基质金属蛋白酶9抑制剂对人舌鳞癌SCC-25细胞侵袭、迁移能力的影响及机制探讨

目的:探讨基质金属蛋白酶9(MMP-9)抑制剂对人舌鳞状细胞癌(TSCC)SCC-25细胞侵袭、迁移能力的影响及其相关机制.方法:取对数生长期SCC-25细胞,分别采用0、5、10、20μmol/L的MMP-9抑制剂BB-94干预,分别设为对照组、实验A组、实验B组和实验C组.采用RT-qPCR和Western免疫印迹...     

神经内科门诊患者焦虑障碍、睡眠障碍与应对方式的关系

目的 调查神经内科门诊患者焦虑障碍与睡眠障碍之间的关系,并探讨应对方式在二者之间的潜在中介作用。方法 以2019年1—12月该院就诊的102例神经内科门诊患者为研究对象。分别采用综合医院用焦虑量表、匹兹堡睡眠指数问卷和应对方式问卷评估患者的焦虑障碍、睡眠障碍与应对方式。结果 焦虑障碍与睡眠障碍呈正相关(r=0.592,P=0.003)。多元线性回归分析显示,成熟应对方式如问题解决(β=—0.493,P=0.002)与混合应对方式如合理化(β=—0.336,P=0.021)对睡眠障碍与焦虑障碍的关系具有负向预测作用,而不成熟应对方式如幻想(β=0.237,P=0.041)、退避(β=0.223,P=0.040)对睡眠障碍与焦虑障碍的关系具有正向预测作用。结论 有效应对方式能够缓解焦虑障碍对睡眠障碍的负面影响。     

急诊腹部手术术后发生脓毒症的危险因素分析

目的 探讨急诊腹部手术患者术后发生脓毒症的危险因素。方法 回顾性分析2010—2019年该院接受急诊手术的9 104例患者资料,根据术后是否发生脓毒症分为脓毒症组(324例)和非脓毒症组(8 780例)。收集两组术前临床特征、术前实验室检查、术后并发症和死亡率,并进行多因素分析以评估危险因素。结果 术后脓毒症发生率为3.6%,脓毒症组死亡率明显高于非脓毒症组(17.9%vs. 1.2%),差异有统计学意义(P<0.05)。年龄>60岁(OR=1.02,95%CI:1.01～1.02)、男性(OR=1.22,95%CI:1.15～1.42)、ASA分级>3级(OR=1.98,95%CI:1.46～2.35)、合并病态肥胖(OR=1.03,95%CI:1.01～1.27)、COPD(OR=2.11,95%CI:1.95～2.85)和糖尿病(OR=1.28,95%CI:1.17～1.44)、术后急性肾衰或透析(OR=4.56,95%CI:2.53～7.42)、术区感染(OR=4.21,95%CI:2.77～9.05)是术后发生脓毒症的危险因素(P<0.05)。胆道、胰...     

Effects of Chronic Exposure to Diets Containing Moldy Corn or Moldy Wheat Bran on Growth Performance,Ovarian Follicular Pool,and Oxidative Status of Gilts

Background: We investigated the effect of replacing normal corn (NC) or normal wheat bran (NW) with moldy corn (MC) or moldy wheat bran (MW) on growth, ovarian follicular reserves, and oxidative status. Methods: Sixty-three Landrace × Yorkshire gilts were assigned to seven diets formulated by using MC to replace 0% (control), 25% (25% MC), 50% (50% MC), 75% (75% MC), and 100% NC (100% MC), MW to replace 100% NW (100% MW), and MC and MW to replace 100% NC and 100% NW (100% MC + MW), from postnatal day 110 to day 19 of the second estrous cycle. Results: Feeding the gilts with MC or MW induced a lower average daily gain at days 29–56 of the experiment. Age at puberty remained unchanged, but MC inclusion resulted in a linear decrease in antral follicles with diameter >3.0 mm, and control gilts had a 12.7 more large antral follicles than gilts in the 100% MC + MW treatment. MC inclusion linearly decreased the numbers of primordial follicles, growing follicles, and corpora lutea, associated with a lower anti-Müllerian hormone level in serum and 17β-estradiol level in follicular fluid. MC inclusion decreased the serum concentrations of insulin-like growth factor 1 and its mRNA levels in the liver, combined with higher malondialdehyde concentration and lower total superoxide dismutase activities in serum and liver. Conclusion: Chronic exposure to MC-containing diets caused the loss of follicles, even if levels of deoxynivalenol, zearalenone, and aflatoxin B1 were below the levels allowed by China and Europe standards.     

Microstructure of Dolostones of Different Geological Ages and Dedolomitization Reaction

Dolostone is widely distributed and commonly used as concrete aggregates. A large number of studies have shown that there are significant differences in the expansibility of different dolostones, and the key factors determining the expansibility of alkali carbonate rocks have not been clarified. In this paper, rocks were selected from five different geological ages: Jixianian, Cambrian, Ordovician, Devonian, and Triassic ages. The ordering degree and the content of MgCO₃ of dolomites in rocks of different geological ages were determined by X-ray diffraction (XRD). The degree of dedolomitization reaction in rocks cured in 80 °C, 1 mol/L NaOH solution was determined by quantitative X-ray diffraction (QXRD). The morphology of dolomites in rocks was determined by a polarizing microscope. The products of the dedolomitization reaction were determined by field emission electron microscopy (FESEM-EDS). According to the test results, the following conclusions are drawn. There is a good positive correlation between ordering degree and the molar fraction of MgCO₃ of dolomites. When the MgCO₃ mole fraction of dolomites varies from 47.17% to 49.60%, the higher the MgCO₃ mole fraction, the greater the ordering degree of dolomite. By analyzing the degree of the dedolomitization reaction of different dolostone powders cured at 80 °C in 1 mol/L NaOH solution, it is found that the older the geological age of dolostone, the slower the dedolomitization reaction rate and the lower the degree of dedolomitization reaction. The lower the ordering degree of dolomite crystal in the same geological age, the faster the rate of dedolomitization reaction and the higher the degree of dedolomitization reaction.     

The Effect of Pore Structure on Impact Behavior of Concrete Hollow Brick,Autoclaved Aerated Concrete and Foamed Concrete

Porous concrete is an energy absorption material, which has been widely used in civil engineering, traffic engineering and disaster reduction engineering. However, the effect of pore structure on the impact behavior of the porous concrete is lacked. In this study, a series of drop-weight impact tests were carried out on three typical types of porous concrete, i.e., concrete hollow brick (CHB), autoclaved aerated concrete (AAC) and foamed concrete (FC), to investigate the effect of pore structures on their impact behavior. For comparison, static load tests were also conducted as references. According to the damage to the samples, the developments of impact force, strain, contact stress–strain relationship and absorbed energy during drop-weight during the impact test were measured and analyzed. The results show that the ratio between the peak impact stress and compressive strength of CHB was 0.44, while that of AAC and FC increased to about 0.6, indicating that the small and uniform pore structure in AAC and FC had a higher resistance against impact load than the hollow cavity of CHB. In addition, the elastic recovery strain in AAC increased by about 0.2% and its strain at peak contact stress increased by about 160% for a comparison of CHB, implying that a small open pore structure could enhance ductility. Besides, the peak contact stress of FC was close to that of AAC during impact loading, while the strain at peak contact stress of FC increased by about 36% compared with AAC, revealing that the closed-pore structure could further enhance the deformation potential. Correspondingly, the energy absorption rates of CHB, AAC and FC were 85.9 kJ/s, 54.4 kJ/s and 49.7 kJ/s, respectively, where AAC decreased by about 58% compared with CHB, and FC decreased by about 10% compared with AAC.     

微血管计数在子宫内膜癌中的意义

目的:探讨不同病理类型子宫内膜癌中微血管密度与临床病理的关系.方法:采用S-P免疫组化染色法检测29例雌激素依赖型(Ⅰ型)子宫内膜癌及23例非雌激素依赖型(Ⅱ型)子宫内膜癌中CD34的表达以及计数微血管密度(Micro Vessel Density,MVD).结果:Ⅰ型子宫内膜癌的MVD 76.9±31.2,Ⅱ型子宫内膜癌MVD 131.65±35.63,有显著统计学差异(P＜0.001).低分化子宫内膜癌组MVD显著高于高、中分化子宫内膜癌组,差异具有统计学意义(P＜0.05).Ⅰ型子宫内膜癌中MVD和FIGO分期、子宫外转移和淋巴结转移有相关性.Ⅱ型子宫内膜癌中MVD与上述子宫内膜癌临床病理因素之间均无相关关系.结论:MVD与子宫内膜癌的发生有关,可作为Ⅰ型子宫内膜癌预后判断的参考指标.Ⅱ型子宫内膜癌可能在发病、发展、侵袭和转移过程中,具有不同于Ⅰ型子宫内膜癌的生物学模式.     

CT联合血清肿瘤标志物对早期肺癌的诊断价值

目的 探讨CT联合血清肿瘤标志物对早期肺癌的诊断价值。方法 选取102例早期肺癌患者和102例肺部良性疾病患者,分别作为恶性组和良性组,比较两组患者的血清肿瘤标志物[胃泌素释放肽前体（ProGRP）、癌胚抗原（CEA）、糖类抗原211(CA211)、神经元特异性烯醇化酶（NSE）]水平。以手术病理或纤维支气管镜活检为金标准,分析CT和血清肿瘤标志物单独及联合应用对早期肺癌的诊断效能及其与金标准的一致性,采用受试者工作特征（ROC）曲线分析CT和血清肿瘤标志物单独及联合应用对早期肺癌的诊断价值。结果恶性组患者的血清ProGRP、CEA、CA211、NSE水平均明显高于良性组,差异均有统计学意义（P﹤0.01）。CT和血清肿瘤标志物联合应用对早期肺癌的诊断结果与金标准具有高度一致性（P﹤0.05）,灵敏度、特异度和准确度均高于二者单独应用的诊断结果。CT和血清肿瘤标志物联合应用对早期肺癌具有较高诊断价值（AUC=0.980）,单纯CT对早期肺癌具有中等诊断价值（AUC=0.863）,单纯血清肿瘤标志物对早期肺癌具有较高诊断价值（AUC=0.907）,CT和血清肿瘤标志物联合应用的诊断价值最...     

Evaluation of blood pressure lowering effect by generic and brand-name antihypertensive drugs treatment: a multicenter prospective study in China

Background::Generic drugs are bioequivalent to their brand-name counterparts; however, concerns still exist regarding the effectiveness and safety of generic dr...