全文获取类型
收费全文 | 1287篇 |
免费 | 93篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 37篇 |
妇产科学 | 67篇 |
基础医学 | 197篇 |
口腔科学 | 4篇 |
临床医学 | 168篇 |
内科学 | 242篇 |
皮肤病学 | 6篇 |
神经病学 | 160篇 |
特种医学 | 38篇 |
外科学 | 130篇 |
综合类 | 4篇 |
一般理论 | 3篇 |
预防医学 | 123篇 |
眼科学 | 4篇 |
药学 | 106篇 |
肿瘤学 | 88篇 |
出版年
2023年 | 5篇 |
2021年 | 20篇 |
2020年 | 11篇 |
2019年 | 18篇 |
2018年 | 32篇 |
2017年 | 21篇 |
2016年 | 16篇 |
2015年 | 38篇 |
2014年 | 48篇 |
2013年 | 70篇 |
2012年 | 73篇 |
2011年 | 83篇 |
2010年 | 51篇 |
2009年 | 52篇 |
2008年 | 67篇 |
2007年 | 62篇 |
2006年 | 65篇 |
2005年 | 69篇 |
2004年 | 51篇 |
2003年 | 49篇 |
2002年 | 44篇 |
2001年 | 57篇 |
2000年 | 51篇 |
1999年 | 46篇 |
1998年 | 10篇 |
1997年 | 7篇 |
1996年 | 12篇 |
1995年 | 6篇 |
1994年 | 5篇 |
1993年 | 13篇 |
1992年 | 15篇 |
1991年 | 18篇 |
1990年 | 20篇 |
1989年 | 18篇 |
1988年 | 16篇 |
1987年 | 11篇 |
1986年 | 19篇 |
1985年 | 16篇 |
1984年 | 27篇 |
1983年 | 11篇 |
1982年 | 8篇 |
1981年 | 10篇 |
1979年 | 6篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1971年 | 3篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1967年 | 3篇 |
1926年 | 2篇 |
排序方式: 共有1381条查询结果,搜索用时 15 毫秒
31.
B. Kaboré A. Post P. Lompo J.D. Bognini S. Diallo B.T.D. Kam J. Rahamat-Langendoen H.F.L. Wertheim F. van Opzeeland J.D. Langereis M.I. de Jonge H. Tinto J. Jacobs A.J. van der Ven Q. de Mast 《Clinical microbiology and infection》2021,27(4):590-596
ObjectivesAreas with declining malaria transmission in sub-Saharan Africa have recently witnessed important changes in the aetiology of childhood acute febrile illness (AFI). We describe the aetiology of AFI in a high malaria transmission area in rural Burkina Faso.MethodsIn a prospective hospital-based diagnostic study, children aged 3 months to 15 years with AFI were recruited and assessed using a systematic diagnostic protocol, including blood cultures, whole blood PCR on a selection of bacterial pathogens, malaria diagnostics and a multiplex PCR on nasopharyngeal swabs targeting 21 viral and 4 bacterial respiratory pathogens.ResultsA total of 589 children with AFI were enrolled from whom an infectious disease was considered in 575 cases. Acute respiratory tract infections, malaria and invasive bacterial infections (IBI) accounted for 179 (31.1%), 175 (30.4%) and 75 (13%) of AFI cases respectively; 16 (21.3%) of IBI cases also had malarial parasitaemia. A viral pathogen was demonstrated from the nasopharynx in 157 children (90.7%) with respiratory tract symptoms. Of all children with viral respiratory tract infections, 154 (92.4% received antibiotics, whereas no antibiotic was provided in 13 (17%) of IBI cases.ConclusionsViral respiratory infections are a common cause of childhood AFI in high malaria transmission areas, next to malaria and IBI. These findings highlight the importance of interventions to improve targeted treatment with antimicrobials. Most patients with viral infections received antibiotics unnecessarily, while a considerable number with IBI did not receive antibiotics. 相似文献
32.
33.
34.
35.
36.
37.
38.
Finny S. Varghese Esther van Woudenbergh Gijs J. Overheul Marc J. Eleveld Lisa Kurver Niels van Heerbeek Arjan van Laarhoven Pascal Miesen Gerco den Hartog Marien I. de Jonge Ronald P. van Rij 《Viruses》2021,13(2)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged as a new human pathogen in late 2019 and it has infected over 100 million people in less than a year. There is a clear need for effective antiviral drugs to complement current preventive measures, including vaccines. In this study, we demonstrate that berberine and obatoclax, two broad-spectrum antiviral compounds, are effective against multiple isolates of SARS-CoV-2. Berberine, a plant-derived alkaloid, inhibited SARS-CoV-2 at low micromolar concentrations and obatoclax, which was originally developed as an anti-apoptotic protein antagonist, was effective at sub-micromolar concentrations. Time-of-addition studies indicated that berberine acts on the late stage of the viral life cycle. In agreement, berberine mildly affected viral RNA synthesis, but it strongly reduced infectious viral titers, leading to an increase in the particle-to-pfu ratio. In contrast, obatoclax acted at the early stage of the infection, which is in line with its activity to neutralize the acidic environment in endosomes. We assessed infection of primary human nasal epithelial cells that were cultured on an air-liquid interface and found that SARS-CoV-2 infection induced and repressed expression of specific sets of cytokines and chemokines. Moreover, both obatoclax and berberine inhibited SARS-CoV-2 replication in these primary target cells. We propose berberine and obatoclax as potential antiviral drugs against SARS-CoV-2 that could be considered for further efficacy testing. 相似文献
39.
Signaling cascades initiated by nitric oxide (NO) and natriuretic peptides (NPs) play an important role in the maintenance of cardiovascular homeostasis. It is currently accepted that many effects of these endogenous signaling molecules are mediated via stimulation of guanylyl cyclases and intracellular production of the second messenger cGMP. Indeed, cGMP-elevating drugs like glyceryl trinitrate have been used for more than 100 years to treat cardiovascular diseases. However, the molecular mechanisms of NO/NP signaling downstream of cGMP are not completely understood. Recent in vitro and in vivo evidence identifies cGMP-dependent protein kinases (cGKs) as major mediators of cGMP signaling in the cardiovascular system. In particular, the analysis of conventional and conditional knockout mice indicates that cGKs are critically involved in regulating vascular remodeling and thrombosis. Thus, cGKs may represent novel drug targets for the treatment of human cardiovascular disorders. 相似文献
40.
SELF‐ATTRIBUTED SEASONALITY OF MOOD AND BEHAVIOR: A REPORT FROM THE NETHERLANDS STUDY OF DEPRESSION AND ANXIETY 下载免费PDF全文