单唾液酸神经节苷脂对体外循环后大鼠脑组织一氧化氮合成酶活性的影响

目的:研究体外循环(CPB)后脑组织的一氧化氮合成酶(NOS)活性的变化及单唾液酸神经节苷脂(GM1)干预后对其的影响。方法:选用雄性SD大鼠,经右颈静脉插管引流,尾动脉插管灌注建立CPB(CBP组),转流时间l h,建立CPB动物模型。随机分为CPB模型组、CPB模型+GM1组、输血组和假手术组,每组10只。脑组织匀浆测定结构型一氧化氮合成酶(cNOS)、诱导型一氧化氮合成酶(iNOS)和NO活性。结果:CPB组脑组织中的iNOS活性由正常的(16.47±2.52)pmol/mg.prot-1.min-1增加到(69.84±8.73)pmol/mg.prot-1.min-1(P<0.01),CPB模型+GM1组则增加到(57.86±7.79)pmol/mg.prot-1.min-1(P<0.01),与CPB组比较差异有统计学意义(P<0.05);CPB组脑组织中的cNOS活性由正常的(57.74±8.78)pmol/mg.prot-1.min-1下降到(32.67±6.61)pmol/mg.prot-1.min-1(P<0.01),CPB模型+GM1组则下降到(42.72±7.23)pmol/mg.prot-1(P<0.05),与CPB组比较差异有统计学意义(P<0.05);CPB组脑组织中的NO含量由正常的(258.91±48.17)pmol/mg.prot-1增加到(713.74±98.43)pmol/mg.prot-1(P<0.01),CPB模型+GM1组则增加到(523.74±87.36)pmol/mg.prot-1(P<0.01),与CPB组比较差异有统计学意义(P<0.05)。结论:CPB脑损伤与iNOS产生大量的NO导致的神经毒性作用有关;GM1在一定程度降低了脑中活化的iNOS含量,减少了NO的分泌释放,具有一定的脑保护作用。     

Anti-tumor angiogenesis with a recombinant ag43/FGFR1 chimeric protein as a model antigen

In order to investigate the anti-tumor angiogenesis activity with a recombinant Ag43/FGFR1 chimeric protein(AF)vaccine in a mouse H22 hepatoma model,tumor volume and survival rate of the mice were studied at a 3-day interval.Microvessel density(MVD)was detected by immunohistochemistry.The endothelial deposition of autoantibodies within tumor tissues was examined by immunofluorescent staining,and anti-FGFR1 antibody-producing B cells(APBCs)were tested by enzyme-linked immunospot(ELISPOT)assay.Compared with t...     

应用生存分析寻找急性髓性白血病治疗前和治疗反应预后因素

对１２８例急性髓性白血病患者的化疗前和化疗反应因素的研究表明，诱导化疗反应比化疗前因素的预后意义更突出。影响缓解的因素包括年龄、脾肿大、诱导期血小板低于６０×１０￣９／Ｌ日数比例（ｐＣ＜６０日数比）、诱导期体温高于３８℃日数比例（体温＞３８日数比）和白细胞低于１×１０￣９／Ｌ日数比例（ＷＢＣ＜１日数比）。影响生存的因素则是诱导期平均最低白细胞计数、ＭＢＤＩ、ＰＣ＜６０日数比和体温＞３８日数比。ＰＣ＜６０日数比比ＷＢＣ＜１日数比更明显地与诱导化疗剂量相关。对诱导化疗反应良好的特征为ＭＢＤＩ＞６０％、平均为１４％的ＷＢＣ＜１日数比以及ＰＣ＜６０日数比＜２０％。     

高效液相色谱法测定人血清卡铂浓度

卡铂(carboplatin)又名碳铂,为二代铂族抗癌药物,具有与顺铂相似的抗瘤谱和作用强度,但其副作用较顺铂低,已在临床广泛使用.测定卡铂血药浓度有衍生化法和超滤法,但前者操作较繁琐,后者处理血样需特殊装置不易普及[1-3].为探讨该药的体内过程及剂量一效应关系,笔者建立高效液相色谱法测定人血清中卡铂浓度,报道如下.     

ATP modulation of large conductance Ca-activated K channels via a functionally associated protein kinase A in CA1 pyramidal neurons from rat hippocampus

Using inside-out configuration of patch clamp techniques, ATP modulation of BK(Ca) channels was studied in hippocampal CA1 pyramidal neurons of adult rat. Intracellular ATP application markedly increased BK(Ca) channel activity, and this ATP-produced increase in BK(Ca) channel activity was characterized by a higher opening frequency with no changes in channel open times. In the presence of specific inhibitor against protein kinase A, H-89, ATP did not induce any increase in the channel activity. Furthermore, adding H-89 after addition of ATP reversed the modulation produced by ATP. In contrast, protein kinase C inhibitor chelerythrine exerted no apparent effects on ATP-induced channel activation. The present study suggests that BK(Ca) channels from hippocampal CA1 pyramidal neurons could be modulated by ATP via a functionally associated protein kinase A-like protein.     

Role of vascular endothelial growth factor in neuronal DNA damage and repair in rat brain following a transient cerebral ischemia

The antisense knockdown technique and confocal laser scanning microscopic analysis were used to elucidate vascular endothelial growth factor (VEGF) induction and its effect on DNA damage and repair in rat brain following a transient middle cerebral artery occlusion. Immunohistochemical study and in situ hybridization showed that the expression of VEGF and its mRNA was enhanced in the ischemic core and penumbra of ischemic brain. Western blot analysis further illustrated that VEGF induction was time-dependently changed in these areas. Double-staining analysis indicated that VEGF-positive staining existed in the neuron, but not in the glia, and it colocalized with excision repair cross-complementing group 6 (ERCC6) mRNA, a DNA repair factor. VEGF antisense oligodeoxynucleotide infusion reduced VEGF induction and resulted in an enlargement of infarct volume of the brain caused by ischemia. Moreover, it also increased the number of DNA damaged cells and lessened the induction of ERCC6 mRNA in ischemic brains. These results suggest that the induction of endogenous VEGF in ischemic neurons plays a neuroprotective role probably associated with the expression of ERCC6 mRNA.     

线粒体脑肌病—CPEO征候群(附2例报告)

报告2例线粒体脑肌病之慢性进行性眼外肌瘫痪(CPEO)型的临床相关实验室检查及分子遗传学DNA组化分析所见,对线粒体脑肌病之诊断及若干临床有关问题进行讨论。