线宽增量法及其在生物材料发射光谱分析中的应用

作者利用交流电弧和光栅摄谱仪组装了能够绘制谱线轮廓曲线和准确测定谱线宽度的记录仪,用线宽增量法对生物材料(拟血清)中的主要成分Ca和微量元素Cu进行了测定。与黑度法相比,本实验结果的线性好,误差小,再现性有所提高。这些结果表明,用上述仪器装置和线宽增量法进行生物材料含量分析优于黑度法。     

雪旺细胞源运动神经营养蛋白对周围神经再生的影响

目的：研究雪旺细胞源运动神经营养蛋白对周围神经再生的影响。方法：６０只ＳＤ大鼠均分为３组，Ａ和Ｂ组分别在桥接于大鼠右侧坐骨神经断端之间的硅胶管内加入２６ＫＤ和５０ＫＤ的雪旺细胞源运动神经营养蛋白，Ｃ组加ＰＢＳ液作为对照。术后１～６月每隔半月测定实验侧坐骨神经功能指数，术后３月测定实验侧坐骨神经的电生理和形态学指标。结果：Ａ和Ｂ组实验侧坐骨神经的功能指数、电生理和形态学指标与Ｃ组比较，差别有非常显著性。结论：２６ＫＤ和５０ＫＤ雪旺细胞源运动神经营养蛋白对周围神经再生有促进作用。     

磷脂酶C与生殖

磷脂酶C(PLC)是磷脂酰肌醇信号转导途径中一个关键酶。目前确认的哺乳类PLC共有PLC β、PLC γ、PLC δ、PLC ε和最近新发现的PLC ζ 5种亚型 ,12种同工酶。不同亚型的结构、调控和组织分布各有差异。PLC在调控信号转导通路中的特性使其在顶体反应、卵子激活中发挥了巨大的作用。本文综述了PLC家族 5种亚型的结构、调控机制及PLC在男性生殖领域的相关内容 ,包括PLC引起卵子发生Ca2 + 振荡、激活卵子、促进胚胎发育等 ,并进一步探讨了PLC在临床上可能的应用前景。     

宫腔镜联合腹腔镜检查不孕症168例分析

目的探讨宫、腹腔镜联合检查不孕症的诊断价值.方法回顾性分析1999年6月～2003年10月宫、腹腔镜联合检查168例不孕症的临床资料.结果宫腔镜检查发现异常宫腔79例（79/168,47.0%）,其中子宫内膜增生、内膜息肉46例（46/79,58.2%）.腹腔镜检查发现盆腔器质性病变99例,其中慢性盆腔炎、子宫内膜异位症和多囊卵巢共85例（85/99,85.9%）;宫、腹腔镜检均正常15例,均异常39例.宫腔镜下行输卵管通畅检查,输卵管单侧或双侧不通90例（90/168,53.6% ）;腹腔镜检查输卵管单侧或双侧不通78例（78/168,46.4%）.结论宫腔镜联合腹腔镜检查不孕症能够提供准确的诊断依据.     

Nucleus A10 dopaminergic neurons in inbred mouse strains: Firing rate and autoreceptor sensitivity are independent of the number of cells in the nucleus

Inbred mouse strains have different numbers of midbrain dopaminergic neurons; for example, BALB/cJ mice have 20–25% more neurons than CBA/J mice. As the number of cells decrease, for example in Parkinson's disease and in animals with midbrain dopaminergic cell lesions, the activity of their remaining cells increases. The purpose of the present experiment was to determine whether the functional properties of dopaminergic neurons in the ventral tegmental area (nucleus A10) differ in inbred mouse strains which possess different numbers of cells. The firing rate and autoreceptor sensitivity of A10 dopaminergic cells were examined in the in vitro slice preparation in BALB/cJ, C3H/HeJ, CBA/J, and DBA/2J mouse strains. It was observed that the autoreceptors on mouse dopaminergic neurons exhibit pharmacological properties of dopamine autoreceptors; activation of the autoreceptor produced a marked inhibition (50–70%) in cell firing rate by quinpirole (10⁻⁸ M), LY-141865 (10⁻⁷ M), (+)-3-(3-hydroxyphenyl)-N-n-propyl-piperidine (10⁻⁶ M), propyl-norapomorphine (10⁻⁵ M) and dopamine (10⁻⁴ M), and this inhibition was blocked or reversed by specific dopamine D2 receptor antagonists [(−) sulphide and spiroperidol, 10⁻⁶ M]. The baseline firing rates of the A10 cells did not differ among the four inbred strains [range 2.5 ± 0.2 (C3H/HeJ)−3.4 ± 0.3 (CBA/J) spikes/s ±SEM], and there was no significant difference in autoreceptor sensitivity among the mouse strains as assessed either by superfused dopamine (inhibitory dose 50% ≈150 μM), or by superfused quinpirole (inhibitory dose 50% ≈10 nM). These data indicate that differences in A10 cell numbers of 30% do not significantly influence the baseline firing rate or autoreceptor sensitivity of the cells.     

精浆抗精子抗体阳性不育患者顶体酶、一氧化氮合酶及超氧化物歧化酶活力变化的研究

目的:研究精浆抗精子抗体(AsAb)阳性对精子顶体酶、精浆一氧化氮合酶(NOS)及超氧化物歧化酶(SOD)活力的影响。方法:精浆AsAb阳性不育者40例,对照组为40例正常生育男性。通过吸光度变化分别计算顶体酶活力(BAEE/ADH联合法)、NOS活力(氧化还原反应)、SOD活力(黄嘌呤氧化酶法)。结果:精浆As-Ab阳性组与正常生育组比较,精子顶体酶活力明显降低(P<0.01),NOS活力明显升高(P<0.01),精浆中SOD活力明显降低(P<0.01)。结论:精浆AsAb阳性引起不育可能与精子顶体酶、精浆中SOD及NOS活力改变有关。     

Radiopacity of dental materials using a digital X-ray system.

OBJECTIVES: Radiopacity is a desirable property for most intra-oral materials. There are established ISO and ANSI/ADA protocols for determining radiopacity using film-based radiography. However, these methods are not always followed by researchers. This study aims to adapt those procedures by using digital radiography, a simplified stepwedge, and examine the effects of target distance and exposure time choice. METHODS: One millimetre thick samples of three dental materials were prepared by placing the materials into a 1.00 mm thick washer sandwiched between two glass slides. The samples were digitally radiographed alongside a stepwedge of aluminum alloy 1100 with an X-ray unit at 70 kVp using five different target distance/exposure time combinations. For each combination, the grey scale values of various thicknesses of the stepwedge were converted into absorbencies and plotted against their thickness. These plots were then linearly regressed in order to correlate absorbance with a thickness of aluminum for each target distance/exposure time combination. The absorbencies of each sample were then converted into radiopacities using these correlations. RESULTS: The correlations between the absorbance of the stepwedge and its thickness were highly linear. This linearity allows the correlation to be accurately deduced from fewer data points than required by the ISO and ANSI/ADA protocols. Varying exposure time did not significantly affect the mean radiopacity measured at a target distance of 30 cm. Varying the target distance did not significantly affect the measured radiopacity as long as the samples were properly exposed. SIGNIFICANCE: A simplified, consistent digital method for determining radiopacity is presented.     

蚌埠医学院在校学生心理卫生调查

目的　了解蚌埠医学院在校学生心理状况 ,为素质教育提供服务。方法　采用症状自评量表 (SCL- 90 )对该校 1～ 4年级学生进行调查分析。结果　该校在校生有 5个因子得分高于全国大学生常模 ,高年级学生得分高于低年级 ,男女生在人际、抑郁、敌对、偏执等方面差异有显著性。结论　应针对大学生的心理特点 ,在大学院校进行心理健康教育。     

甲壳素涂层PGLA神经导管修复兔面神经缺损的实验研究

目的:探讨甲壳素涂层PGLA神经导管修复兔面神经缺损的效果。方法:成年雄性新西兰兔24只,无菌条件下切断双侧面神经下颊支,制成15mm的兔面神经下颊支缺损模型。左侧用甲壳素涂层聚丙交脂-乙交脂共聚物[poly(L-lactide-co-glycolide),PGLA]神经导管修复;右侧用翻转自体神经修复作为对照。术后5周、10周和14周行大体观察、电生理检查、组织学、电镜观察评价修复效果。结果:术后5周观察到神经导管中有新生轴索通过,再生神经发育不成熟;右侧自体神经修复近段有髓神经纤维均匀疏散分布,远段未见明显再生神经束形成。术后14周左侧再生神经已通过神经导管长入远端,电生理检查结果表明自体神经修复侧再生神经质量优于神经导管修复侧,差异有统计学意义(P<0.01)。自体神经修复再生纤维密度优于神经导管修复侧,差异有统计学意义(P<0.01),但自体神经修复侧近段神经髓鞘部分空泡样变性及脱髓鞘改变,远段再生神经纤维束形成少,面肌联带运动程度较导管修复侧严重。结论:甲壳素涂层PGLA神经导管能有效修复周围神经缺损,有望替代自体神经移植。