持续压力超负荷对兔左心室细胞凋亡的影响

OBJECTIVE: To study the effect of chronic pressure overload on the apoptosis of the left ventricle myocytes in rabbits. METHODS: Rabbit models of chronic pressure overload-induced heart failure were prepared in which dynamic changes of apoptotic myocytes in the left ventricle were observed by way of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay. RESULTS: Only a few apoptotic cells was observed in the sham-operated group, while in the experimental group, the apoptotic left ventricle myocytes significantly increased after operation, presenting a peak level between day 3 and day 7. Seven days after the operation, the apoptotic myocytes began to decrease and till day 14, the apoptotic cell number had been smaller than that measured on day 1. When signs of heart failure set in, the apoptotic myocytes were again increased (P<0.001). CONCLUSION: During chronic pressure overload, myocyte apoptosis in the left ventricle is elevated at the early stages and undulates subsequently, with the peak occurring before hypertrophy is obvious.     

Digital archive center: implementation for a radiology department.

OBJECTIVE. In this article, we describe the implementation of a digital archive center for a radiology department in a 700-bed teaching hospital. MATERIALS AND METHODS. The archive center consists of two identical archive systems, each comprising five components: an archive server, a data-base server, an optical disk library, a stand-alone optical disk drive, and a communication network. An image management system controls the image traffic from acquisition devices to display stations. A fault-tolerant mechanism was built into the archive center to achieve a 100% uptime. RESULTS. The center has been in operation for over 6 months. We have not experienced a single total system failure during this period. It currently archives all digital images from three MR units and four CT scanners and selected images from three computed radiographic systems and two laser film digitizers. The center archives between 1.5 and 2.0 gigabytes of images per workday. CONCLUSION. With its built-in fault-tolerant mechanism, we believe that the implemented archive center is very reliable and is suitable for a radiology department to archive its digital images.     

慢性肝病和肝癌中HCV核心区三种寡肽抗原的抗体检测

检测１４８例慢性肝病和肝癌患者血清中丙型肝炎病毒核心区３种寡肽抗原的抗体，结果显示其阳性率在慢性迁延型肝炎、慢性活动型肝炎、肝炎肝硬化和原发性肝癌患者分别为７．７％，２３．１％，，２３．５％和１６．３％。在抗体阳性者中８０％以上有乙型肝炎病毒感染证据，提示我国ＨＢＶ和ＨＶＣ重叠感染较为常见。     

高血压病人药物治疗期间动态血压变化

目的评价高血压病人药物治疗期间２４ｈ动态血压变化。方法２６例住院的高血压病人经药物治疗４周连续３ｄ随测血压，血压正常后进入本研究。治疗前后进行２４ｈ动态血压监测。结果患者随测血压（８～９ＡＭ，３～４ＰＭ）血压恢复到正常水平，但动态血压显示在一段时间内（６～８ＡＭ，６～１１ＰＭ）平均收缩和舒张压仍明显高于正常人平均水平（Ｐ＜０．０１），而该时间段易被临床医生忽视。结论随测血压不能实际全面反映高血压病人药物治疗的疗效，２４ｈ动态血压的监测可以正确评价高血压病人药物治疗的效果并根据高血压分布的时间来调整降压药的种类和剂量。     

Biomechanical evaluation of spinal stability after disc injury in a porcine model

The biomechanical effects of disc injury on flexion-extension were investigated in nine fresh porcine functional spinal units. Under 12 N-m in extension and 6 N-m in flexion, the range of motion among intact specimens were 23.11 degrees -33.29 degrees (mean = 26.07 degrees , SD = 3.25 degrees ), which shifted to 28.04 degrees -38.46 degrees (mean = 31.69 degrees , SD = 3.50 degrees ) when discs were injured. Similarly, the neutral zones among intact and disc injured specimens were 4.88 degrees -12.75 degrees (mean = 9.32 degrees , SD = 2.60 degrees ) and 9.50 degrees -24.43 degrees (mean = 16.80 degrees , SD = 4.69 degrees ) respectively. To obviate the differences between tested specimens, the changes of neutral zone and the range of motion after disc injury were normalized and expressed as ratios as compared to the same items before disc injury. The neutral zone ratio ranged from 1.29 to 3.18 (mean = 1.87, SD = 0.55, p < 0.01). For range of motion, it ranged from 1.13 to 1.42 (mean = 1.22, SD = 0.09, p < 0.01). The change of flexibility coefficient, however, was statistically insignificant in either flexion or extension. The model presented defines the range of motion, the neutral zone as well as the flexibility coefficient under a specific load. It suggests that major spinal instability can be elicited by a minimum flexion-extension moment, as reflected by a profound change of the neutral zone, after disc injury.     

变应性亚败血症33例报告

本文报告３３例变应性亚败血症，均以发热起病，伴有一过性皮疹和／或关节、肌肉酸痛，实验室检查以白细胞总数明显增高、血沉显著增快和粘蛋白增多为主要特点。本组病例初诊误诊率为８２．８％，起病至确诊时间平均４８ｄ，提示本病早期确诊较难，应提高对本病的充分认识。     

Passive exercise system: effect on muscle activity, strength, and lean body mass.

There is little information concerning the effects of passive exercise training in healthy humans. This prompted an investigation to evaluate muscle activity and the associated changes in strength and lean body mass resulting from a passive exercise program. Twenty-eight healthy volunteers, aged 26 to 44 years, participated in this six-week study. Lean body mass changes were evaluated by tetrapolar bioelectric impedance measurements, strength changes by isokinetic strength evaluation, and muscle activity by surface EMG techniques. Reproducible muscle activity was recorded in all three muscles studied during two of three preselected exercises. This involuntary muscle activity was attributed to fusimotor and postural reflexes. No significant change (p greater than .05), however, occurred in muscle strength or lean body mass at the end of the six weeks. Lack of these physiologic changes in light of the documented muscle activity is attributed to insufficient training effect.     

巢湖水有机提取物致鱼红细胞DNA损伤的初步观察

目的　研究巢湖水有机提取物致突变性。方法　单细胞凝胶电泳技术测定鱼红细胞DNA损伤效应。结果　巢湖源水引起慧星细胞的百分率最高 (5 7.2 5 % ) ,滤前水最低 (2 7.6 3% ) ,出厂水经过二次加氯后慧星细胞的百分率有所上升 (4 4 .0 0 % )。结论　巢湖源水具有潜在致突变性 ,经混凝、活性炭吸附及沉淀处理后其DNA损伤作用有所下降 ,但氯化消毒可增加水中有机提取物的DNA损伤作用。     

Comparative Study on the Immunogenicity between Recombinant MS-Sj26GST Vaccine and Recombinant BCG-Sj26GST Vaccine in Schistosoma japonicum

TuberculosisandSchistosomiasisarethemajorcontagiousdiseaseswhicharethemostdangeroustothepeople’shealth Inordertogetridofthem ,wemustlookforamoreusefulvaccine Bythetech niquesofmolecularbiology ,2 6 0 0 0DaGlutathionStransferase (GST) genewasclonedintotheE coli MycobacteriumtransferringandexpressionvectorpBCG 2 0 0 0totransformittoMycobacteriumsmeg matismc2 15 5 (MS)andBCGseparatelyinordertoconstructrMS Sj2 6GSTvaccineandrBCG Sj2 6GSTvaccine Inthisstudy ,theBALB/cmicewereimmu niz…