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981.
982.
Effects of recombinant human growth hormone on acute lung injury in endotoxemic rats 总被引:3,自引:0,他引:3
C. Yi S. R. Wang S. Y. Zhang S. J. Yu C. X. Jiang M. H. Zhi Y. Huang 《Inflammation research》2006,55(11):491-497
983.
In Xenopus, localized factors begin to regionalize embryonic fates prior to the inductive interactions that occur during gastrulation. We previously reported that an animal-to-vegetal signal that occurs prior to gastrulation promotes primary spinal neuron fate in vegetal equatorial (C-tier) blastomere lineages. Herein we demonstrate that maternal mRNA encoding noggin is enriched in animal tiers and at low concentrations in the C-tier, suggesting that the neural fates of C-tier blastomeres may be responsive to early signaling from their neighboring cells. In support of this hypothesis, experimental alteration of the levels of Noggin from animal equatorial (B-tier) or BMP4 from vegetal (D-tier) blastomeres significantly affects the numbers of primary spinal neurons derived from their neighboring C-tier blastomeres. These effects are duplicated in blastomere explants isolated at cleavage stages and cultured in the absence of gastrulation interactions. Co-culture with animal blastomeres enhanced the expression of zygotic neural markers in C-tier blastomere explants, whereas co-culture with vegetal blastomeres repressed them. The expression of these markers in C-tier explants was promoted when Noggin was transiently added to the culture during cleavage/morula stages, and repressed with the transient addition of BMP4. Reduction of Noggin translation in B-tier blastomeres by antisense morpholino oligonucleotides significantly reduced the efficacy of neural marker induction in C-tier explants. These experiments indicate that early anti-BMP signaling from the animal hemisphere recruits vegetal equatorial cells into the neural precursor pool prior to interactions that occur during gastrulation. 相似文献
984.
Association of the envelope glycoproteins of influenza virus with liposomes--a model study on viral envelope assembly. 总被引:9,自引:0,他引:9
Envelope glycoproteins of virus N (an avian influenza virus) were artificially associated with liposomes, using octylglucoside as detergent which could be readily removed by dialysis. The prepared liposomes, about 0.1-1 μm in diameter, contained specific hemagglutinin and neuraminidase activities. The total amounts of proteins incorporated into liposomes were about the same irrespective of the lipid composition. Similarly, the neuraminidase activity of liposomes remained unchanged by altering lipids. However, the hemagglutinating and cell-adsorbing properties of liposomes varied greatly depending on the composition of lipids. As both lipid and protein components can be altered, the present system may be elaborated for the study of protein-lipid interactions and functions of viral membranes. 相似文献
985.
Rh proteins vs Amt proteins: an organismal and phylogenetic perspective on CO2 and NH3 gas channels.
Rh (Rhesus) proteins are homologues of ammonium transport (Amt) proteins. Physiological and structural evidence shows that Amt proteins are gas channels for NH(3), but the substrate of Rh proteins, be it CO2 as shown in green alga, or NH3/NH4+ as shown in mammalian cells, remains disputed. We assembled a large dataset generated of Rh and Amt to explore how Rh originated from and evolved independently of Amt relatives. Analysis of this rich data implies that Rh was split from Amt first to emerge in archaeal species. The Rh ancestor underwent divergence and duplication along speciation, leading to neofunctionalization and subfunctionalization of the Rh family. The characteristic organismal distribution of Rh vs. Amt reflects their early separation and subsequent independent evolution: they coexist in microbes and invertebrates but do not in fungi, vascular plants or vertebrates. Rh gene-duplication was prominent in vertebrates: while epithelial RhBG/RhCG displayed strong purifying selection, erythroid Rh30 and RhAG experienced different episodes of positive selection in each of which adaptive evolution occurred at certain time points and in a few codon sites. Mammalian Rh30 and RhAG were subject to particularly strong positive selection in some codon sites in the lineage from rodents to human. The grounds of this adaptive evolution may be driven by the necessity to increase the surface/volume ratio of biconcave erythrocytes for facilitative gas diffusion. Altogether, these results are consistent with Rh proteins not being the orthologue of Amt proteins but having gained the function for CO2/HCO3- transport, with important roles in systemic pH regulation. 相似文献
986.
大鼠内毒素休克肝线粒体Ca^2+,Mg^2+含量变化与线粒体损伤 总被引:5,自引:1,他引:5
本文在大鼠内毒素休克模型上,观察了肝线粒体结构和功能与Ca^2+,Mg^2+含量变化的关系。结果发现:休克动物肝组织和线粒体的Ca^2+浓度升高,Mg^2+浓度下降与正常对照组相比有非常显著性差异;用图像分析仪定量分析显示线粒体结构明显受损,线粒体标志酶之一-琥珀酸脱氢酶(SDH)活性明显下降,本文认为组织和线粒体内Mg^2+/Ca^2+比值下降是休克细胞不可逆损伤重要因素之一。 相似文献
987.
988.
将人工合成的恶性疟原虫杂合74肽抗原基因克隆到表达载体pWR450-1中,获得了重组质粒pWRC,将其先转化到减毒伤寒沙门氏菌LB5000修饰后,再转化到SL3261,得到重组减毒鼠伤寒沙门氏菌SL3261(pWRC)。pWRC在SL3261中以β-半乳糖苷酶-杂合多肽抗原C融合蛋白(GZ-C)形式表达,表达量约占菌体蛋白总量的11.3%.Westernblot及免疫双扩散显示GZ-C可与兔抗GZ-C抗原的免疫血清发生特异反应。GZ-C识别兔抗GZ-C及鼠抗恶性疟原虫抗血清的ELISA滴度分别为1:3200及1:5120。初步结果表明SL3261(pWRC)表达的GZ-C具有抗原性。连续传代SL3261(pWRC)未见质粒pWRC丢失及对宿主有明显的毒性。 相似文献
989.
Transmission data for 4-MV bremsstrahlung beams have been measured with a combination of lead and aluminum attenuators. From these data, the original energy spectra have been reconstructed using an iterative least-squares technique, previously evaluated by simulation studies. The spectra on the central axis for three similar 4-MV linear accelerators indicated no significant differences. When studying the spectra at 5 degrees and 9 degrees off the central axis, that at 9 degrees showed the expected increase of low-energy photons. All these spectra showed a maximum photon energy of 4.5 +/- 0.2 MeV. When the magnetron power was reduced, the spectrum on the central axis shifted to lower energies and the maximum photon energy decreased to 3.5 +/- 0.2 MeV. The result of this experimental study confirms the conclusions from the previous stimulation, that the numerical technique for analysis of transmission data can accurately represent 4-MV bremsstrahlung spectra and detect differences in energy distribution with changes in machine tuning and position in the radiation field for a 4-MV bremsstrahlung beam. 相似文献
990.
A new haplogroup pattern displayed in Fujian Han in China 总被引:1,自引:0,他引:1
Yu M Zhang Y Xue Y Chen F Wang Q Huang X Wang B Yu Y Liu A Ma L Shi R Lu F Shi Z Zhang Y Cheng W Ai Q Xu F Huang C Chen B Yang H Kang X Sun Y Zhang G Li P Fu S 《Journal of human genetics》2002,47(2):95-98
Human Y-chromosomal binary polymorphisms have been considered to preserve the paternal genetic legacy and provide evidence
on human evolution and the genetic relationships among and demographic history of different populations. To reveal the genetic
origin and immigration of the Fujian Han, 13 binary markers on the Y chromosome were used to screen Fujian Han by allele-specific
polymerase chain reaction. The results indicated that the M9G marker was highly prevalent (96.20%), suggesting a significant genetic drift. In addition, M122C frequency was only 22.78%, and M45A and M103T were default. The distinctive haplogroup frequencies (H1, H5, and H6/7/8) imply that the haplogroup pattern is a relatively ancestral and interim type.
Received: October 13, 2001 / Accepted: December 3, 2001 相似文献