A case of testicular tunica albuginea cyst with psammoma body

Cystic testicular masses have been considered rare but due to advances in ultrasonographic technologies their incidence has risen. Many testicular cystic masses are benign but there is a chance of malignancy. Psammoma bodies are found in various malignancies that occur in the genital tract of women but rarely in men. We report a case of testicular tunica albuginea cyst with psammoma bodies.     

RAD16-Ⅱ的物理特性及其在神经干细胞假体构建中的应用

目的:观察RAD16-Ⅱ的物理特性以及神经干细胞在其上黏附和增殖的情况,探讨其构建的细胞假体在脊髓损伤中可能的应用。方法:将RAD16-Ⅱ的饱和水溶液同与之等体积的1.8%的氯化钠溶液混合后静置24h,通过肉眼、光镜和扫描电镜观察其表面结构,并对其吸水性和溶液pH值的动态变化进行评价;将经鉴定的神经干细胞与其共同培养,扫描电镜下观察细胞在材料上的黏附情况,同时应用免疫组化方法评价神经干细胞在材料上的增殖和分化情况。结果:RAD16-Ⅱ在1.8%的氯化钠溶液中塑型后,肉眼和光镜下形态多变,其形状取决于塑型的容器形状;扫描电镜下其为规则的网状,网孔直径为54.00±3.24"m,纤维直径为9.00±1.57"m,网孔底部相互沟通,神经干细胞可在其上迁移和增殖。结论:RAD16-Ⅱ具有良好的物理特性,神经干细胞可在与其构建的假体中迁移和良好地增殖、分化,在脊髓损伤的修复中可能具有潜在的应用前景。     

脑出血患者QTd及心肌酶谱的变化及意义

 目的探讨脑出血时心电图QT离散度(QTd)及血清心肌酶谱变化,客观评价脑出血病情及继发心脏损害的危险性。方法对54例脑出血患者及46例健康者的心电图QTd及血清谷草转氨酶(AST)、乳酸脱氢酶(LDH)、肌酸磷酸激酶(CK)等心肌酶谱进行检测,并作了对照分析。结果脑出血患者QTd明显增加,心肌酶谱各项检测指标显著异常,与对照组比较,P＜0.01或P＜0.05,并与脑出血量多少相关。QTd增加与心肌酶谱多项指标升高呈正相关。结论脑出血患者心电图QTd及心肌酶谱显著异常。对QTd及心肌酶谱检测有助于判断脑出血病情及心脏受累程度,有利于脑源性猝死的预防。     

Erythema Toxicum Neonatorum: An Immunohistochemical Analysis

Erythema toxicum neonatorum is a benign rash of unknown etiology, present to various degrees in most term newborns and characterized by an accumulation of eosinophils in dermal lesions. The recruitment of leukocytes to tissues implicates the involvement of adhesion molecules, cytokines, and chemokines. We therefore performed immunohistochemistry on punch biopsy specimens from cutaneous lesions of ten 1-day-old infants with erythema toxicum using specific monoclonal antibodies directed against a variety of adhesion molecules, cytokines, chemokines, and cell type-specific membrane markers. Biopsy specimens of noninflamed skin from four matched newborns and four adults served as controls. The immunohistologic features of erythema toxicum in all 10 infants included a strong staining of the adhesion molecule E-selectin in the vessel wall and the presence of numerous inflammatory cells that were identified as dendritic cells (CD1a, CD83, HLA-DR, CD40, and ICAM-1 positive), eosinophils (EG2 positive), neutrophils (CD15 positive), macrophages (CD14, CD68, and Mac387 positive), and E-selectin-expressing cells. Furthermore, the lesions showed a high incidence of the proinflammatory cytokines interleukin (IL)-1alpha and IL-1beta and of the chemokines IL-8 and eotaxin. This immunologic activity was reduced or absent in noninflamed skin from newborn controls and adults. We conclude that there is an accumulation and activation of immune cells in the lesions of erythema toxicum, also present in noninflamed skin of 1-day-old infants, but to a lower level. The physiologic significance of the rash remains to be elucidated.     

一株粪产碱杆菌(Alcaligenes faecalis)产热凝胶的发酵条件

通过对一株粪产碱杆菌WX-C12(Alcaligenes     

50例正常生育力男子精液和血液激素的一年动态观察

作者对南京市50名正常男子,每月1次、连续12个月测定了精液常规、某些精液生化和血清激素水平。结果表明:精子密度每次之间波动范围较大,冬季数值比夏季显著为高(P<0.01)。其它指标包括正常形态精子、活动精子、前向运动级别、精液果糖、硷性磷酸酶、酸性磷酸酶及血清,FSH LH T 则变动不显著。精液常规中除精子密度和各参数关系不明显外,所有其它参数之间呈正相关;但精液常规、精液生化和血清激素三者互不相关。从本组研究结果,作者提出了南京市正常男子这些指标的参考正常值。     

Inhibitors in congenital coagulation disorders

The development of inhibitory 'allo' antibodies to a deficient coagulation factor is arguably now the most severe and important complication of clotting factor concentrate exposure in haemophilia and other congenital coagulation disorders. Furthermore, development of an inhibitor to the factor VIII or factor IX transgene product remains a significant concern in gene therapy protocols for haemophilia. Although the development of an inhibitor does not usually change the rate, initial severity or pattern of bleeding, it does compromise the ability to manage haemorrhage in affected individuals, resulting in a greater rate of complications, cost and disability. The purpose of this review is to summarize current understanding of the epidemiology, immunobiology, laboratory evaluation and management of inhibitors arising in patients with congenital coagulation disorders. An attempt has been made to focus on recent advances in the immunology of inhibitors, and to speculate on their potential clinical application.     

缬沙坦和卡托普利干预高血压大鼠左室心肌纤维化及超微结构改变的比较研究

目的：比较缬沙坦和卡托普利对心肌纤维化及超微结构改变的干预作用。方法：14周龄自发性高血压大鼠(SHR)25只随机分为5组：缬沙坦组(SHRv)、卡托普利组(SHRc)、卡托普利组 缬沙坦组(SHRc v)、假治对照组(SHR20)和高血压基础对照组(SHR14)。同周龄SD大鼠5只设为正常对照组(SD20)。疗程为6周。处死后测算左室重量／体重比(LVM／BW)、光镜观察心肌胶原分布，测算胶原容积分数(CVF)、血管周围胶原面积／管腔面积比(PVCA／LA)和组织匀浆胶原浓度，电镜观察心肌超微结构改变。结果：三个治疗组反映心肌纤维化的多项指标均较SHR20和SHR14组显著改善(P＜0．0l＆0．05)。SHRv与SHRc v组的改善相似，均优于SHRc组(P＜0．05)，但仍差于SD20组(P＜0．05)。心肌超微结构改变均较SHR20组显著好转。结论：缬沙坦和卡托普利均能抑制高血压心肌纤维化及超微结构改变，但短期治疗不能恢复正常。缬沙坦的作用优于卡托普利，两者联用优于单用卡托普利，但并不显著优于单用缬沙坦。     

Monophasic action potentials at discontinuation of cardiopulmonary bypass: evidence for contraction-excitation feedback in man

Mechanical dysfunction is the strongest predictor of sudden cardiac death due to arrhythmia. Contraction-excitation feedback whereby changes in myocardial length/tension influence the time course of repolarization and excitability would provide a possible mechanism. Such a relationship has been shown in animals but has yet to be demonstrated in man. A useful model for studying this relationship is provided by the process of weaning off cardiopulmonary bypass after routine coronary artery surgery. During this weaning period of approximately 1 min, the heart is converted from being partially empty and flaccid (i.e., a "nonworking" state) to being filled and stretched to support the circulation (i.e., a "working" state). Monophasic action potentials (MAPs) were recorded from the left ventricular epicardium as a measure of repolarization time in 16 patients at discontinuation of cardiopulmonary bypass. Systolic pressure was recorded from the radial artery line. Measurements were made at three stages that related to different dynamic states of the heart: (1) starting to come off bypass ("minimally working"), defined as the time of first appearance of an inflection on the arterial pressure trace indicating the start of left ventricular ejection and valve opening, when arterial pressures represent left ventricular pressure, (2) half off bypass ("partially working"), and (3) off bypass ("wholly working"). During the process of discontinuing bypass MAP duration shortened, while systolic pressure increased. MAP duration at 90% and 60% repolarization (MAP D90, MAP D60) decreased from 288.0 +/- 29.5 msec (mean +/- SEM) and 235.0 +/- 27.9 msec in the minimally working heart to 274.5 +/- 30.2 msec and 224.2 +/- 27.3 msec in the partially working heart (p less than .001), with a subsequent decrease to 261.0 +/- 28.8 and 214.0 +/- 28.7 when the heart was wholly working (p less than .001). Systolic pressure increased from 54.1 +/- 9.3 mm Hg in the minimally working heart to 65.9 +/- 13.8 mm Hg in the partially working heart (p less than .001) and subsequently increased to 75.5 +/- 13.3 mm Hg when the heart was wholly working (p less than .001). Mean heart rates did not change significantly. A strong correlation was obtained between absolute MAP duration and systolic pressure. Regression analysis revealed: MAP D90 vs systolic pressure (p less than .001) and MAP D60 vs systolic pressure (p less than .01).(ABSTRACT TRUNCATED AT 400 WORDS)