情感性精神障碍事件相关电位变异与自杀行为史的相关性

探讨情感性精神障碍患者件相关电位变异的临床意义。方法收集３９例抑郁相和２２例躁狂相患者,以３３例正常人为对照组,记录其事件相关电位Ｐ３００和ＣＮＶ。结论Ｐ３００和ＣＮＶ临床应用价值得到初步证实,自杀行为相关的生物学指标研究中需进一步重视。     

双功能超声和磁共振血管造影及数字减影血管造影评估颅外颈？…

评价双功能多普勒超声,磁共振血管造影和数字减影血管造影诊断颅外颈内动脉狭窄的相关性。方法３２例患者同时接受颈动脉多普勒超声、ＭＲＡ和ＤＳＡ检查。狭窄度计算方法同ＮＡＳＣＥＴ。结果超声和ＭＲＡ检查中未出现并发症,１例（３．１％）在ＤＳＡ术后出现脑梗塞症状。彩色多普勒与ＤＳＡ比较,Ｋａｐｐａ＝０．７８,超声诊断重度狭窄和中度以上狭窄的敏感度,特异度,准确度分别是７８％、９６％、９２％和９７％、９１％、     

The prevalence of NIDDM and IGT and related factors among residents in some areas of Hubei Province, China

The epidemiological survey of prevalence of NIDDM (non-insulin dependent diabetes mellitus) and IGT(impaired glucose tolerance) was conducted among 9450 residents aged 25-70 in some areas of Hubei Province, China. The results show that NIDDM and IGT prevalences are 2.62% and 4.48%, respectively. There is no significant difference between male and female (P > 0.05). The NIDDM prevalence in cities is slightly higher than that in countryside, but the difference is not significant (P > 0.05). However, the IGT prevalence in city is significantly higher than that in countryside (P < 0.01). The prevalence of both NIDDM and IGT is increasing along with the age of the population. It is also significantly related to the family history of NIDDM, hypertension, and high body mass index (BMI). By using stepwise logistic regression to analyse the risk factors of NIDDM, age (OR = 1.86), BMI(OR = 2.69), family history (OR = 2.84) and hypertension (OR = 2.23) entered the model (significance level is alpha = 0.05).     

Adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose X-ray irradiation in mice

The dose-effect of adaptive response of thymocyte apoptosis and cell cycle progression induced by whole-body X-ray irradiation (WBI) was studied in male Kunming mice. The inductive doses (D1) were 25, 50, 75, 100 or 200 mGy 6 h before the challenging doses (D2) of 1.0, 1.5 or 2.0 Gy. The changes in the percentages of the thymocyte apoptotic bodies (TAB) and the cells in different phases of cell cycle were measured with flow cytometry. The percentages of TAB decreased, the arrests of G1 and G2 + M phases diminished, and the cells of DNA synthesis of S phase increased when the D1 + D2 groups was compared with the D2 groups. When D1 was 200 mGy, the adaptive response of thymocyte apoptosis and cell cycle progression were no longer induced by low dose radiation (LDR). In addition, the extracellular fluid from the splenocytes were cultured with Con A for 48 h in vitro 24 h after 75 mGy WBI was placed in the murine thymocyte suspension from mice irradiated with 2.0 Gy WBI and co-incubated. The thymocyte apoptosis decreased. Especially, noteworthy was that the percentages of TAB after the incubation for 72 h were significantly lower than those in 2.0 Gy irradiated thymocytes (P < 0.05). These results indicate that when the mice were irradiated with 25-100 mGy (D1, 12.5 mGy/min) 6 h before 1.0-2.0 Gy (D2, 0.287 Gy/min) exposure, an adaptive response of thymocyte apoptosis and cell cycle progression may be induced under the condition of WBI, and LDR (75 mGy) may change the microenvironment of immune cells and decrease the thymocyte apoptosis.     

重组人肝细胞生长因子基因的克隆

用ＲＴ－ＰＣＲ技术从人胎盘组织内成功地扩增全长肝细胞生长因子（ＨＧＦ）ｃＤＮＡ基因（２ １８４ｂｐ）,并将其克隆至ｐＧＥＭ－Ｔ载体,经限制性核酸内切酶ＮｄｅⅠ,ＢｇｌⅡ,ＨｉｎｄⅢ,ＢａｍＨⅠ和ＸｈｏⅠ的酶谱分析和ＤＮＡ测序分析证实。再将其亚克隆至逆病毒载体ｐＬＮＬ－ＸＨＣ,可进一步用于基因表达和基因治疗的研究。     

肾细胞癌中FHIT基因的研究

目的：探讨肾细胞癌中ＦＨＩＴ基因的改变及意义。方法：用ＲＴ－ＰＣＲ及Ｓｏｕｔｈｅｒｎ印迹杂交法检测肾细胞癌及其细胞系中ＦＨＩＴ基因转录本,并观察其基因组的改变。结果：约８．３％（２／２４）肾细胞癌有异常转录本;其中一株细胞系ｃＤＮＡ在用外引物扩增时,只有一个变小的ＦＨＩＴ基因异常转录本;用内引物扩增时,ＦＨＩＴ基因转录本完全缺失。约２０％（５／２４）肾细胞癌ＦＨＩＴ基因区域的基因组ＤＮＡ出现重排。     

中药“拮新康”对白血病细胞凋亡的影响

为探讨中药“拮新康”对白血病细胞凋亡的影响。采用形态学及ＤＮＡ凝胶电泳方法观察其对白血病细胞株ＨＬ－６０及Ｋ５６２细胞凋亡的影响,结果示：①电镜观察可见凋亡细胞和凋亡小体,ＤＮＡ凝胶电泳可见典型的梯形带改变;②一定浓度范围内的拮新康经适当作用时间后可诱导白血病ＨＬ－６０,Ｋ５６２细胞凋亡,但诱导Ｋ５６２细胞凋亡所需时间较ＨＬ－６０长且剂量较大。提示拮新康可诱导白血病细胞凋亡,与剂量和时间有一定的关     

缺血预处理降低心脏瓣膜置换病人肌钙蛋白T的释放

采用近年来对急性心肌损伤诊断有高度特异性和敏感性的血清标记物——心脏肌钙蛋白T(cTnT)来判断缺血顸处理对心肌缺血再灌注损伤的影响。选择风湿性心脏病瓣膜置换术患者32例,随机分为顸处理组(实验组)和对照组。两组在疾病种类、术中升主动脉阻断及转流时间等方面无明显差异,分别测定术前,术后4h,2,4h及72h血清cTnT含量。结果显示。缺血顸处理组不但降低术后血清cTnT升高的峰值,而且使cTnT的含量较快地恢复至正常水平。提示缺血预处理对心脏缺血再灌注损伤有一定的保护作用。     

全科医生社区卫生服务中的工作范畴与方式的探讨

社区卫生服务对象以老人、妇女、儿童为主 ,疾病所涉及的面广 ,以慢性疾病为主。我们选用全科医生从事此项工作 ,依靠三级甲等省级医院的技术优势 ,立足于本社区 ,开展预防保健 ,巡诊随访 ,开办家庭病房等项目 ,从心理护理到疾病治疗的整体服务方式。使儿童计划免疫率达 10 0 %。做好科普宣传 ,杜绝管区内甲类传染病的发生 ,为管区居民建立健康档案 ,沟通与居民的联系 ,做到小病在社区 ,大病到医院。在心脑血管急症发作时 ,可就地抢救后护送到省医院进一步治疗 ,由于抢救及时 ,使死亡率有所下降。对慢性疾病 ,开设家庭病房 ,在达到与住院相同治疗效果 ,减少病人的经济负担 ,同时也降低了病人再次住院率     

血管内皮生长因子基因在正常造血细胞中的表达

目的　探讨正常造血细胞中血管内皮生长因子 (VEGF)基因的表达水平。方法　TRIzol一步法提取正常外周血和非恶性骨髓标本单个核细胞及正常血小板的总RNA ;以 β2 -微球蛋白基因为内对照 ,逆转录聚合酶链反应 (RT -PCR)半定量分析VEGF基因的表达。结果　 2 2份外周血标本有 18份表达VEGF基因 ,表达率为81.8% ,其中 8份为中度表达 ;18份骨髓标本VEGF基因的表达率高达 94.4% (17/ 18) ,其中高度表达和中度表达分别为 5份与 6份 ;血小板有VEGF基因的低度表达。结论　正常造血细胞可表达VEGF基因 ,提示体内血管生成受到多种效应细胞的共同调节