抗肿瘤药物的研究 ⅩⅥ.Sb-71与几种金属盐合并治疗小白鼠Ehrlich腹水癌的作用

本文研究了sb-71与几种金属盐合并治疗小白鼠Ehrlich腹水癌的疗效,結果发現用硫酸銅3毫克/公斤、硫酸鋅20毫克/公斤、硫酸鎂30毫克/公斤、氯化鎂30毫克/公斤及氯化銅2.5毫克/公斤与sb-71 30毫克/公斤合并治疗小白鼠Ehrlich腹水癌时,疗效无明显变化,但硫酸銅与sb-71合并治疗組的动物一般情况較好,腹水出現較晚、腹水量亦少。用上述金属盐与sb-71 15毫克/公斤合并治疗时发現硫酸銅1.5,3毫克/公斤能显著地提高sb-71对小白鼠Ehrlich腹水癌的疗效,动物的生存时間較单用sb-71組延长,腹水量、瘤細胞浓度、瘤細胞总数亦显著減少.硫酸銅本身无治疗作用;亦不影响sb-71的毒性.在sb-71 30毫克/公斤治疗小自鼠Ehrlich腹水癌的同时投給氯化鋅3,6毫克/公斤,后者明显地拮抗Sb-7l的疗效,动物生存时間明显地短于单用sb-71組。     

Digoxin- and Monensin-induced Changes of Intracellular Ca2+ Concentration in Isolated Guinea-pig Ventricular Myocyte

This study was undertaken to determine the possible mechanisms of actions of monensin and digoxin by using isolated guinea-pig ventricular myocytes. Since Ca²⁺ is the major signal for triggering contraction of cardiac muscle, the objective of this study was to determine whether monensin and digoxin affect the [Ca²⁺]_i of cardiac myocytes and if so is this effect due to an increase in [Na⁺]_i. Three different concentrations of digoxin (0.3, 1 and 3 μmol/l) and three different concentrations of monensin (0.3, 1 and 3 μmol/l) were used. Each treatment was monitored for two hours by using computerized fluoroscopy. Both digoxin and monensin increased the [Ca²⁺]_i and accelerated the onset time of [Ca²⁺]_i increase in a dose-dependent manner. Normal myocytes (loaded with fura-2 for 30 min before the treatment) were also compared with `weakened' myocytes (loaded with fura-2 for 3 h before the treatment to create a `weakened' condition). It was found that although 0.3 μmol/l monensin and digoxin did not change the [Ca²⁺]_i in normal myocytes, they increased the [Ca²⁺]_i in `weakened' myocytes. Finally, a Na<sup>+</sup>-free medium was used to demonstrate the effect of [Na<sup>+</sup>]<sub>o</sub> on both monensin- and digoxin-induced increases in [Ca<sup>2+</sup>]<sub>i</sub>. It was found that digoxin did not increase the [Ca<sup>2+</sup>]<sub>i</sub> in the Na<sup>+</sup>-free medium. Although monensin increased the [Ca<sup>2+</sup>]<sub>i</sub> in the Na<sup>+</sup>-free solution, this increase was not as large as in the Na<sup>+</sup>-containing medium. The results of the study led to the conclusion that the positive inotropic effect of digoxin depends on [Na<sup>+</sup>]<sub>o</sub>. However, monensin increases [Ca<sup>2+</sup>]<sub>i</sub> in Na<sup>+</sup>-dependent and -independent ways. An addition conclusion was that `weakened' myocytes are more sensitive to the monensin and digoxin treatment than normal myocytes.     

Multivariate Analysis of Pacemaker Patient Survivorship

Multiple risk factors of patient mortality need to be evaluated and combined to serve practical purposes. A mul-tivariate statistical model is illustrated here with a large data set and a simulated example. The piecewise parametric model is shown to be simple, efficient, and is suggested to be a good tool for routine usage. The approach should provide a more concise characterization of mortality in terms of multiple risk factors. The results of data analysis also provide a good reference for further explorations.     

国产槐花米成分研究(Ⅲ)槐花米丙素

中药槐花米內含数种化合物巳志于第一次报告中,第一种青黄色針晶系黄鹼索、实驗式为C_(29)H_(36)O_(17),熔点不定185-188°左右發泡完,198-208°發泡熔状透明如蜂房状的角質,210°左右变深棕黄色,245-250°分解上升,比原物質高4-5倍,经水解作用,衍生物装备,功能团测定,吸收光譜测定及R_f值求出与蘆艼不同暫名槐花米甲素.第二次报告槐花米內含無色六面体結晶,非黃鹼素,实驗式为C_(25(26))H_(41(42))O_9(分子量未测定),熔点为272-274°,系一种不含氮的中性物質,属高級醇系甾体.本报告专研     

抗肿瘤药物的研究——ⅪⅩ.几种药物对家兔Brown-Pearce癌的影响

本文介紹16个药物对家兔Brown-Pearce癌实驗治疗的結果。这16个药物是,Thio-TEPA,BCM,HN₂,MBAO,TEM,AT-16,AT-222,AT-290,AT-346,AT-581,3P,合-14,Sb-57,Sb-7l,AC-808和放线菌素K.实驗証明,氮芥类药物AT-581[邻双-(β-氯乙基)氨甲基苯丙氨酸二盐酸盐]对靜脉接种此肿瘤有明显的抑制效能。以0.7毫克/公斤靜脉注射每天1針共12天,能显著延长肿瘤家兔的平均生存时間,延长率为130%(P<0.001),对肺和肝脏的重量和轉移病灶数也有明显的抑制作用。以1毫克/公斤靜脉注射时,能使肿瘤全部抑制,肺、肝和腎脏的重量比对照組明显下降。其它15个药物的作用不显著。本文对实驗方法及疗效指标等問題也进行了討論。