全的抗菌药物 ,对不能耐受口服制剂者可避免胃肠道的直接刺激作用 ,对重症不能口服者提供了很好的给药途径     

复方替硝唑含漱液的制备与含量测定

目的:制备复方替硝唑含漱液,建立其含量测定方法并进行稳定性研究.方法:采用最大吸收波长法测定替硝唑的含量,用双波长等吸收点法测定醋酸氯己定的含量,恒温加速试验法考察稳定性.结果:替硝唑回归方程为C＝－0.039 68＋27.491 A,r＝0.999 9,醋酸氯己定回归方程为C＝0.062 47＋50.454 8△A,r＝0.999 99,精密度和回收率良好,置65℃(96 h),70℃(72 h),75℃(48 h)恒温水浴中加热,含量无明显下降.结论:复方替硝唑含嗽液制备方法简单,含量测定方法简便且稳定性好.     

HPLC测定克感滴鼻液中盐酸麻黄碱的含量

目的：建立HPLC测定克感滴鼻液中盐酸麻黄碱的含量。方法：采用ODS柱，以0.01mol／L磷酸二氢钾溶液-乙腈(45：55)为流动相；检测波长为215nm；流速为1．0mL／min。结果：线性范围为40～96μg/mL，平均回收率为96.1％，RSD=1．2％n=5)。结论：本法简便、快速、准确，可用于本制剂质量控制。     

亚硝基谷胱甘肽对大鼠肝微粒体谷胱甘肽转移酶的激活及机制

目的　探索一氧化氮供体亚硝基谷胱甘肽(GSNO)能否在体外通过S 亚硝酰化机制激活大鼠肝微粒体谷胱甘肽转移酶 (mGST)。方法　微粒体粗提物与GSNO体外共孵育 ,测定mGST催化动力学改变 ,结合N 乙基马来酰亚胺 (NEM )再激活实验和二巯基苏醇 (DTT)逆转实验 ,以及酶蛋白游离巯基和酶S 亚硝酰化蛋白的改变 ,研究酶的激活机制。结果　GSNO在 0 .12 5～ 2mmol·L- 1浓度范围内呈浓度和时间 (3～ 15min)依赖性地激活mGST ,NEM对酶的再激活效应消失 ,DTT可以逆转上述激活作用 ,同时酶蛋白游离巯基浓度依赖性减少 ,而S 亚硝酰化蛋白浓度依赖性增多。结论　GSNO体外可激活大鼠肝mGST ,激活机制可能与mGST第 4 9位半胱氨酸 (Cys4 9)的巯基被亚硝酰化形成S 亚硝基硫醇结构有关。     

上海郊区老年痴呆症病人α-雌激素受体及芳香烃受体基因多态性

目的：探索上海地区农民人群中α-雌激素受体基因(ER-α)及芳香烃受体基因(Ahr)不同基因形态和老年痴呆症高发风险的可能联系．方法：分别以PCR—RFLP和AS—PCR方法分析一组老年痴呆症患者(n=52)ER-α基因及Ahr基因的多态位点，同一地区的健康人群(n=125)为对照．结果：Alzheimer症病人ER-α基因两个位点突变形态频率显著高于对照人群(Pvu Ⅱ位点：P=0．023，OR=2．94，95％CI 1．13—7．71；Xba I位点：P=0．046，0R=2．28，95％ CI 1．003—5．17)．Ahr G1721A位点基因型频率在病人组和对照组间无显著差异．结论：ER-α基因多态性可能与Alzheimer症易感性个体差异有关，本工作不支持Ahr基因多态性与老年痴呆症高发风险间的可能关联．     

消癖汤治疗高催乳素血症216例

将281例高催乳素血症(HPRL)患者随机分成两组，治疗组(216例)用中药消癖汤治疗，对照组(65例)用溴隐停治疗，观察血中泌乳素(PRL)和相关激素水平及临床症状体征变化情况。结果：治疗组、对照组总有效率分别为94．9％、95．4％，两组血清PRL均较治疗前显著降低，FSH、E2均较治疗前显著提高。说明中药消癖汤有溴隐停相同作用，但无不良反应。     

云木香化学成分研究

云木香Saussurea Lappa C．B．Clarke，又名广木香，青木香，自印度引种入我国，现广泛栽培于西南地区。曾用拉丁名Aucklandia lappa Decne．，为多年生高大菊科草本植物，以其根茎入药，具有解除平滑肌痉挛、降压和抗菌作用。临床常用以治疗胃部胀满、消化不良、呕吐、腹痛和腹泻等症，具