重庆地区胃,十二指肠疾病患者幽门螺杆菌感染的调查

本文采用分离培养、尿素酶试验及直接涂片革兰氏染色镜检等方法，对１７９８例胃镜受检者的幽门螺杆菌感染情况进行的调查表明，该菌的总感染率为４９．７％，其中浅表性胃炎、十二指肠溃疡、胃溃疡和浅表性胃炎合并十二指肠球炎患者的感染率分别为４７．３％、７５．９％、７０．９％和４９。４％，复合溃疡、胃癌、慢性胃炎、胆汁返流性胃炎和浅表性萎缩性胃炎患者的感染率分别为２７。３％、２３．５％、１５．４％、１３．２％和１２．５％。     

医院做好社会服务承诺制工作的体会

在医院加强行风建设中，从就诊群众需要、加强医院管理出发，把医院置于群众的监督中，认真推行社会服务承诺制，有利于医院提升社会形象，有利于医务人员自觉增强自身的责任感。在实行社会服务承诺制中，要采取一系列切实可行的措施，才能保证承诺制的顺利实施。     

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目的　探讨腹腔镜食道裂孔疝修补术的可行性及安全性。方法　回顾性分析 2 0 0 1年 3月至 2 0 0 3年12月天津南开医院行腹腔镜食道裂孔疝修补术 11例病人的临床资料。结果　 10例行裂孔疝修补后同时行胃底折叠术 ,其中 7例行Nissen胃底折叠术 ,3例行Toupet胃底折叠术 ,1例仅行裂孔修补术。术后症状完全缓解。食道下段压力由 (8 6± 2 4 )mmHg(1mmHg =0 133kPa)提高到 (18 2 0± 3 4 3)mmHg(P <0 0 1) ,2 4hpH值监测评分由 5 3 4± 39 7降低到 8 0 4± 2 12 (P <0 0 1) ,较手术前有明显改善 ,并达到正常范围。无手术并发症 ,无中转开腹及死亡病例。结论　对于食道裂孔疝 ,腹腔镜食道裂孔疝修补术是一种安全、有效的治疗方法     

腹腔镜在原因不明性腹水的诊断中的应用

为了探讨腹腔镜在原因不明性腹水的病因诊断中的价值，对１１例经过Ｂ超、ＣＴ、腹水各种检查等仍然未明确诊断，并除外有心肝肺肾的疾病引起的腹水，在全麻下做腹腔镜检查、并取病变组织做病理学检查，１１例患者全部被确诊，结果表明：腹腔镜检查是诊断原因不明性腹水的有效方法，且并发症轻，可作为腹水病因诊断的一种常规检查     

北京石景山地区孕妇女婴幼儿血铅动态研究

在严格的质量保证措施下，北京石景山地区２７０名孕妇及其婴儿进行了血铅的追瞎调查。结果显示：孕３月血铅水平为４５．０μｇ／Ｌ，分娩前血铅水平为６４．８μｇ／Ｌ，孕期血铅呈升高趋势，其中后３月升高明显，分娩前血铅约为孕３月血铅的１．５倍；脐带血铅水平为５１．９μｇ／Ｌ，其中有１０．４％的胎儿脐血铅高于１００μｇ／Ｌ；     

Effects of Vagal and Sympathetic Nerves on Transmembrane Potential of Cardiac Ventricular Cells of Toad

The effects of vagal and sympathetic nerves on the transmembranepotentials of cardiac cells of toad were observed by means of microelectrodetechnique.The vagal nerve was stimulated there would be an increase in restingpotential and acceleration in repolarization of action potential(AP).However,ifatropine was used before stimulation the above-mentioned phenomena woulddisappear.When the sympathetic nerve was stimulated the AP amplitudeincreased,but resting potential(RP)remained the same.The increase of APresulted from the increases of overshoot.When the sympathetic nerve wasstimulated although the heart rate increased and the duration of AP wasshortened,the plateau phase of AP was prolonged.These results suggest that theeffects of vagal and sympathetic nerves on the transmembrane potential of cardiacventricular cells are coordinated and the normal characteritics of transmembranepotential are maintained by both the vagal and sympathetic nerves.     

大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

假单胞菌胆固醇酯酶的生产Ⅰ.菌种筛选和发酵条件的研究

通过平板初筛和摇瓶复筛,从371株菌中筛得一株有工业生产价值的胆固醇酯酶产生菌,并对它进行了发酵条件的研究。用所获得的胆固醇酯酶配制的三酶试剂具有良好的稳定性,适用于临床检测。     

1020例不孕症的子宫及输卵管形态学分析

用复方泛影葡胺注射液对1,020例不孕症者作子宫输卵管造影。结果:子宫腔形态异常540例。双侧输卵管通畅但形态异常者268例。单侧输卵管通畅但形态异常者110例。结果表明:子宫输卵管形态异常与不孕有密切关系。     

Subcutaneously administered recombinant human β-interferon in the treatment of chronic hepatitis B virus infection

Background: Treatment of chronic replicative hepatitis B virus (HBV) infection is aimed at stopping viral replication and preventing the development of chronic liver disease. β-Interferon treatment has been less well studied than α-interferon. Methods: The efficacy and tolerability of a 6-month course of subcutaneously administered human recombinant β-interferon (rINF-β_ser) was studied and the results of a low-dose regime compared with a high-dose regime. Twenty patients (17 men and three women), aged 24–54 years, with chronic hepatitis B virus infection (all hepatitis B surface antigen-positive with detectable HBV-DNA in their sera for at least 3 months prior to therapy) were randomized into two treatment groups of 10 patients each. The low-dose group received 6×10⁶ U/dose and the high-dose group received 30×10⁶ U/dose, both groups receiving their respective doses three times a week initially for 1 month and continuing for a total of 6 months. Results: The treatment was well tolerated in both groups. None of the patients required dosage reduction or cessation of treatment because of side-effects. HBV-DNA decreased in all patients during treatment, demonstrating the anti-viral efficacy of rINF-β_ser, and was undetectable in 20 and 40% of patients receiving low-dose and high-dose regimes, respectively, at the end of 6 months treatment (P=N.S.). One year after completion of treatment, HBV-DNA was undetectable in 50 and 30% of patients in the low-dose and high-dose groups, respectively (P=N.S.). However, only one patient achieved seroconversion with loss of the hepatitis B surface antigen and appearance of an anti-hepatitis B ‘e’ antigen at the end of 18 months. Conclusion: This study shows that subcutaneously administered rINF-β_ser is well tolerated, but the optimal dose and duration of treatment still needs to be defined by further studies.