酒客乐对酒精性肝损伤大鼠血清微量元素含量的影响

目的 :观察酒客乐对酒精性肝损伤大鼠血清微量元素含量的影响。方法 :用白酒、吡唑、橄榄油的混合液制成酒精性肝损伤模型 ,酒客乐冲剂为防治用药 ,观察血清微量元素Cu、Zn、Se及Mn含量的变化。结果 :与正常组比较 ,肝损伤组血清Se、Cu、Zn含量下降 (P <0 0 1或P <0 0 5 ) ;而防治组血清Se、Cu、Zn含量得以回升 ,其中Zn含量升至 3 2± 0 2 4ug ml,明显高于损伤组 (P <0 0 1)。结论 :酒客乐可通过补充酒精性肝损伤大鼠某些血清微量元素含量间接发挥清除自由基 ,稳定生物膜的作用     

硅凝胶型乳房假体凝胶外渗的实验研究

目的 :研究硅凝胶型乳房假体植入大鼠体内后凝胶外渗对机体的影响。方法 :取 40只健康SD雌性大鼠分成A、B、C、D 4组 ,A、B、C 3组分别植入实验用完整的硅凝胶型假体、刺破的硅凝胶型假体及生理盐水充注型假体于大鼠背侧皮下 ,D组为空白对照。术前测出血液中有机硅的浓度。 6个月后取出假体观察 ,进行假体包膜组织学检查及大鼠血液中术后有机硅的浓度测定。结果 :A、B、C 3组纤维包膜组织学特征无明显差异 ,B组纤维包膜中光镜下发现外渗的凝胶颗粒。术后与术前 4组大鼠间血液中有机硅的浓度比较 ,差异均无显著性。结论 :完整的硅凝胶假体、人为刺破外壳的硅凝胶假体与生理盐水充注型假体的组织学反应无明显差异 ,外渗的凝胶局限在纤维包膜的内层 ,也不会随血液或淋巴到机体其他部位。     

生长激素对糖尿病及其微血管病变的影响

目的 :探讨生长激素对糖尿病及其微血管病变的影响。方法 :采用放射免疫法测定 6 7例Ⅱ型糖尿病患者及 18例正常人的血清生长激素 (GH)及胰岛素 (INS)水平。结果 :糖尿病合并微血管病变组 ,空腹生长激素 (FGH)明显高于正常对照组 (P <0 .0 1)及糖尿病无微血管病变组 (P <0 .0 1)。糖尿病无微血管病变组的FGH与正常对照组的FGH相比未见明显差异 (P >0 .0 5 )。餐后 2h的生长激素 (PGH)在 3组之间均无显著差异 (P >0 .0 5 )。FGH与HbA1c、空腹血糖 (FBS)呈正相关 (r=0 .5 46 ,P <0 .0 1;r=0 .437,P <0 .0 1) ,与UAER呈正相关 (r=0 .486 ,P <0 .0 1) ;FGH与病程无相关 (r=0 .2 2 ,P >0 .0 5 ) ,与FINS无相关 (r =- 0 .0 2 9,P >0 .0 5 )。结论 :GH水平的增高 ,与糖代谢紊乱有关 ,参与了糖尿病微血管病变的发生。     

儿童肾病综合征患者血小板和血浆von Willebrand因子的变化

目的：观察儿童肾病综合征患者血小板计数和血浆von Willebrand因子的变化,方法,采用ELISA法检测58例NS患儿血浆vWF及血小板计数,并与正常对照组进行比较,结果：NS患儿vwF水平及血小板计数均明显高于正常对照组,两组有显著性差异（P＜0．01）,结论,血浆vWF与血小板计同步升高与NS患儿的高凝状态及血栓形成有一定的关系。     

人类巨细胞病毒感染对人胚肺细胞HOXB1,HOXB5,HOXB6及HOXB9基因表达的影响

目的 :研究人胚肺 (HEL)细胞HOXB1,HOXB5 ,HOXB6及HOXB9基因的表达状态及人类巨细胞病毒(HCMV)感染对上述基因表达的影响。方法 :采用半定量逆转录 -聚合酶链反应 (RT -PCR)技术。结果 :①HEL细胞表达HOXB5和HOXB6 ,但不表达HOXB1和HOXB9;②HCMV感染后 ,HEL细胞HOXB6基因的表达上调 ,且被HCMV诱导表达HOXB9T ,而HOXB5基因的表达无明显改变 ,HOXB1基因仍旧不表达 ;③全反式维甲酸 (ATRA)能上调HCMV感染后HOXB9基因的表达 ,但对HCMV感染晚期HOXB6基因的表达有抑制作用。结论 :HCMV能诱导HOXB6和HOXB9基因表达异常 ,这可能在先天性HCMV感染导致胚胎发育畸形中起重要作用     

Incidence and risk factors of suicide among patients diagnosed with bladder cancer: A systematic review and meta-analysis

Background: Patients with bladder cancer have a high risk of suicide. This study aimed to assess how bladder cancer increases suicide risk and to identify the demographic and clinical factors associated with suicidal death among patients with bladder cancer. Methods: Literature search of MEDLINE, PsycINFO, Embase, Web of Sciences and Cochrane Library databases was conducted up to April 2020 to identify eligible studies related to the incidence and risk factors of suicide after bladder cancer diagnosis. Summary multivariate-adjusted risk estimates and their associated 95% confidence intervals (CIs) were calculated using inverse variance method with random or fixed-effect modeling. Results: Five retrospective cohorts comprising 563,680 patients with bladder cancer were included. Higher risk of suicide by 1.90-fold was observed among patients with bladder cancer (hazard ratio, HR = 1.90, 95% CI: 1.29–2.81; P = 0.001; I² = 81.2%), especially in those aged 70 years or older (HR = 1.36, 95% CI: 1.29–1.43; P < 0.001; I² = 0%), unmarried (HR = 1.72, 95% CI: 1.61–1.83; P < 0.001; I² = 0%), and those with regional bladder cancer (HR = 1.88, 95% CI: 1.10–3.21; P = 0.021; I² = 96.3%), compared with those without bladder cancer. Furthermore, gender and race were not associated with increased suicide risk among patients with bladder cancer. Conclusions: Suicide risk is increased among patients with bladder cancer, particularly those aged 70 years or older, unmarried and those with regional bladder cancer. Hence, early psychological support must be provided during the follow-up period of these special populations with a high suicide risk.     

“They Assume That You’re Not Having Sex”: A Qualitative Exploration of How Paediatric Healthcare Providers Can Have Positive Sexuality-Related Conversations with Youth with Disabilities

Sexuality and Disability - Every individual should have equal access to sexuality-related information and services. Regrettably, societal stigma revolves around the sexuality of youth with...     

股骨粗隆间骨折术后并发症及原因分析

目的回顾性分析股骨粗隆间骨折的术后并发症并分析其原因。方法 2008年1月~2012年12月,我院手术治疗股骨粗隆间骨折共295例,按Evans分型,Ia型26例,Ib型53例,Ic型97例,Id型52例,II型67例。其中DHS组59例,IMHS组80例,PFN组25例,GAMMA3组28例,INTERTAN组103例。随访3~36个月,对部分发生并发症的病例进行手术干预。结果内固定物切割6例,拉力螺钉退出2例,髋内翻、下肢短缩12例,感染6例,血管损伤2例,股骨头无菌性坏死2例,内植物远端骨折2例,部分经再次手术治疗治愈。结论手术治疗股骨粗隆间骨折,要结合骨折类型、患者全身情况合理选用内固定物,术中复位及操作技术的改进以及术后适度的功能锻炼,能够减少术中及术后并发症的发生。     

Bioactive glass serves as a substrate for maintenance of phenotype of nucleus pulposus cells of the intervertebral disc

The objective of the study was to investigate the capability of bioactive glass 45S5 to serve as a substrate for nucleus pulposus cells in vitro. Nucleus pulposus cells were isolated from adult rabbit discs and seeded onto bioactive glass. At selected time intervals, the cells and glass were evaluated. We found that the cells rapidly attached to the substrate, colonizing it within 12 h. By 21 days, they had formed a lawn of cells over the glass substrate. DNA measurements showed a progressive increase in cell number with time. The phenotype was maintained; the cells expressed aggrecan, and collagen type II and I, but were negative for collagen type X. CD44, a cell-surface glycoprotein that binds hyaluronate, was also expressed by these cells. Electron dispersive X-ray analysis and Fourier transform infrared spectroscopy revealed calcium phosphate-rich layer formation on the substrate surface. The results of this study suggest that nucleus pulposus cell proliferation may be an anchorage dependent event, and that the cells use the calcium phosphate-rich layer to facilitate cell adhesion, and subsequent proliferation. These findings point to the importance of the conditioned bioactive glass as a substrate for nucleus pulposus cells.