Synthesis and characterization of amphiphilic and microphase-separated graft copolymers, 1. Synthesis and bulk characterization of polystyrene-graft-ω-stearyl-poly(ethylene oxide)

Using the rigid and hydrophobic polystyrene (PS) chain as backbone, onto which flexible and hydrophilic stearyl-poly(ethylene oxide) (SPEO) chains are grafted, a new kind of amphiphilic, microphase-separated graft copolymer was synthesized using the macromonomer technique. Stearyl-poly(ethylene oxide) macromonomers with acryloyl end-group (SPEO-A) were prepared through an end-group exchange reaction of α-stearyl-ω-hydroxypoly(ethylene oxide) (SPEO-OH) and acryloyl chloride in the presence of triethylamine. The radical copolymerization of styrene with SPEO-A was carried out under various experimental conditions. Following a careful examination of their purity, the structure of the prepared copolymers was characterized by means of IR, ¹H NMR and GPC analyses. A new feasible method using first derivative UV spectrometry was developed for quantitative determination of the bulk composition of the graft copolymers. Copolymers with a wide range of bulk composition and satisfactory grafting degree were obtained.     

女贞子、刺五加对T细胞促进作用的实验研究

女贞子和刺五加都是扶正中药.女贞子在体内体外对 T 细胞均有促进作用,但对其作用机理报导很少。刺五加是典型的“适应原样”药物,对机体免疫功能亦有促进作用,但其对 T 细胞的作用尚未见报导.     

抗阴道毛滴虫单克隆抗体的制备及其特性

用杂交瘤技术建立了6株能稳定分泌抗阴道毛滴虫单克隆抗体的细胞株,选取其中3株进行了分析。3株单抗的效价分别是:ⅣA12B1和ⅣA22B1为250000ⅡB2A1为1250000。ⅣA12B1和ⅡB2A为IgG3抗体,ⅣA2281为IgGl抗体。位点分析及免疫酶染色发现,ⅣA12B1和ⅣA22B1抗同一抗原决定簇,所针对的抗原主要分布于膜上;ⅡB2A1针对的抗原主要分布于胞浆中。3株单抗针对的抗原均为糖蛋白。特异性试验发现,这3株单抗与人白细胞、阴道上皮细胞、白色念珠菌、大肠杆菌及人毛滴虫均无交叉反应。用制备的单克隆抗体,建立了试断滴虫性阴道炎的胶乳凝集试验(LAT)。研究表明,LAT是一种敏感、特异、简单和快速的方法。     

Long-term survival after autologous bone marrow transplantation for follicular lymphoma in first remission.

The role of autologous stem cell transplantation (ASCT) in the treatment of follicular lymphoma is still being defined in the era of antibody therapy. Here we report the long-term 12-year clinical outcomes of patients treated with autologous bone marrow transplantation (ABMT) for follicular non-Hodgkin's lymphoma (NHL) in first remission. Between 1988 and 1993, advanced-stage follicular NHL patients in need of initial therapy were enrolled in 2 consecutive prospective treatment trials of either standard-dose CHOP induction (83 patients) or high-dose CHOP plus granulocyte-colony stimulating factor (G-CSF) (20 patients). Patients who achieved an adequate remission with induction therapy underwent conditioning with cyclophosphamide and total body irradiation (TBI) followed by ABMT in first remission using bone marrow (BM) purged in vitro with anti-B cell monoclonal antibodies and rabbit complement (96 patients). At 12-year follow-up, 61% of the patients are alive and 43% remain in continuing complete remission. The only predictors of decreased progression-free survival proved to be histologic BM involvement at time of harvest (hazard ratio [HR] 2.27, 95% confidence interval [CI] 1.3-3.9, P<.004) and PCR detectable disease in the BM product after purging (HR 4.18, 95% CI 1.99-8.8, P=.0002). No significant predictors of overall survival were identified. These results at 12-year follow-up suggest that a subset of follicular lymphoma patients can experience prolonged survival with ABMT in first remission.     

THE ACTION OF RADIX REHMANNIAE AND PLASTRUM TESTUDINIS ON BETA- ADRENERGIC RECEPTOR-cAMP SYSTEM

Mouse and rat pathological models were pro duced by injections of thyroid hormone (T3 0r T4, Model. I) or hydrocortisone (HC, Model II). A single subcutaneous dose of isoproterenol (ISO) caused plasma cAMP to peak in 5-15 min which was significantly higher in mouse Models I and II than in normal mice. Daily oral administration of a decoction of two Yin tonics-Radix Rehmanniae (RR) and Plastrum Testudinis (PT) reduced peak cAMP in both models. 3H.DHA binding assay re vealed significant elevations of the number of 3 adrenergic receptors in rat Models I and II. Decoc- tion RR and PT restored the peaks to normal range. Yang tonics Radix Aconiti praeparata (RAP) and Cortex Cinnamomi (CC) showcd contradictory effects on these models. It seems unlikely that the action of the two tonics promotes the dlearance of injected hormones, since injected hormone RIA iii plasma showed no differences between Yin tonics treated and untreated models. The therapeutic effects of these two Yin tonics on "Yin deficiency" syndrome of hyperthyroidism or hyperfunction of the adrenal cortex is very probably related to their actions on the O-adrenoreceptor-cAMP system as stated.     

842例工人、农民和战士最大呼气流量-容积曲线的测定

作者用“点值法”对成都市内和郊区842例从事轻、中、重劳动的健康工人、农民和战士作了MEFV曲线和常规肺功能测定,发现工人和农民在低肺容积时流量增加,在高、中肺容积时流量下降,并以农民最明显,重劳动工人次之,轻、中劳动工人较不明显,战士则相反。揭示不同的体力活动方式及生活条件可导致不同肺容积时的流量改变,以适应其代谢增强时的耗氧需求。     

农业科技期刊论文发表中的信息安全问题及应对策略

【目的】 分析农业科技论文中的信息安全问题,指出信息安全关键点,为防止因学术传播泄露国家(企业)机密和保护信息安全提供参考。【方法】 依据我国相关法律法规以及编辑工作实践,对农业科技期刊论文发表中的信息安全关键点进行分析。【结果】 总结出农业科技论文中的信息安全关键点,即全国性的农业基础数据、涉密的科研项目、生物育种技术、食品安全、动物疫情、植物病害、地理信息、气象信息等。针对信息安全问题提出如下应对策略:提高编辑人员信息安全意识和涉密数据识别能力、建立完整的信息安全审查机制、联合探索信息权限控制技术手段、对作者加强信息安全知识宣传、增加行业期刊间的沟通与交流。【结论】 编辑部应将国家安全和利益放在重要位置,以国家法律法规以及农业农村部的有关规定为依据,把控农业科技论文信息安全关键点,确保发表论文的信息安全。