赤芍注射液治疗慢性肺心病、肺动脉高压的临床研究

本研究用心肺阻抗血流图、血液粘度检查,动脉血气分析以及右心导管术等方法比较观察了42例肺心病患者静脉、肺动脉注射100%赤芍液的临床疗效。结果显示:赤芍注射液能扩张肺血管、降低肺动脉压力和肺血管阻力,增加心输出量,改善右心功能和血液流变性,提高氧分压,使临床瘀血体征显著改善,是防治肺心病、肺动脉高压较为适用的中药制剂。     

针灸治疗糖尿病50年重大进展概述

将我国近50年来针灸对糖尿病的临床治疗和实验研究的重大进展进行了综述。分别从方法学、治疗学的若干原则以及对糖代谢、胰腺内分泌激素、胰岛素抵抗、神经内分泌免疫网络、主要并发症的作用影响作了系统评价,对新世纪本病治疗的展望提出了建设性意见。     

红花黄色素对急性血瘀大鼠微循环及血液流变学部分指标的影响

目的观察红花黄色素(saffioryellow,SY)对血瘀大鼠微循环和血液流变性的影响。方法通过皮下注射肾上腺素注射液0.07ml/(kg     

Dissociative adsorption modes of TATB on the Al (111) surface: a DFT investigation

Herein, the adsorption modes and electronic structures of TATB/Al (111) systems were investigated using the density functional theory (DFT) approach. We found that chemical adsorption led to the decomposition of the TATB molecule on the Al surface by four adsorption modes. All the adsorption configurations were accompanied by fractures of the N–O bonds in the nitro groups. In addition, there was a hydrogen atom transfer for 5P. For parallel and vertical adsorptions, the TATB molecules favored planar or quasi-planar structures. The order of total energy with BSSE correction matches well with the order of adsorption energy. The absolute values of energy and adsorption energy of 6P and 6V are highest in the parallel and vertical adsorption systems, respectively. Electrons are transferred from the Al (111) surface to the TATB molecule; this results in the activation of TATB on the Al (111) surface and obvious augmentation of the PDOS (partial density of states) peaks of the N and O atoms. From the Al (111) surface to the TATB molecule, the transfer of the electrons of 4P (14.00e) and 6V (9.04e) is largest for the parallel and vertical adsorptions, respectively.

The four adsorption modes are accompanied by fractures of the N–O bonds and transfer of a hydrogen atom.     

热休克蛋白27对人晶状体上皮细胞氧化损伤的保护作用及其机制

目的:观察热休克预处理诱导的热休克蛋白27对晶状体上皮细胞的保护作用。方法:实验于2004-01/12在中国医科大学卫生部小儿先天畸形重点实验室完成。体外培养人晶状体上皮细胞系HLB-C3细胞,随机分为4组:①正常对照组。②氧化损伤组:细胞中加入200μmol/LH2O2作用6h。③热休克处理组:42℃预热30min后,37℃恢复2h,处理同氧化损伤组。④放线菌酮组:预热前30min加入放线菌酮(25mg/L),其余处理同热休克处理组。观察各组细胞存活率、超氧化物歧化酶和过氧化氢酶活性;DNAladder和AnnexinⅤ-FITC流式细胞仪检测晶状体上皮细胞凋亡率;免疫印迹法检测各组中热休克蛋白27和半胱氨酸天冬氨酸蛋白酶3的表达。结果:①细胞活力:氧化损伤组低于正常对照组(0.24±0.02,0.28±0.04,P<0.01),热休克处理组高于氧化损伤组(0.27±0.04,P<0.05),放线菌酮组低于热休克处理组(0.23±0.01,P<0.01)。②细胞凋亡率:氧化损伤组高于正常对照组[(15.69±1.54)%,(4.17±0.83)%,P<0.01];热休克处理组低于氧化损伤组[(8.34±1.19)%,P<0.01];放线菌酮组高于热休克处理组[(13.58±1.21)%,P<0.01]。③超氧化物岐化酶和过氧化氢酶活性:氧化损伤组低于正常对照组(P<0.01),热休克处理组高于氧化损伤组(P<0.05),放线菌酮组低于热休克处理组(P<0.01)。④热休克蛋白27表达:正常对照组细胞中略有表达,氧化损伤组表达稍有增加,热休克处理组表达明显增多,放线菌酮组未见表达。⑤半胱氨酸天冬氨酸蛋白酶3的表达:正常对照组细胞表达较弱,氧化损伤组表达增多,热休克处理组的表达少于氧化损伤组。结论:热休克预处理诱导的热休克蛋白27对氧化损伤的晶状体上皮细胞起着保护作用。其保护机制可能是:①对与晶状体抗氧化有关的酶防御系统的影响:②小分子热休克蛋白对抗细胞凋亡。     

内毒素诱导性基因启动子结合蛋白的筛选新方法及其应用

目的建立研究DNA-蛋白质相互作用的新方法。方法选择6只SPF级BALB／c小鼠,模型组经尾静脉注射脂多糖（LPS）25mg／kg,使平均动脉压（MAP）降至40mmHg（1mmHg=0．133kPa）造成内毒素休克,然后迅速处死,正常对照组同期处死,取肝脏组织。利用生物素一链亲和素系统结合磁珠分离技术筛选内毒素诱导基因（LIG）启动子的结合蛋白。用聚合酶链反应（PCR）扩增末端带生物素标记的LIG启动子探针,提取动物肝脏组织胞核蛋白,与制备的LIG启动子探针进行孵育,再以标记有链亲和素的磁珠分离LIG启动子-蛋白反应复合物,使用不同浓度NaCl洗脱结合的蛋白,使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS—PAGE）分离样品蛋白,并对凝胶进行银染显色,比较模型组与正常对照组的差异条带。结果两组胞核蛋白中共观察到15条差异条带。分析差异条带显示,与正常对照组比较,在低亲和力作用水平,LPS作用后有4个蛋白开始与LIG启动子作用力增强,而有1个蛋白则同启动子的相互作用减弱;在DNA-蛋白质高亲和力作用水平,则有9个蛋白因LPS刺激而同DNA的结合力增强,有1个蛋白同启动子的相互作用消失。结论成功建立了生物素-链亲和素结合磁珠分离技术的新方法,为研究DNA-蛋白质相互作用开拓了新思路,并从“转录调控组学”角度深入理解基因表达调控机制奠定了基础。     

军队医院聘用护士科研能力的调查分析

目的　了解聘用护士对科研活动的认识及科研水平现状。方法　自行设计问卷 ,调查 10 5名聘用护士的科研能力。结果　聘用护士对参加科研活动、撰写论文的认识不足 ,工作 2～ 6年的聘用护士撰写与发表论文较少 ,发表率与军人护士比较 ,经统计学分析 ,有显著性差异 (P <0 .0 1)。结论　重视聘用护士科研能力的培养 ;对聘用护士参加科研活动 ,做出明确要求 ;定期举办论文写作学习班及新业务、新知识讲座 ;提高专业及外语理论水平 ;加大护理信息量等举措是提高聘用护士科研素质的有效途径。     

Early manifestation of alteration in cardiac function in dystrophin deficient mdx mouse using 3D CMR tagging

Background

Duchenne muscular dystrophy (DMD) is caused by the absence of the cytoskeletal protein, dystrophin. In DMD patients, dilated cardiomyopathy leading to heart failure may occur during adolescence. However, early cardiac dysfunction is frequently undetected due to physical inactivity and generalized debilitation. The objective of this study is to determine the time course of cardiac functional alterations in mdx mouse, a mouse model of DMD, by evaluating regional ventricular function with CMR tagging.

Methods

In vivo myocardial function was evaluated by 3D CMR tagging in mdx mice at early (2 months), middle (7 months) and late (10 months) stages of disease development. Global cardiac function, regional myocardial wall strains, and ventricular torsion were quantified. Myocardial lesions were assessed with Masson''s trichrome staining.

Results

Global contractile indexes were similar between mdx and C57BL/6 mice in each age group. Histology analysis showed that young mdx mice were free of myocardial lesions. Interstitial fibrosis was present in 7 month mdx mice, with further development into patches or transmural lesions at 10 months of age. As a result, 10 month mdx mice showed significantly reduced regional strain and torsion. However, young mdx mice showed an unexpected increase in regional strain and torsion, while 7 month mdx mice displayed similar regional ventricular function as the controls.

Conclusion

Despite normal global ventricular function, CMR tagging detected a biphasic change in myocardial wall strain and torsion, with an initial increase at young age followed by progressive decrease at older ages. These results suggest that CMR tagging can provide more sensitive measures of functional alterations than global functional indexes in dystrophin-related cardiomyopathies.     

Castleman病临床及病理类型与合并症关系分析:单中心大宗病例观察

目的 研究Cagtleman病(CD)的临床及病理类型与各种合并症的关系,推测各种合并症的发病机制,以提高对本病的认识.方法 回顾性分析北京大学第一医院自1977年以来共53例CD患者的临床特点,同时分析临床合并症与临床类型和病理类犁的关系.结果 53例CD患者中单中心型32例(60.4%),多中心型21例(39.6%);病理分型示透明血管型(HV)37例(69.8%),浆细胞型(PC)9例(17.0%),混合型(MIX)7例(13.2%).患者中32例有合并症,占60.4%.根据受累器官不同分为累及皮肤、内脏和血液系统.临床类型与某些合并症的发生密切相关,32例单中心型的主要合并症为副肿瘤性天疱疮(PNP)和闭塞性细支气管炎(BO),21例多中心型的主要合并症为肾脏、血液改变.病理类型与合并症的发生密切相关:透明血管型主要合并症为PNP和BO,浆细胞型和混合细胞型主要合并症为内脏和血液受累.合并PNP的CD患者的临床和病理分型均不同于其他CD.Kaplan-Meier分析显示:伴有合并症的患者生存率明显低于无合并症的患者(P=0.028).结论 CD患者的合并症与其临床、病理分型有关,合并PNP的CD患者应单独分型,有无合并症是影响CD患者预后的关键因素.