Effects of IL-17 on expression of GRO-α and IL-8 in fibroblasts from nasal polyps

Recent studies indicated that interleukin（IL）-17, growth-related oncogene（GRO）-α and IL-8 play an important role in the pathogenesis of nasal polyps. However, the effects of the increased amount of IL-17 and the production of GRO-α and IL-8 in human nasal polyp fibroblasts are not completely understood. This study aimed to determine the effects of the increased IL-17 on the changes of GRO-α and IL-8 expression in human nasal polyp fibroblasts and further investigate the mechanism of neutrophil infiltration in nasal polyps. Nasal polyp fibroblasts were isolated from six cases of human nasal polyps, and the cells were stimulated with five different concentrations of IL-17. Real-time fluorescence quantitative polymerase chain reaction（RT-PCR） was used to detect the mRNA expression of GRO-α and IL-8. The mRNA of GRO-α and IL-8 was expressed in unstimulated controls and remarkably increased by stimulation with IL-17. Moreover, the levels of GRO-α and IL-8 produced by fibroblasts were increased gradually with the increases in IL-17 concentrations. The present study showed that nasal fibroblasts can produce GRO-α and IL-8, and their production is remarkably enhanced by IL-17 stimulation, thereby clarifying the mechanism of the IL-17 mediated neutrophil infiltration in nasal polyps. These findings might provide a rationale for using IL-17 inhibitors as a treatment for nasal inflammatory diseases such as nasal polyps.     

重型再生障碍性贫血免疫抑制治疗疗效预测指标的研究

目的 探索可能用于重型再生障碍性贫血(再障)免疫抑制治疗(IST)疗效预测的指标.方法 收集37例重型再障患者的静脉血标本,20例健康献血员作为正常对照.采用酶联免疫吸附实验检测血浆内白介素-2(IL-2)及干扰素-γ(IFN-γ)水平,采用序列特异引物聚合酶链反应检测HLA-DRB1*15及HLA-DRB1*1501,结合年龄、性别及粒细胞和红细胞膜表面CD55、CD59表达情况等,对各项指标与疗效的关系进行统计分析.结果 治疗前IL-2水平升高者有效率高于水平降低者(66.7% vs 28.6%,P<0.05);治疗前IFN-γ水平升高者有效率高于水平降低者(73.7% vs 25 0%,P<0.05);HLA-DRB1*15阳性者有效率与阴性者差异无统计学意义(62.5% vs 44.4%,P>0.05);HLA-DRB1*1501阳性者与阴性者有效率均为50%;CD55、CD59表达降低者有效率与表达未降低者差异无统计学意义(80.0% vs 40.0%,P>0.05);年龄≤40岁者有效率高于年龄>40岁者(60.0% vs 14.3%,P<0.05);女性患者有效率高于男性患者(62 5% vs 42 9%,P<0 05).结论 重型再障患者治疗前血浆IL-2、IFN-γ水平、性别及年龄可作为IST疗效预测指标,而HLA-DRB1*15、HLA-DRB1*1501及CD55、 CD59在本研究中不具有预测作用.     

思美泰联合易善复治疗妊娠期胆汁淤积综合征临床观察

目的探讨思美泰联合易善复治疗妊娠期胆汁淤积综合征的临床价值。方法回顾性分析21例ICP患者应用思美泰联合易善复治疗的临床资料。结果思美泰联合易善复治疗ICP能降低ICP患者围产儿的死亡率。结论思美泰联合易善复治疗妊娠期胆汁淤积是一种有效的临床方法。     

温病活血化瘀法与糖尿病血管病变相关性研究

活血化瘀法作为温病常用治疗大法之一,在温病血分证中发挥了举足轻重的作用。将此法运用到糖尿病血管病变中,充分发挥其较为广泛的作用机理,对减少糖尿病及其并发症的产生,延长糖尿病患者寿命,提高生活质量有重要的意义。     

NRG1在肝细胞肝癌中杂合性丢失和蛋白表达的研究

目的：分析在肝细胞性肝癌中NRG1基因的两个多态性位点D8S278和D8S499杂合性丢失（loss ofheterozygosity,LOH）情况及其与NRG1蛋白表达水平之间的关系,探讨NRG1在肝细胞肝癌发生发展中的作用。方法：酚氯仿抽提经石蜡包埋的肝癌和癌旁正常组织DNA,进行PCR扩增,扩增出来的产物进行变性聚丙烯酰胺凝胶电泳并分析两位点杂合性丢失情况;用免疫组织化学方法检测肝癌组织和癌旁正常组织中NRG1的表达情况。结果：D8S278和D8S499的LOH率分别为32.43%（24/74）、37.86%（26/69）,总频率为48.65%（36/74）;NRG1在肝癌组织中的阳性表达率为67.57%（50/74）,显著低于在正常组织中的表达水平（97.30%,72/74）（P〈0.05）,这种降低与NRG1基因多态性位点的LOH有关;NRG1表达下调与肿瘤大小、Edmondson分级相关,而与年龄、性别、HBsAg、AFP、肝硬化和肝内外转移无明显相关。结论：NRG1基因可能是肝细胞肝癌的一个候选抑癌基因,在肝癌发生发展中起一定的作用;可为临床诊断、治疗及预后评估提供理论依据。     

The Prevalence of Nonalcoholic Fatty Liver Disease and its Association with Lifestyle/dietary Habits among University Faculty and Staff in Chengdu

ObjectiveTo investigate the prevalence of nonalcoholic fatty liver disease (NAFLD) in different university categories and its association with lifestyle/dietary habits.MethodsA cross-sectional study was carried out on 9 378 faculty members and staff who participated in an annual health checkup at three universities selected by random cluster sampling. Demographic, anthropometric, biochemical indices and abdominal ultrasound measurements were collected. A nested case-control study was conducted with 200 NAFLD cases and 200 controls matched by gender, age (±3 years), and university.ResultsThe overall prevalence of NAFLD was 10.3% (13.7% in males and 6.8% in females). The prevalence was significantly higher in the science and engineering university (22.1%) than in the comprehensive universities with (6.4%) and without (10.9%) medical colleges. Obesity/overweight, hyperlipidemia, diabetes mellitus, and family history of NAFLD were independently associated with higher risk of NAFLD, as were frequent consumption of desserts and salty/spicy foods. Using nutritional supplements was a protective factor against NAFLD. Intake of coarse cereals, potatoes, vegetables, fruits, and milk was significantly lower, and intake of red meat, viscera, candies and pastries, cooking oil, and total energy was significantly higher in participants with NAFLD than in controls.ConclusionScience and engineering university faculty and staff are key targets for NAFLD prevention. NAFLD is closely associated with age, gender, university type, metabolic diseases, and lifestyle/dietary habits.     

显微精索去神经术治疗特发性慢性睾丸痛的围手术期护理

目的 总结特发性慢性睾丸痛患者行显微精索去神经术的围手术期护理经验.方法 回顾分析在本院接受显微精索去神经术的2例特发性慢性睾丸痛患者的临床资料,包括术前、术后的疼痛评分、心理状况评估及手术切口护理,总结相关护理经验.结果 2例患者术后疼痛明显缓解,无切口感染,心理稳定,均于术后1周左右出院.结论 周密的围手术期护理是此类患者显微手术后顺利康复的重要环节.     

Lacrimal sac diverticulum presenting as a lower eyelid mass with a secreting fistula

To the editor： A 12-month-old child was admitted to our department for treatment regarding his inner canthus mass. His parents complained epiphora and mucopurulent discharge of the left eye after formation of a mass located in the left lower eyelid and medial canthus over 3 months （Figure 1A）. The mass increased in size gradually. This child was diagnosed as acute dacryocystitis and underwent probing for two times in another hospital. When he was 6 months old, he also had a similar mass which had resolved spontaneously. No history of sinus or systemic disease, trauma, or facial surgery was found.     

眼眶骨折微型钛板塑形内固定整复术的疗效探讨

目的：探讨微型钛板塑形内固定整复术在眼眶骨折中的应用及疗效。方法选取2009年2月-2014年3月期间该院接收治疗的眼眶骨折患者共103例为研究对象,根据患者采取的内固定方式将103例患者分为观察组和对照组。其中55例患者采取微型钛板塑形内固定治疗为观察组,48例患者采取羟基磷灰石复合人工骨板内固定治疗为对照组。对比两组患者的愈合情况。结果本次微型钛板塑形内固定整复术中,一次性整复微型钛板固定成功27例,粉碎性眼眶骨折患者进行II期整复微型钛板固定38例,所有患者均成功实施整复固定手术,手术后未出现排斥反应或脱落等并发症。随访3个月,经CT扫描检查,55例复合眼眶骨折,骨性眶壁解剖复位良好,整复材料无不良反应,无脱出、移位及感染。眼球内陷、移位恢复50例,占90.1%。52例中全部生活范围复视消失,3例(5.4%)残留周边复视。对照组中整复材料3例出现不良反应,2例脱出、1例移位并发感染,不良反应发生率为6.2%。眼球内陷、移位恢复36例,占65.5%。观察组患者的恢复率为90.1%,不良反应率为0.0%。对照组患者的恢复率1为65.5%,不良反应率2为6.2%。两组患者在恢复率、不良反应发生率上对比差异有统计学意义(χ21=6.384,P<0.05;χ22=5.937,P<0.05)。结论在眼眶骨折的复位固定处理过程中,采用微型钛板塑形内固定整复术可以很好的对眼眶受损软组织进行整复,该方法操作简单,对患者影响较小,是整复眼眶和颜面部骨折理想材料之一,有利于促进患者眼球运动功能的恢复。     

改良"二袖套法"制备大鼠原位肝移植动物模型

目的：介绍改进的“二袖套法”法大鼠原位肝移植动物模型。方法：采用改进的“二袖套”法进行110只大鼠原位肝移植。结果：切取供肝时间平均37min，修肝时间平均13min，无肝期平均14min，术中和术后4h内死亡8只，手术成功率达93%。术后1周死亡11只，1周存活率为89%，主要死亡原因为感染和胆道梗阻。结论：改进的“二袖套”法大鼠原位肝移植具有无肝期短、手术成功率高，大鼠术后存活时间长的优点，是大鼠原位肝移植的理想术式。