布洛芬缓释片的研究

目的：研究布洛芬缓释片的制备,减少给药次数,降低不良反应。方法：采用正交设计法制备缓释片,转篮法测定其释放度并考察其稳定性。结果：缓释片累积释放量(7h)大于75％,释放行为符合Higuehi方程,稳定性良好。结论：该缓释片处方合理,工艺简单,有理想的缓释效果。     

Effect of intravenous immunoglobulin on inhibiting peripheral blood lymphocyte apoptosis in acute Kawasaki disease

This study aimed to explore the therapeutic mechanism of intravenous immunoglobulin (IVIG) for Kawasaki disease (KD). Peripheral blood lymphocytes (PBLs) obtained from 26 children with KD and 20 age-matched healthy children were stimulated with anti-CD3 monoclonal antibody (mAb), and the percentage of apoptotic cells and DNA fragmentation were assayed at 0, 12, 24, 48 and 72 h in vitro. The patients were divided into two groups: one treated with aspirin combined with IVIG (n = 16) and one treated with aspirin alone (n = 10). PBLs were stimulated by phytohaemagglutinin to evaluate the lymphocyte proliferative response. Compared with normal controls, the apoptotic cell percentage and the DNA fragmentation were markedly decreased (p < 0.001) and delayed in PBLs from KD patients. After IVIG treatment, the decreased percentage of apoptotic cell and delayed DNA fragmentation were restored to the state of the normal controls, accompanied by a fast clinical remission compared with the aspirin-alone group. The lymphocyte proliferative response was also decreased 3-5 d after IVIG therapy (p < 0.001). Conclusion: The results suggest that decreased PBL apoptosis may be involved in the pathogenesis of KD. The therapeutic mechanism of IVIG in KD may be partially due to the reversal of the inhibited lymphocyte apoptosis, and may have implications for other autoimmune diseases with inefficient lymphocyte apoptosis.     

白头翁中毛莨甙及合成毛茛甙的高效液相色谱测定法

白头翁为毛莨科属植物Pulsatilla chinensis,以根入药,其煎剂能抑制阿米巴原虫的生长、杀灭阴道滴虫等作用,用于细菌性痢疾和阿米巴痢疾的治疗。白头翁中主要成分有原白头翁素[protoanemonin,系由毛莨甙(ranunculin)酶解而得]、白头翁素(anemonin)等,其结构如下:     

Different Effects of Estrogen and Progesterone on K+ Currents Expressed in Xenopus Oocytes

Estrogen has received considerable attention recently as a potential therapeutic agent in vascular pathophysiological states such as stroke. The mechanisms by which estrogen influences cerebral arteries are incompletely understood. The present study was to examine the effect of ovariectomy and chronic estrogen or tamoxifen treatment on vascular reactivity in rat posterior communicating cerebral arteries with intact endothelium. Changes in vascular tension were measured by microvessel myograph. Ovariectomy significantly enhanced the constricting responses to endothelin I, but not to phenylephrine. Chronic treatment with estrogen or tamoxifen partially reversed or abolished the effect of ovariectomy. The contraction induced by high K⁺ solution was also enhanced in the ovariectomized rats and this enhancement was abolished by estrogen or tamoxifen treatment. Ovariectomy potentiated the relaxant response to nicardipine but not NS 1619. Estrogen but not tamoxifen reversed the effect of ovariectomy. The present results indicate that chronic tamoxifen may not act as an antagonist of estrogen, instead, chronic treatment with estrogen and tamoxifen has similar effect in inhibiting the increased vascular tension induced by ovariectomy. This study suggests the influence of physiological level of estrogen on vascular contractility. It is at present unknown what may have caused increased relaxant effect of nicardipine, a L-type Ca²⁺ channel blocker. More experiments are needed to show the role of endothelium in the altered vascular contractility in the ovariectomized and estrogen-treated rats. (supported by UPGC Direct Grant).     

高效液相色谱法测定血浆中醋氯芬酸及其在犬体内药代动力学

建立了HPLC法同时测定家犬血浆中的醋氯芬酸及其主要代谢物的浓度。此法简便易行,精密度好,方法回收率91.3%～96.9%,日内、日间RSD为3.69%～8.13%,血药浓度在0.050～51.2μg·mL^-1范围内呈线性关系,相关系数0.9998,当S/N≥3时,最小检测浓度为10ng·mL^-1。此法可同时测定醋氯芬酸及其在体内的主要代谢物。醋氯芬酸po吸收迅速,给药后约12min即达血药浓度峰值,其药—时曲线符合二室模型,T_1/2α仅为2.5min左右,T_1/2β约为137min,代谢物约在110min达到血药浓度峰值,峰浓度为3.20μg·mL^-1,T_1/2β约为140min。     

胃复安引起过敏性荨麻疹1例

1　病例报告　男 ,2 4岁 ,因晨起饮冷开水后 ,阵发性上腹痛伴恶心 1h,呕吐 1次来就诊 ,其间无腹泻 .既往无眩晕及肝肾疾病史 .查体 :T36 .8℃ ,HR79次· min- 1 ,BP14.1/10 .2 k Pa,一般状况可 ,咽、扁桃体不充血 ,心肺未见异常 ,腹部平坦 ,未见明显肠型及蠕波 ,左上腹深压痛 ,无肌卫及反跳收稿日期 :2 0 0 0 -0 7-2 1;　修回日期 :2 0 0 0 -0 8-2 3作者简介 :丁向清 (196 8-) ,男 (汉族 ) ,湖北省应城市人 .主治医师 .Tel.(0 431) 79710 16 Ext. 6 6 5 12痛 ,肝脾未及 ,肠鸣音略亢进无气过水声 ,肾区无叩痛 ,胸腹透未见异常 ,WBC 5 .3…     

百日咳杆菌毒素S_1亚单位与谷胱甘肽S-转移酶融合基因在大肠杆菌中表达及其一步纯化

研究了百日咳杆菌毒素Ｓ１亚单位与谷胱甘肽Ｓ－转移酶融合蛋白（ＧＳＴ－Ｓ１）在大肠杆菌（Ｅ．ｃｏｌｉ）中的表达,并一步纯化了重组ＧＳＴ－Ｓ１。首先用聚合酶链反应（ＰＣＲ）扩增出长度为５６０ｂｐ的编码百日咳毒素Ｓ１亚单位的ＤＮＡ片段。该片段比原编码Ｓ１蛋白的ＤＮＡ片段在其３′端缺失了１３８个碱基,将其按正确的阅读框克隆入质粒ｐＧＥＸ中ＧＳＴ基因的３′端,构成ＧＳＴ－Ｓ１融合基因表达载体ｐＧＥＸ－Ｓ１。经异丙基硫代－β－Ｄ－半乳糖苷（ＩＰＴＧ）诱导表达和十二烷基磺酸钠－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）,可见所表达的ＧＳＴ－Ｓ１融合蛋白存在于细菌上清,为可溶性蛋白。分子量为４９ｋｕ处有明显的蛋白表达带。用谷胱甘肽（ＧＳＨ）亲和层析系统,一步纯化出ＧＳＴ－Ｓ１融合蛋白。最后经凝血酶的消化,得到２３ｋｕ的重组百日咳杆菌毒素Ｓ１亚单位蛋白。为百日咳疫苗的发展提供了依据     

关节镜辅助经皮螺丝钉内固定与切开复位记忆爪内固定治疗髌骨骨折：两种植入体材料物理性能与修复效果的关系

目的:观察膝关节镜辅助经皮螺丝钉内固定与切开复位髌骨爪内固定两种植入体材料特征及治疗髌骨骨折的疗效,探讨植入体选择与其效果的相关性。方法:①试验对象:选择2001-11/2005-12本院在膝关节镜下经皮复位螺丝钉内固定治疗髌骨骨折30例做为镜下组,选骨折类型相似的患者切开复位髌骨爪内固定治疗髌骨骨折30例做为髌骨爪组。所有患者术前签知情同意书及手术同意书。②试验方法:镜下组在关节镜下闭合复位,用两枚平行螺丝钉内固定;髌骨爪组切开复位,用记忆合金髌骨爪内固定。③试验评估:根据胥少汀等评分法评价膝关节功能并在术后进行随访观察。结果:①术后嘱患者按1,3,6,12个月门诊复诊,随访9～13个月,平均12个月。②两组患者术后随访优良率:镜下组优22例(73%),良7例(23%),可1例(3%);髌骨爪组优21例(70%),良7例(23%),可1例(3%),差1例(3%)。③镜下组与髌骨爪组平均出血量分别为(30±14),(100±50)mL;手术时间(60±15),(80±15)min,住院时间(8±2),(16±3)d,差异显著(P<0.01)。结论:两种方法术后关节功能恢复均良好,关节镜下经皮螺丝钉内固定治疗髌骨骨折创伤小、出血少、复位确切、有利于术后关节早活动。     

非创伤性股骨头坏死的舌象定量研究

目的:探讨定量采集分析舌象特征在非创伤性股骨头坏死中医辨证中的应用价值。方法:采用DS01-B舌面诊测信息采集仪采集分析273例非创伤性股骨头坏死患者的舌象信息,对所获得的舌象信息进行聚类分析,同时分析非创伤性股骨头坏死国际骨循环研究会(Association Research Circulation Osseous,ARCO)分期与舌象特征的关系。结果:273例患者共涉及18项舌象信息,舌色以淡紫、暗红、淡红为主;舌形以胖、齿痕、裂纹为主;苔色以白、灰黑、黄白相兼为主;苔质以薄苔、腻苔为主。经聚类分析,最终将273例非创伤性股骨头坏死患者的舌象分为3类。第1类,舌胖、苔薄白;第2类,舌暗红或淡紫、苔黄白相间;第3类,舌淡红,有齿痕、裂纹,苔厚、腻或少苔、无苔,颜色灰黑。ARCOⅠ期患者舌色为淡紫、舌形为胖、苔色为灰黑、苔质少;ARCOⅡ、Ⅲ期患者舌色以暗红、淡紫、淡红为主,舌形以胖为主,苔色以白为主,苔质以薄苔为主;ARCOⅣ期患者舌色以暗红为主,舌形以胖、齿痕为主,苔色以黄白相兼、灰黑为主,苔质以薄苔为主。结论:通过舌面诊测信息采集仪定量采集分析的舌象信息可准确反映非创伤性股骨头坏死患者的舌象特征,可作为非创伤性股骨头坏死的中医辨证依据。