清热抗感冲剂对小鼠IL-2水平影响的实验研究

目的:通过检测清热抗感冲剂对实验性免疫低下小鼠及正常小鼠血清IL-2水平的影响,探讨该冲剂对免疫功能的调节作用.方法:选取昆明种小鼠100只,雌雄各半,其中50只腹腔注射环磷酰胺3mg/0.2mL/只,每天给药1次,连续7天,造成免疫低下小鼠,然后随机分为模型组、中药对照组(银翘解毒片组)、西药对照组(γ-干扰素组)、小剂量组、大剂量组;其余50只随机分为正常空白对照组(即生理盐水组)、中药对照组、西药对照组、小剂量组、大剂量组.上述各组连续用药3天后,处死,采血,离心取血清,采用放免γ测量仪检测IL-2含量.结果:清热抗感冲剂能显著提高正常小鼠和注射环磷酰胺引起的免疫低下小鼠血清中IL-2生成水平(统计学处理P＜0.01).结论:清热抗感冲剂对小鼠的细胞免疫功能有明显的增强作用.     

C反应蛋白与白蛋白比值对川崎病早期丙种球蛋白抵抗的预测价值

目的 探讨C反应蛋白与白蛋白比值(CRP/ALB)对川崎病(KD)早期丙种球蛋白(IVIG)抵抗的预测价值。方法 将2020年1月至2023年6月成都医学院第一附属医院住院的118例病程≤5 d的KD患儿分为IVIG抵抗组和IVIG敏感组,分析患儿临床资料,采用多因素Logistic回归分析探究独立危险因素,ROC曲线探究CRP/ALB对KD患儿早期IVIG抵抗的预测价值。结果 IVIG抵抗组比IVIG敏感组有更高水平的白细胞计数(WBC)、CRP、CRP/ALB和更低水平的血红蛋白(HGB)。CRP/ALB升高和HGB降低是KD患儿早期IVIG抵抗的独立危险因素。CRP/ALB可预测KD患儿早期IVIG抵抗(AUC=0.664)。结论 CRP/ALB升高和HGB降低是KD患儿早期IVIG抵抗的独立危险因素,CRP/ALB可有效预测KD患儿早期IVIG抵抗。     

Microstructure Evolution of Al6061 Alloy Made by Additive Friction Stir Deposition

In this paper, the phase structure, composition distribution, grain morphology, and hardness of Al6061 alloy samples made with additive friction stir deposition (AFS-D) were examined. A nearly symmetrical layer-by-layer structure was observed in the cross section (vertical with respect to the fabrication-tool traversing direction) of the as-deposited Al6061 alloy samples made with a back-and-forth AFS-D strategy. Equiaxed grains were observed in the region underneath the fabrication tool, while elongated grains were seen in the “flash region” along the mass flow direction. No clear grain size variance was discovered along the AFS-D build direction except for the last deposited layer. Grains were significantly refined from the feedstock (~163.5 µm) to as-deposited Al6061 alloy parts (~8.5 µm). The hardness of the as-fabricated Al6061 alloy was lower than those of the feedstock and their heat-treated counterparts, which was ascribed to the decreased precipitate content and enlarged precipitate size.     

艾儒棣教授治疗气阴两虚证狼疮性肾炎经验

艾儒棣教授临床辨治狼疮性肾炎,认为病机乃肾阴虚为本,瘀毒为标;强调缓解期对气阴两虚证的治疗,蛋白尿的处理,重视预后调摄。     

中药官桂的应用现状及其考证

目的研究中药官桂的应用现状及对其进行考证。方法采用性状鉴别法、显微鉴别法、薄层色谱法、高效液相色谱法对所收集的官桂样品进行鉴别,采用文献检索法进行官桂的相关考证。结果通过鉴别,官桂样品并不是同一品种,且有同一企业来源的两批官桂样品并不是同一品种的现象;通过考证,反映了官桂的来源复杂、标准规范不统一,临床上用的“官桂”与商品“官桂”并不相同。结论本研究首次通过实例报道了官桂的应用现状,结论是官桂的应用很混乱,同名异物现象很严重,应当加以重视,加强研究,争取统一其标准;相关企业要严格执行本地区有关官桂的地方标准,做到正本清源。     

Association between Excessive Dietary Branched-Chain Amino Acids Intake and Hypertension Risk in Chinese Population

The dietary intake of branched-chain amino acids (BCAAs) has been reported to be associated with both elevated blood pressure (BP) and hypertension risk, while published findings were inconsistent, and the causality has never been well disclosed. We performed this prospective study aiming to find out the relationship between dietary BCAAs intake and hypertension risk in the Chinese population. A total of 8491 participants (40,285 person-years) were selected. The levels of dietary BCAAs intake were estimated using the 24-h Food Frequency Questionnaire. Associations of both BP values and hypertension risk with per standard deviation increase of BCAAs were estimated using linear and COX regression analysis, respectively. The hazard ratios and 95% confidence interval were given. Restricted cubic spline analysis (RCS) was used to estimate the nonlinearity. Both systolic and diastolic BP values at the end points of follow-up were positively associated with dietary BCAAs intake. Positive associations between BCAAs intake and hypertension risk were shown in both men and women. By performing a RCS analysis, the nonlinear relationship between BCAAs intake and hypertension was shown. As the intake levels of Ile, Leu, and Val, respectively, exceeded 2.49 g/day, 4.91 g/day, and 2.88 g/day in men (2.16 g/day, 3.84 g/day, and 2.56 g/day in women), the hypertension risk increased. Our findings could provide some concrete evidence in the primary prevention of hypertension based on dietary interventions.     

2型糖尿病男性患者血清骨膜蛋白水平与骨质疏松的相关性研究

目的 探索骨膜蛋白水平2型糖尿病男性患者骨质疏松的关系.方法 收集了2016年3月至2017年3月在我院门诊诊断为2型糖尿病男性病人90例,体检中心健康男性人群中随机选取30例作为正常对照组,记录患者的年龄、腹围、身高、体重,计算BMI,测定甘油三酯、总胆固醇、高密度脂蛋白、低密度脂蛋白、糖化血红蛋白,血清骨膜蛋白水平...     

First-Principles Study on the Adsorption Behavior of O2 on the Surface of Plutonium Gallium System

To deeply understand the adsorption process of oxygen on the surface of a plutonium gallium system and to reveal the chemical reaction mechanism at the initial stage of oxidative corrosion on the surface of plutonium gallium alloy at a theoretical level, the adsorption behavior of oxygen molecules on the surface of a plutonium gallium system was investigated by a first-principles approach based on density flooding theory. The results show that the molecular bond length increases and finally breaks when the surface oxygen molecule is adsorbed on the surface of plutonium gallium system and dissociates into two atomic states. The most likely adsorption position of oxygen molecules on the surface of plutonium gallium system is hole-site vertical adsorption with the adsorption energy size of 10.7 eV. The bonding between oxygen atom and surface is mainly due to the overlapping hybridization of Pu-6s, Pu-7s, Pu-6d, Ga-3d and O-2p orbitals. Oxygen molecules mainly interact with the atoms of the first layer on the surface of the plutonium gallium system. The oxygen atoms after stable adsorption are able to diffuse to the subsurface of the plutonium gallium system after overcoming the energy barrier of 16.7 eV and form a stable structure. The research results reveal the initial reaction process and adsorption law of oxygen on the surface of plutonium gallium system from microscopic level, which is helpful to further explore the surface corrosion prevention technology of plutonium gallium system and improve the reliability and safety of plutonium gallium alloy components.     

柱前衍生HPLC法结合固相萃取测定血浆中卡托普利

目的:建立测定血浆中卡托普利浓度的方法,用于卡托普利人体药动学及相对生物利用度研究。方法:以对溴苯甲酸甲基溴为衍生化试剂,固相萃取法从生物样本中提取纯化衍生化产物,以反相高效液相色谱法测定衍生物含量而确定卡托普利血药浓度。结果:卡托普利血药浓度在10～1000ng·mL~(-1)范围内线性良好,相关系数为0.9987,测得平均回收率为98.65％,日内、日间RSD均小于10％。结论:本方法简便、准确、干扰小,适合用于卡托普利血药浓度监测及药动学研究。     

三七总皂苷调控PI3K/AKT信号通路预防糖尿病大血管病变的机制

目的从PI3K/AKT信号通路研究三七总皂苷(panax notoginseng saponins,PNS)预防糖尿病大血管病变的机制。方法雄性SD大鼠分为对照组、模型组和治疗组,模型组和治疗组大鼠高脂饲料喂养6周后给予链脲佐菌素单次腹腔注射(30 mg·kg^-1体质量)建立2型糖尿病模型,成模后治疗组给予PNS灌胃干预(100mg·kg^-1体质量)。血糖仪检测大鼠空腹尾静脉血糖(FBG),ELISA法测定血清空腹胰岛素(FINS)和糖化血红蛋白(GHbA1c)水平,苏木素-伊红(HE)染色观察腹主动脉病理形态改变,荧光定量PCR检测腹主动脉标记物MMP2、MMP9、CD34、VEGF及PI3K、AKT mRNA相对表达,Western Blot法观察PI3K和AKT蛋白表达情况。结果模型组和治疗组大鼠在STZ注射造模后,FBG均显著高于对照组(P<0.01和P<0.01),两组间差异无统计学意义(P>0.05)。模型组和治疗组血清INS均显著高于对照组(P<0.01和P<0.01),治疗组INS显著低于模型组(P<0.05)。HE染色显示,与对照组比较,模型组动脉壁变薄,平滑肌细胞增多,排列紊乱,内膜不光滑;治疗组较模型组明显改善。模型组MMP2、MMP9表达较对照组显著上调(P<0.05和P<0.01),治疗组MMP9较模型组显著下调(P<0.01)。模型组CD34、VEGF表达较对照组显著上调(P<0.05和P<0.01),治疗组CD34较模型组显著下调(P<0.05)。模型组大鼠PI3K表达较对照组显著上调(P<0.05),治疗组PI3K表达较模型组显著下调(P<0.05)。模型组PI3K蛋白、p-AKT蛋白表达均显著高于对照组(P<0.01和P<0.01),治疗组PI3K、p-AKT均显著低于对照组(P<0.01和P<0.05),模型组p-AKT/AKT显著高于对照组(P<0.05),治疗组p-AKT/AKT较模型组有显著降低趋势,且与对照组差异无统计学意义。结论PNS可能通过下调PI3K/AKT信号通路预防糖尿病大血管病变。