重组人HMGN2在大肠杆菌中表达及其体外抗乙型肝炎病毒活性研究

目的 制备重组人高迁移率非组蛋白N2蛋白(HMGN2),研究重组HMGN2的抗乙型肝炎病毒(HBV)活性.方法 通过RT-PCR分离纯化人HMGN2基因,并将其构建到原核表达质粒pGEX-4T-1中,经IPTG诱导表达.产物经GST蛋白纯化系统进行纯化,用SDS-PAGE及Western blot分析鉴定.采用MTT法...     

心房肌间隙连接重构与风湿性瓣膜病患者心房颤动的关系

目的　探讨风湿性瓣膜病慢性心房颤动 (房颤 )患者心房肌组织中连接蛋白 4 0 (Cx4 0 )和连接蛋白 4 3(Cx4 3)蛋白质表达和分布的变化与慢性房颤的关系。方法　 32例风湿性心脏病患者 ,其中慢性房颤患者 2 1例 ,窦性心律者 11例 ,瓣膜置换术时取右心耳组织。用免疫荧光双标共聚焦显微镜和Western印迹观察Cx4 0和Cx4 3蛋白质表达和分布的改变。结果　激光共聚焦显微镜计算的Cx4 0荧光斑面积反映了蛋白表达水平 ,慢性房颤组为 0 6 7μm2 / μm3 ± 0 0 9μm2 / μm3 ,显著低于窦性心律组的 1 4 5 μm2 / μm3 ± 0 16 μm2 / μm3 (P <0 0 1) ,且细胞端端连接减少较侧侧连接处严重 ,分布呈现不均一性。Cx4 3总体表达水平未变 ,慢性房颤组为 1 38μm2 / μm3 ± 0 14 μm2 / μm3 ,窦性心律组为 1 5 3μm2 / μm3 ± 0 2 7μm2 / μm3 ,但侧侧分布增加而横向分布减少。Western印迹反映的Cx4 0和Cx4 3蛋白质表达水平与激光共聚焦观察结果一致。结论　心房肌Cx4 0和Cx4 3蛋白质水平降低和再分布可能与风湿性瓣膜病慢性房颤的发生和维持有关。     

InfluenceofQingdaiCompoundCapsule（复方青黛胶囊）ontheExpressionofc－mycinPsoriaticKeratinocytes

ＩｎｆｌｕｅｎｃｅｏｆＱｉｎｇｄａｉＣｏｍｐｏｕｎｄＣａｐｓｕｌｅ（复方青黛胶囊）ｏｎｔｈｅＥｘｐｒｅｓｓｉｏｎｏｆｃ－ｍｙｃｉｎＰｓｏｒｉａｔｉｃＫｅｒａｔｉｎｏｃｙｔｅｓＩｎｆｌｕｅｎｃｅｏｆＱｉｎｇｄａｉＣｏｍｐｏｕｎｄＣａｐｓｕｌｅ（复方青黛...     

~（99m）Tc标单抗在荷人卵巢癌裸鼠体内T／N比值的观察

目的：找出在注射标记抗体的荷瘤裸鼠体内的￣（９９ｍ）Ｔｃ注射剂量、时间与Ｔ／Ｎ比值的关系。方法：用γ计算机和γ计数器测定肿瘤和其它组织的放射性强度，计算Ｔ／Ｎ比值和定位指数ＬＩ．结果：（１）２ｍｃｉ的￣（９９ｍ）Ｔｃ剂量Ｔ／Ｎ比值高；（２）注射标记抗体２８ｈ后，Ｔ／Ｎ比值高，是放免显象的适宜６时间。结论：￣（９９ｍ）Ｔｃ剂量大小与Ｔ／Ｎ比值不呈线性关系，但Ｔ／Ｎ比值与定位指数呈正相关。     

胃食管反流病：症状、内镜表现及食管pH监测关系分析

目的：比较及分析不同症状与黏膜损伤、病理性酸反流的关系。方法：选取2007年3月至2010年12月间于我院就诊、同时接受RDQ问卷调查、胃镜及动态食管pH监测的患者300例,分析症状、食管炎与食管pH变化三者的关系。结果：1. 男性、体重指数、食管裂孔疝是反流性食管炎的危险因素;年龄与重度反流性食管炎相关;2.问卷、内镜及食管pH监测结果缺乏一致性,但反流性食管炎患者重度黏膜损伤与病理性酸反流相关;3.胸痛症状与反流性食管炎及病理性酸反流未见明显相关性。结论：男性、高龄、体重指数、食管裂孔疝可能是食管炎的危险因素;内镜及食管pH监测各有其特点,在诊断中互相补充;与其他症状相比,胸痛患者食管炎或病理性酸反流的发生率下降。     

局部亚低温治疗急性脑出血的临床研究

目的　探讨局部亚低温治疗脑出血的临床疗效。方法　将 40例脑出血患者按 1∶1配对随机分为低温组 2 0例和常规组 2 0例。常规组给予脱水药抗高血压药等 ,低温组在常规组治疗的基础上以医用可控式半导体脑保护制冷仪给予局部亚低温治疗 48h。观察两组间脑水肿量和神经功能缺损的差别。结果　入院时两组之间脑水肿量、欧洲卒中评分比较差异均无显著意义 (P >0 0 5 ) ;治疗 1周、2周时脑水肿量低温组为 17 4ml± 6 2ml和 13 1ml± 5 8ml,对照组为 33 8ml± 16 0ml和2 2 4ml± 12 2ml,两组之间脑水肿量比较差异均有显著意义 (P <0 0 5 ) ;治疗 1周、2周时欧洲卒中评分低温组为 6 2 10± 10 84分和 70 3± 10 7分 ,对照组为 5 2 8± 10 9分和 6 0 5± 10 9分 ,两组欧洲卒中评分之间差异均有显著意义 (P <0 0 5 ) ;应用局部亚低温治疗的患者均无不良反应。结论　局部亚低温结合常规治疗对脑出血患者具有明显脑保护作用 ,使用上安全、可靠。     

肝尾状叶原发性肝细胞肝癌的外科治疗

目的 探讨尾状叶原发性肝癌手术切除的方式及其影响。方法 自1995年至2003年,对39例尾状叶原发性肝癌进行了手术切除,其中单独尾状叶切除19例,联合切除20例。并对两组病例中可能影响术后肝功能的指标进行了比较。结果 39例患者均被成功切除肿瘤,1例于术后30d因肾功能衰竭死亡,3例并发胸腔积液,4例并发腹水,1例并发胆漏,其余病例均顺利恢复。术后30例获得随访,1年、3年、5年生存率分别为53％、50％、39％。结论 尾状叶切除是治疗原发于尾状叶肝癌的有效手段,若肿瘤原发于肝尾状叶而又无其他肝叶侵犯时,单独尾状叶切除该是外科治疗的最佳选择。     

A PCR-basedRapidNeutralizationAssay for GII.4 NorovirusInfection in HIEC6CellCulture

Because of limited viral replication and lack of cytopathic effect in cell culture,a new PCR-based rapid seroneutralization assay for detection of GII.4norovirus neutralized antibodies was developed with serum samples from acute-phase patients,convalescent-phase patients and healthy controls.According to this study,neutralizing antibodies were detected in 100% of     

血红蛋白基纳米氧载体:结构特点、高铁含量控制及血循环中持久存在(英文)

Due to the continuously insufficient supply and the disadvantages associated with the donated blood,artificial blood substitute has received extensive attentions throughoutthe world in recentyears.Nano-structured hemoglobin-based oxygen carriers (NHOCs),in particular the liposome-encapsulated hemoglobin (LEH) and hemo-globin-based polymeric nanoparticles (HbPNPs),which combine the nanotechnology with the traditional cellular oxygen carriers,have shown great potential applications in blood transfusion,thrombus and cancer therapy as well.The present article firstly reports on the current status of the synthesis,properties,clinical trial and application of the LEHand HbPNPs.And then,attentions are devoted to highlight the main challenges and the proposed strategies of the NHOCs with the respect of suppression of the methemoglobin formation and prolonging the blood circulation time.These results make us confident in advancing further development of NHOCs,with the expectation of their eventual commercialization.     

Association between CISH polymorphisms and spontaneous clearance of hepatitis B virus in hepatitis B extracellular antigen-positive patients during immune active phase

Background Some hepatitis B extracellular antigen （HBeAg）-positive chronic hepatitis B （CHB） patients in their immune active phase can clear the virus spontaneously and enter into an inactive hepatitis B virus （HBV） carrier state,indicating a benign prognosis.In this study,the association between cytokine-inducibie SRC homology 2 domain protein （C/SH） gene polymorphisms at-292 （rs414171） and the spontaneous clearance of HBV in HBeAg-positive CHB patients in immune the active phase was investigated.Methods Seventy HBeAg-positive CHB patients in the immune active phase were followed up for 76 weeks without antiviral therapy.The alanine transaminase,aspartate transaminase,HBV DNA,HBeAg and hepatitis B extracellular antibody levels were tested regularly.At week 76,27 patients were classified into group A （HBV DNA level below 2 104 IU/ml and the value of HBeAg declined below 10％ of the baseline at week 76）,and 43 patients were classified into group B （HBV DNA level higher than 2×104 IU/ml or the value of HBeAg did not decline substantially at week 76）.CISH （rs414171） polymorphisms were also tested using the iPLEX system.Results The HBV DNA levels at week 12 were significantly greater in group B compared with group A （group A：（6.87±1.40） log10IU/ml; group B：（7.61±1.38） log10IU/ml,P=0.034） and the HBeAg values were greater in group B at week 28 compared with group A （P=0.001）.The differences in HBV DNA and HBeAg values increased between the groups over time.Sixteen patients in group A and 11 in group B were genotype AA.Those with genotype AT or TT included 11 in group A and 31 in group B （AA vs.AT and TT,odds ratio 4.10 （95％ confidence interval：1.462-11.491）,P=0.006）.Conclusion CISH gene polymorphisms at-292 （rs414171） are associated with HBV clearance in HBeAg-positive CHB patients in the immune active phase,and AA is a favorable genotype for this effect.