Anti-inflammatory effect of methoxyphenamine compound in rat model of chronic obstructive pulmonary disease

AIM: To evaluate the anti-inflammatory effect of methoxyphenamine compound (MC) on chronic obstructive pulmonary disease (COPD) in rats by measurement of proinflammatory cytokines, total and differential white cell counts (WCC) of bronchroalveolar lavage fluid (BALF). METHODS: Adult rat model of COPD (COPD group) was induced by intratracheal instillation of lipopolysaccharides and exposure to cigarette smoke. Treatment groups received different dosage of MC (3, 9, and 27 mg daily, MC group) or prednisone (0.25 mg daily, P group) respectively. Tumor necrosis factor alpha (TNF-α), interleukin 1beta (IL-113), interleukin-6 (IL-6), transforming growth factor β (TGF-β) of BALF were determined by ELISA. Total and differential WCC were performed after Giemsa staining. RESULTS: The levels of TNF-α, IL-1β, IL-6, TGF-β, total and differential WCC in BALF of MC groups were significantly decreased than that of COPD group (P<0.01), and there was no significant difference among MC groups. There was no significa     

脾切除对大鼠血浆及肝肺组织中内毒素廓清的影响

目的 观察脾切除对血浆中内毒素清除的影响及内毒素在主要脏器的分布特征,探索脾切除后上反应与组织损害的发生机制。方法 雄性Ｗｉｓｔａｒ大鼠１１２只,随机分为有脾组（ｎ＝５６,行大网膜切除术,保留脾脏）、脾切除组（ｎ＝５６,行脾切除术）。术后１周静脉注射内毒素０．１ｍｇ／ｋｇ,分别于注射前、注射后１０ｍｉｎ、０．５、１．５、４、１２、２４ｈ活杀动物,测定血浆和肝、肺组织内毒素水平,同时检测肝、肺脏器功     

VEGF、KDR的表达对肝癌的血管生成、生长、转移的影响

目的　研究血管内皮生长因子 (VEGF)及其受体KDR(kinaseinsertdomaincontainingre ceptor)对肝细胞肝癌的血管生成、生长、转移等方面的影响。　方法　对 45例肝癌、2 1例肝硬化、8例正常肝标本进行了免疫组化、原位杂交染色 ,然后行图像分析 ,观察VEGF、KDR的表达与肝癌病理特点之间的关系 ,并进行统计学分析。　结果　肝细胞肝癌中VEGF高表达 ;并与肝癌的血管生成相关 ;VEGF的表达强度与表达率均与肝癌的转移、包膜形成相关 ;KDR不仅表达于血管内皮细胞 ,还表达于部分肝癌细胞。　结论　VEGF在肝癌的血管生成、癌细胞转移、包膜形成中可能起到重要作用 ,KDR在肝癌细胞的表达可能是一种自分泌行为。     

TGF-beta-1 gene transfer in joint cartilage cells. Stimulating effect in extracellular matrix synthesis

TGF beta-1 has been shown to upregulate matrix synthesis in articular chondrocytes. TGF beta-gene transfer to chondrocytes has the potential to increase the local production of this key component within regenerating cartilage after trauma and could support the repair process in articular cartilage lesions. Primary rabbit articular chondrocytes were cultured and retrovirally transfected with the experimental TGF beta-1 and the lacZ marker gene for control purposes. After radioactive labeling of new synthesized matrix proteins results were compared with normal primary chondrocytes. After TGF beta-1 gene transfer the endogenous growth factor concentration was doubled compared to normal chondrocytes and decreased in the lacZ control group. The proteoglycan synthesis in TGF beta-1 transfected chondrocytes showed a 96% increase compared to the basal production of normal chondrocytes. The LacZ transfected group revealed the opposite effect by a 44% decrease. The collagen synthesis of TGF beta-1 transfected chondrocytes was 304% compared to normal chondrocytes, predominantly type II collagen. The lacZ group collagen production was reduced by 35%. We conclude that TGF beta-1 gene transfer overcomes the decreasing effect observed by transfection with the LacZ marker gene and increases matrix synthesis in articular chondrocytes. Genetically altered chondrocytes might improve the repair of cartilage lesions by stimulating matrix synthesis and supporting the expression of the hyaline phenotype.     

Tissue engineering and gene therapy of the musculoskeletal system with muscle cells

AIM: Muscle-based somatic gene therapy is a novel way to alleviate a biochemical deficiency. METHOD: Muscle-derived cells are very promising in the field of gene therapy and tissue engineering. First, most muscle tissue is accessible by injection. Second, muscle tissue consists of multinucleated, postmitotic myofibers, which enable a long-term expression of the transduced gene. Third, muscle tissue can be biopsied easily. It is available in abundance and the biopsy does not compromise the health and function of the patient. Finally, muscle tissue is highly vascularized, which makes systemic delivery feasible. RESULTS: Muscle-derived cells can promote muscle healing and bone healing. Implanted cells maintain a long-term transgene expression of therapeutic proteins. Isolated, muscle-derived stem cells can differentiate in osteoblasts. CONCLUSION: Based on these characteristics, we present four possible applications: inherited muscular diseases, muscle injury, bone healing, and intraarticular disorders.     

卫星搭载药用植物曼陀罗遗传变异的随机扩增多态性DNA分析

以曼陀罗种子为材料探讨太空环境对药用植物的影响。方法利用返回式卫星搭曼陀罗的种子,返回地面后播于实验田中,应用随机扩增多态性ＤＮＡ（ＲＮＡ（ＲＡＰＤ）分子标记技术分析不同组曼陀罗基因组的变异情况。结果从６５个供试引物中筛选出１５个能够产生可重复多态性增产物的引物。与地面对照组相比,失重组共产生３９条多态性片段,基因组的多态性频率为２３．１％,击中组共产生４５条多态性片段,基因组的多态性频率为２４．     

三氧化二砷诱导人肿瘤细胞凋亡和G2＋M期阻滞但引起人永 …

目的　研究 As2 O3引起肿瘤细胞凋亡和对细胞周期的影响。方法　采用 DNA凝胶电泳 ,流式细胞仪分析、RT- PCR和 Western免疫印迹等技术检测细胞凋亡的发生。结果　由 As2 O3诱导的人肺腺癌 GL C- 82、胃腺癌 MGC- 80 3和 SGC- 790 1、食管癌 Ec10 9、宫颈癌 He L a细胞凋亡前 ,细胞阻滞在 G2 M期 ,上述细胞均表现为对c- myc基因的下行调节 ;但在导致永生化人宫颈上皮 HCE16 /3细胞凋亡之前 ,细胞却被阻滞在 G1期 ,对 c- m yc基因表达无影响。结论　 As2 O3引起细胞凋亡与细胞周期阻滞有关 ,在肿瘤细胞和前恶变的 HCE16 /3细胞中阻滞的时期不同 ,这可能与 c- myc基因表达的改变与否有关     

Direct angioplasty of totally occluded left main coronary artery in acute myocardial infarction complicated by cardiogenic shock: report of two cases and literature review

Acute total occlusion of the left main coronary artery is usually characterized by a rapid course of deterioration that challenges therapeutic intervention. Unlike subtotal occlusion of the left main coronary artery, acute total occlusion of the left main coronary artery is extremely rare and has a grave prognosis. Most patients with this problem die suddenly or go into cardiogenic shock. Direct percutaneous transluminal coronary angioplasty (PTCA) was performed on 2 patients, both suffering from cardiogenic shock due to acute total occlusion of the left main coronary artery. Both patients underwent coronary artery bypass surgery subsequently. One patient, who had substantial intercoronary collaterals, remained asymptomatic at 31 months of follow-up. The other, who had no intercoronary collateral circulation, expired 3 days after coronary artery bypass surgery. We conclude that direct PTCA to the acutely occluded unprotected left main coronary artery in cardiogenic shock patients is a potentially life-saving procedure, and the presence or absence of collaterals from the dominant right coronary artery will influence the clinical outcome.