YB-1在子宫内膜异位症中的表达

目的探讨YB-1在子宫内膜异位症(EMs)患者血清及子宫内膜组织中的表达及意义,以期将YB-1作为EMs早期诊断和治疗的判定指标。方法采用ELISA法检测EMs组与对照组血清中YB-1的表达水平,采用免疫组化SP法检测两组子宫内膜组织中YB-1的表达情况。结果与对照组(9.64±2.37 ug/L)相比,EMs组患者血清YB-1的平均浓度(14.65±3.91 ug/L)较高,二者相比差异有统计学意义(p<0.05)。免疫组化结果显示YB-1在EMs组患者子宫内膜中主要呈中等强度阳性表达。正常子宫内膜中也可见YB-1的少量弱阳性表达,两组子宫内膜组织中YB-1的阳性表达率相比有明显差异(p<0.05)。结论 YB-1在EMs患者血清及子宫内膜组织中高表达,其不仅可作为EMs细胞内的标志物,也有望成为一个理想的血清学标志物。     

锌指蛋白KLF12抑制人子宫内膜间质细胞体外蜕膜化过程中胰岛素样生长因子结合蛋白-1表达

目的人子宫内膜间质细胞蜕膜化标志蛋白胰岛素样生长因子结合蛋白-1(insulin-like growth factor binding protein-1,IGFBP-1)参与调控月经周期、排卵、蜕膜化、胚胎种植以及胎儿生长等生理过程,本研究旨在探索锌指蛋白KLF12对人子宫内膜间质细胞体外蜕膜化过程中IGFBP-1表达的影响。方法制备Ad-Flag-KLF12重组腺病毒,通过荧光定量聚合酶链式反应(PCR)和酶联免疫吸附试验(ELISA)结合重组腺病毒介导的KLF12过表达实验,阐述人子宫内膜间质细胞体外蜕膜化过程中KLF12对IGFBP-1表达调控作用。结果成功获得滴度为2×1011ifu/ml的重组KLF12腺病毒,该腺病毒可在人子宫内膜间质细胞中高表达Flag-KLF12融合蛋白;体外分离培养的人子宫内膜间质细胞经8-Br-cAMP和醋酸甲羟孕酮(medroxy progesterone acetate,MPA)联合诱导蜕膜化后,KLF12的mRNA表达随着诱导时间延长逐渐降低,72h降低最明显,较未刺激降低约70%;过表达KLF12显著抑制8-Br-cAMP和MPA诱导的人子宫内膜间质细胞中IGFBP-1mRNA的表达,并以时间依赖的方式明显抑制IGFBP-1的分泌(P0.01);过表达KLF12明显抑制人子宫内膜间质细胞体外蜕膜化过程中IGFBP-1启动子转录活性。结论锌指蛋白KLF12抑制人子宫内膜间质细胞体外蜕膜化过程中IGFBP-1的表达与分泌。     

辅酶Q10对大鼠实验性牙周炎的影响

目的探讨辅酶Q10对大鼠实验性牙周炎的影响。方法选取体重200g左右健康Wistar大鼠48只。大鼠随机分为3组:空白对照组(N组)、牙周炎辅酶Q10治疗组(Q10组)及牙周炎对照组(P组),每组16只。N组正常食水;另外两组建立牙周病模型。成模后,Q10组大鼠连续给药12周,P组给同剂量生理盐水,每组动物分别在给药前及给药第4周、8周、12周末行双侧下颌第一磨牙牙周袋平均深度的检测,处死后测量双侧下颌第一磨牙牙槽骨垂直骨吸收总量,并行统计学分析。结果给药前及给药后各观察时间点Q10组及P组大鼠牙周袋平均深度及牙槽骨垂直骨吸收总量均显著大于N组(P<0.01);且给药前Q10组及P组大鼠牙周袋平均深度及牙槽骨垂直骨吸收总量均无统计学差异(P>0.05);12周末P组的牙周袋平均深度及牙槽骨垂直吸收总量均明显大于Q10组(P<0.01)。结论辅酶Q10可以抑制和缓解实验性大鼠模型牙周炎,可能具有治疗和缓解牙周炎症的作用。     

Combination of Aβ clearance and neurotrophic factors as a potential treatment for Alzheimer’s disease

There is no effective drug to treat Alzheimer’s disease (AD), a neurodegenerative disease affecting an estimated 30 million people around the world. Strongly supported by preclinical and clinical studies, amyloid-beta (Aβ) may be a target for developing drugs against AD. Meanwhile, the fact that localized neuronal death/loss and synaptic impairment occur in AD should also be considered. Neuronal regeneration, which does not occur normally in the mammalian central nervous system, can be promoted by neurotrophic factors (NTFs). Evidence from clinical trials has shown that both Aβ clearance and NTFs are potentially effective in treating AD, thus a new approach combining Aβ clearance and administration of NTFs may be an effective therapeutic strategy.     

我国人群细胞因子水平与抑郁症的相关性研究的系统评价

目的:系统评价我国人群细胞因子水平与抑郁症的相关性。方法:计算机检索PubMed、中国生物医学文献数据库、维普中文数据库、中国期刊全文数据库和万方数据库,收集研究国内细胞因子与抑郁症相关性的病例对照研究,由两名研究者按照纳入与排除标准筛选文献、提取资料和评价质量后,采用Rev Man 5.1.5统计软件进行Meta分析。结果:共纳入7项研究,合计577例患者,其中试验组331例,健康对照组246例。Meta分析结果显示,试验组患者白细胞介素(IL)-6浓度[WMD=13.71,95%CI(6.91,20.51),P<0.000]和IL-1β浓度[SMD=2.15,95%CI(1.04,3.26),P<0.000]均高于健康对照组,两组比较差异有统计学意义;而抑郁组患者IL-2浓度亦高于健康对照组,但两组比较差异无统计学意义。结论:我国人群患抑郁症与IL-6、IL-1β水平升高存在一定的相关性。由于本研究的局限性,IL-6、IL-1β浓度与抑郁症的发作性质、严重程度的定量关系等内容尚需开展更多高质量、大样本的研究。     

Orthogonal Design on Range Hood with Air Curtain and Its Effects on Kitchen Environment

Conventional range hoods cannot effectively prevent the oil fumes containing cooking-induced harmful material from escaping into the kitchen Air curtains and guide plates have been used in range hoods to reduce the escape of airborne emissions and heat, thereby improving the kitchen environment and the cook's degree of comfort. In this article, numerical simulations are used to study the effects of the jet velocity of an air curtain, the jet angle of the air curtain, the width of the jet slot, the area of the guide plate, and the exhaust rate of the range hood on the perceived temperature, the perceived concentration of oil fumes, the release temperature of oil fumes, and the concentration of escaped oil fumes in a kitchen. The orthogonal experiment results show that the exhaust rate of the range hood is the main factor influencing the fumes concentration and the temperature distribution in the kitchen. For the range hood examined in the present study, the optimum values of the exhaust rate, the jet velocity of the air curtain, the jet angle of the air curtain, the width of the jet slot, and the area of the guide plate are 10.5 m³/min, 1.5 m/s, ?5°, 4 mm, and 0.22 m², respectively, based on the results of the parametric study. In addition, the velocity field, temperature field, and oil fumes concentration field in the kitchen using the proposed range hood with the air curtain and guide plate are analyzed for those parameters. The study's results provide significant information needed for improving the kitchen environment.