实施责任护士竞聘制的做法与成效

目的 :营造良性竞争的机制和氛围 ,全面提高护理质量。方法 :通过资格认定、理论技术考试、民主测评及双项选择 ,由护理部聘任。结果 :较好地进行了人力资源的开发 ,竞聘上岗后的护士有责任感和使命感 ,工作积极主动 ,自觉地成为科室各项工作的带头人。加之岗位工资的兑现 ,更有利于护士长在科室开展工作 ,使科室各项护理工作质量显著提高。结论 :良性的竞争运行机制 ,使优秀护理人才脱颖而出 ,体现能级对应的原则 ,从而使护理质量不断提高。     

假体隆乳术后并发症的防治

目的探讨乳房假体隆乳术后并发症产生的相关因素及预防方法。方法对198例患者乳房假体隆乳术后1~17年出现的并发症进行分析,阐述防治措施,针对不同情况进行相应的处理。结果本组198例患者,其中92例患者随访半年至1年,除Ⅲ°包膜挛缩患者术后仍出现Ⅱ~Ⅲ°包膜挛缩外(包膜松解术后3例,包膜切除术后4例),余患者对治疗效果满意。结论乳房假体隆乳术后出现的并发症,多数是可以预防和治疗的,只要术中操作规范,对并发症及早准确的处理,是提高手术效果,减少并发症发生的有效方法。     

原位心脏移植30例临床分析

目的对30例心脏移植进行分析总结。方法30例患者中,24例的原发病为扩张型心肌病,4例为终末期瓣膜性心肌病,1例为终末期缺血性心肌病,1例为病毒性心肌炎行双心室辅助术后1个月。均在体外循环下行原位心脏移植术,3例采用标准心脏移植术式,27例采用双腔静脉吻合心脏移植术式,体外循环时间(75±24)min,主动脉阻断时间(72±8)min。术后采用环孢素A、霉酚酸酯和泼尼松预防排斥反应,根据血环孢素A的浓度调整CsA的用量。术后早期,每天行心肌内心电图及超声心动多普勒监测,以便早期发现急性排斥反应,必要时行心内膜心肌活检。结果30例患者中,术后死亡5例,3例死于低心排,1例死于感染所致的多器官功能衰竭,1例死于出血。术后并发症有低心排、心律失常、三尖瓣返流、右心衰竭、细菌和真菌感染、肾功能异常及血糖升高,上述并发症除导致死亡者外,其余经治疗好转;4例发生急性排斥反应,给予甲泼尼龙冲击治疗,并调整免疫抑制剂的用量后排斥反应逆转。结论术后并发症的预防和及时正确的处理,是心脏移植成功的关键,尤其是对感染、急性排斥反应、右心功能不全及肾功能异常的预防和处理。     

Isolation and differentiation of embryonic stem cells from BALB/c mouse

Objective To invest the efficient method which can culture and induce embryonic stem cells to neurocyte in vitro. Methods Isolate the blastula of 3.5 d from BALB/c species mouse. Culture the cells from inner cell mass (inner cell mass, ICM) which were isolated by mechanical method on the mouse embryonic fibroblaste cell (MEF) feeder layer or 0.1% gelatin coated dishes. The stem cells were identified by characterized morphology, alkaline phosphatase stain, differential potency in vivo and immunochemistry stain. The isolated cells were differentiated by serial induction method that mimicking the intrinsic developmental process of the neural system. Results The isolated cells were positive for alkaline phosphatatse and SSEA-1 (stage specific embryonic antigen 1). Moreover they were identified pluripotent by differentiation in vivo. Therefore the isolated cells presented the characters of ESCs. Then the isolated cells were able to differentiate into neurocytes in vitro. Conclusion Mouse embryonic stem cells isolation, culture and differentiation system has been established.     

BP神经网络及其在药学研究中的应用

BP神经网络是目前应用最广泛的人工神经网络模型，本文详细阐述了BP神经网络的原理和特点，并论述了其在药学研究中的应用。     

高龄人群口腔卫生保健情况调查

目的：了解高龄老人口腔卫生保健状况.方法：对80岁以上老年人口腔卫生知识问卷调查.结果：具有口腔健康行为的人群,其口腔健康状况良好.结论：加强口腔卫生保健知识教育,养成良好口腔行为习惯,对促进老年人口腔健康很重要.     

我国城市地下水水质污染状况研究进展

综述了我国城市地下水水质污染状况的研究进展     

Effects of Nephritis No.3 Recipe on Nitric Oxide,Nitric Oxide Synthase Secreted by Cultured Mesangial Cells in Rats and the Gene Expression of Inducible Nitric Oxide Synthase

Objective:To explore the effect of the Nephritis No.3(N-3)recipe on nitric oxide(NO),nitric oxide synthase(NOS)secreted by cultured mesangial cells(MC)and its gene expression of the in-ducible nitric oxide synthase(iNOS).Methods:The drug(nephritis No.3)-containing serum was preparedwith serum pharmacological technique,and then was applied to react on mesangial cells cultured In fetalcalf serum(FCS)and cells cultured in FCS plus lipopolysaccharide.To observe the secretion of NO andNOS and the gene expression of iNOS by means of RT-PCR.Results:Under the two kinds of culture con-ditions,the content of NO and NOS in the groups with drug-containing serum were higher than thosewithout drug-containing serum(P<0.05,P<0.01),and the expression of iNOS mRNA was up-regula-ted too.Conclusion:The N-3 could significantly promote the secretion of NO and NOS and the mRNA ex-pression of iNOS in rats.     

顽固性室性早搏的射频消融治疗

目的 :通过对 2 3例顽固性室性早搏 (简称室早 )的射频消融分析 ,探讨室早的心电图特点、消融方法及效果 .方法 :男性 16例 ,女性 7例 ,均为频发、药物难治、症状明显的顽固性单形性室早患者 ,年龄(4 4 4± 9 6 )岁 .采用起搏标测和激动顺序标测 ,前者以起搏时与室早QRS波形态完全相同点为消融靶点 ,后者以早搏时最早心室激动点为消融靶点 .2 3例中 17例室早起源于右室 ,4例起源于左室 .消融即刻成功率86 9% ,累积放电 (930 4± 72 5 )s,成功者行动态心电图检查记录消融前后室早数为 (5 6 71± 2 4 36 )次 / 2 4h和 (39± 5 2 )次 / 2 4h (P <0 0 1) .随访 (2 3 2± 12 3)月 ,未服任何抗心律失常药 ,无 1例复发及并发症发生 .结论 :射频消融可有效而安全地消除单形性室早 ,采用粗标激动顺序、精标起搏图形 ,多点、长时间、高功率放电可提高成功率 ,降低复发率 ,可作为症状严重、药物无效或不能耐受患者的治疗选择     

管状骨增宽牵引成骨组织形态学变化的实验研究

目的 探讨管状骨增宽牵引后新骨形成的变化。方法 在成年山羊的后肢放置2只增宽牵引器。实验组9只术后第8天牵引，对照组3只不牵引。牵引完毕后不同时期，各宰杀3只，评价管状骨增宽牵引后新骨形成的质和量。结果 实验组胫骨平均增宽7．83mm，X射线见牵引间隙逐渐变模糊，暴露侧有骨不连，非暴露侧成骨良好，牵引完毕3个月，未暴露侧新形成板层骨与原来胫骨融为一体，而暴露侧骨不连区为致密纤维组织。结论 管状骨增宽牵引成骨后，两侧牵引间隙成骨不一致，良好的血供，对新骨形成至关重要。