HMME在不同溶液中光漂白反应动力学规律

目的 分析国产光敏剂血卟啉单甲醚（HMME）在不同溶液中光漂白反应动力学规律，加深对光漂白反应复杂反应过程的理解，并为HMME-PDT治疗微血管病变的数学建模研究提供实验依据和参数。 方法 根据光化学反应动力学原理，从理论上推导出在单纯溶液和复杂溶液（含可被光氧化的底物）中，单态氧介导的光漂白反应动力学方程，通过对实验测定的漂白数据（不同时间点的HMME浓度）进行拟合，进一步验证理论推导出光漂白反应动力学方程。 结果 HMME在单一溶液体系（PBS、DMSO）中的光漂白符合1级反应动力学过程，在含底物的复杂体系（白蛋白缓冲液、细胞悬液）中符合2级反应动力学过程。 结论 HMME在不同溶液体系中的光漂白遵循不同的反应动力学规律。根据化学反应动力学原理进行光漂白等复杂光化学反应过程的数学模拟是可行的，并有利于对复杂光化学反应过程的深入理解。     

法洛三联症并发薄基底膜肾病一例

法洛三联症与薄基底膜肾病均非少见病,但两者发生于同一个体却十分罕见,我科曾收治1例,报告如下.     

腮腺外面神经颊支的应用解剖研究

目的:观测面神经颊支出腮腺后的各级分支,为相关外科手术提供解剖学依据。方法:10例(20侧)防腐人头标本,描述面神经颊支出腮腺后的走行、定位及层次。结果:面神经颊支出腮腺后分为2～4支,均走行在SMAS筋膜下;颊Ⅰ、颊Ⅱ恒定出现,其一级分支距腮腺导管分别为(1.1±0.5)cm及(0.5±0.3)cm,且分别向前走行(1.3±0.9)cm及(1.8±0.5)cm后发出二级分支。结论:面神经颊支较为恒定,在面瘫治疗中健侧面神经颊支的二级分支可以作为受体神经,可不损伤面神经颊支主干。面神经颊支的分布及走行也可指导除皱术、扩张器植入、瘢痕切除等手术的剥离范围,不致产生并发症。     

大鼠重度烫伤后早期心肌组织内缺氧诱导因子-1α表达的变化

目的　观察烫伤大鼠伤后早期心肌组织中缺氧诱导因子 1α(HIF 1α)mRNA和蛋白的表达变化 ,探讨其意义。　方法　制作 4 0 %TBSAⅢ度烫伤大鼠模型 ,设正常对照组及伤后不同时相组。取心脏解剖左、右心室 ,采用逆转录聚合酶链式反应 (RT PCR)法检测HIF 1αmRNA的表达 ,采用蛋白免疫印迹法测定其蛋白水平的变化。　结果　正常大鼠左、右心室肌HIF 1αmRNA、蛋白表达结果有所不同。大鼠烫伤后 ,心肌组织内HIF 1αmRNA水平、蛋白表达量明显升高 (P <0.0 1)。结论　正常状态下 ,大鼠左心室肌对缺血缺氧耐受能力较右心室肌强。严重烫伤可以诱导大鼠心肌细胞HIF 1α表达增加 ,介导心肌保护效应     

6～13岁儿童先天性髋脱位的手术治疗体会

儿童先天性髋脱位是一种较常见的畸形,治疗方法很多,疗效不一,尤其对较大儿童髋脱位尚无理想方法。我科1998年至2006年采用髋臼加盖成形和股骨粗隆下旋转截骨联合治疗6~13岁的发育性髋脱位共计17例,21个髋关节,随访15例,19个髋关节。现报告如下。1资料与方法1.1临床资料本组17例,21个髋关节,其中男性4例,6个髋关节;女性13例,15个髋关节。左侧9例,右侧4例,双侧4例。随访2~8年,平均4.8年。髋脱位可分为三度:Ⅰ度脱位属半脱位;Ⅱ度脱位,股骨头已脱出髋臼或髋臼上缘;Ⅲ度脱位指股骨头向上移位较高,达髂翼处。本组21例中Ⅰ度3例,Ⅱ7例,Ⅲ度11例。…     

美托洛尔控释片治疗中青年高血压病的临床观察

高血压病治疗的药物选择是临床医生需要综合考虑的问题，现将我院应用美托洛尔控释片对中青年高血压病进行治疗的观察结果和体会总结如下。     

Immature Megakaryocytes in the Mouse: Morphology and quantitation by acetylcholinesterase staining

Three types of immature megakaryocytes, detected by their morphological properties, have been characterized in bone marrow of normal C57BL/6 mice. Morphological classification of these cells was carried out by determining (1) presence and relative amount of acetylcholinesterase, (2) cell size, (3) nuclear/cytoplasm ratio, and (4) nuclear shape. The immature megakaryocytes were classified as: (A) cells distinguished by a round nucleus (10.6 +/- 1.1 mu diameter; mean +/- SEM), which had the highest nucleus / cytoplasm ratio and lowest content of acetylcholinesterase; (B) cells with an indented nucleus (13.0 +/- 1.9 mu diameter), which had increased acetylcholinesterase content and reduced nucleus/cytoplasm ratio compared to the round-nucleus cell type; and (C) lobed-nucleus cells (14.5 +/- 2.9 mu diameter), which showed further increase in acetylcholinesterase content and reduction in nucleus/cytoplasm ratio. Increased numbers of immature megakaryocytes were detected, indicating that a proportion of these cells are undetected using conventional staining techniques. Based on the observed alterations in size, acetylcholinesterase content, and nuclear complexity, it was concluded that these cells constitute part of a progressive maturation sequence intermediate between the progenitor cell (CFU-Mk) and mature easily recognizable megakaryocytes.     

Nitric oxide synthase inhibitor,nitro-iminoethyl-L-ornithine,reduces ischemia-reperfusion injury in rabbit skeletal muscle

Nitric oxide (NO), originally identified as the mediator of endothelial-dependent relaxation of vascular smooth muscle, is now known to also have cytotoxic effects under certain conditions. Thus, we have investigated the effects of inhibition of NO synthesis on ischemia/reperfusion injury in the rabbit rectus femoris muscle. Three and a half hours of ischemia and 24 hours of reperfusion resulted in a 56% loss of viability. In muscles receiving an infusion of the nitric oxide synthase inhibitor, L-NIO (30 μM), the loss of viability was reduced to 15%. Post-ischemic blood flow was increased in muscles receiving a saline infusion, whereas there was a marked decrease in blood flow for at least the first 60 minutes of reperfusion in muscles treated with L-NIO (30 μM). The increase in myeloperoxidase levels (indicative of neutrophil accumulation) following 24 hours of reperfusion was attenuated with L-NIO infusion by approximately 50% and the reperfusion-induced edema was also attenuated in L-NIO treated muscle. These findings suggest that endogenous NO production during ischemia/reperfusion injury may be deleterious to muscle survival. © 1994 Wiley-Liss, Inc.     

神效止痛膏的镇痛作用

用二甲苯所致的急性炎症模型观察了神效止痛膏的抗急性炎症作用．用扭体法、热板法观察了神效止痛膏对小鼠的镇痛作用．结果表明，神效止痛膏有很好的镇痛作用．     

车载苯胺泄漏事故造成水井污染的监测分析

2003年12月30日一辆装载7.28t苯胺的槽车从宁波开往温州的路上在甬台温高速公路温岭市境内翻车,造成5.3t左右的苯胺泄漏在高速公路旁的环境中,除少量的液体被清除外,绝大部分渗入地下,造成了水井污染.从12月31日开始我们对周围的水井开展了监测,现将监测结果分析如下: